Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,551,783 A→G 7.4% S231P (TCA→CCA)  ebpS ← elastin‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,551,7830AG 7.4% 167.3 / 4.8 72S231P (TCA→CCA) ebpSelastin‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (28/37);  new base G (3/2);  total (31/41)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

CCAAACCTGCTGTTCCAGCACCGATCGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATC  >  CP000730/1551648‑1551915
                                                                                                                                       |                                                                                                                                    
ccAAACCTGCTGTTCCAGCACCGATCGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGt                                                                                                                                  <  2:500818/140‑1 (MQ=255)
      ctgctgTTCCAGCACCGATCGCTGCACCTTTTTGGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTa                                                                                                                            <  1:57980/140‑1 (MQ=255)
         ctgTTCCAGCACCGATCGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCt                                                                                                                         >  1:511505/1‑140 (MQ=255)
           gttacaggggcgtgcGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTGGCttt                                                                                                                       >  2:85403/15‑140 (MQ=255)
           gTTCCAGCACCGATCGCTGCACCTTTTTTGCCATTATGAGGACCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCttt                                                                                                                       <  1:377249/140‑1 (MQ=255)
               cAGCACCGATCGCTGCACCTTTTTTTTTTTTTTTTTGATCTTTAGACTTATCTTGAGATGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGTATTACTTTTGTTTTGTGCGTCATTGGAATGTTTCTTAGTTTTATaa                                                                                                                   >  2:96837/1‑140 (MQ=255)
                aGCACCGATCGCTGCACGTTTTTTGCCATTATGATGCGCTTTAGACTTATCTTGAAACGCTTTATCCACAGTCGAGTTATTCGCCTTGCCAGAATTACGTTTGTTATGAGCGTCCTTTAAAAGATGGTCAGCTTTatcag                                                                                                                  >  2:180186/1‑136 (MQ=255)
                   aCCGATCGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAagcag                                                                                                               <  2:675289/140‑1 (MQ=255)
                     cGATCGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGcc                                                                                                             <  1:540454/140‑1 (MQ=255)
                       agcGCTGCACCTTGCGTGGCATCATGATGATCTTTTGACTTATCTTGAGACCCTTTATCCTCAGTCGAATTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCAt                                                                                                           <  2:37623/138‑1 (MQ=255)
                       aTCGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCAt                                                                                                           >  1:660356/1‑140 (MQ=255)
                        tCGCTGCACCTTTTTTGCCATCATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCTAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGTGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCAtt                                                                                                          <  2:542353/140‑1 (MQ=255)
                         cGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTg                                                                                                         >  2:492471/1‑140 (MQ=255)
                         cGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTg                                                                                                         >  1:58652/1‑140 (MQ=255)
                         cGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCCTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGACTGTTTCTTAGCTTTAGAAGAAGCCATTg                                                                                                         >  2:648841/1‑140 (MQ=255)
                          gCTTCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGTTTTATCCTCTGTCTAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGc                                                                                                        <  1:238191/140‑1 (MQ=255)
                              cACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCa                                                                                                    >  1:467419/1‑140 (MQ=255)
                                ccTTTTTTGCCATTATTATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGc                                                                                                  <  2:73359/140‑1 (MQ=255)
                                ccTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGc                                                                                                  <  2:225835/140‑1 (MQ=255)
                                    ttttTCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCa                                                                                              <  2:95517/140‑1 (MQ=255)
                                     tttGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCAc                                                                                             <  2:210055/140‑1 (MQ=255)
                                       tGCCATTATGTTGATCTTTAGACTGATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGCTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCt                                                                                           <  2:520844/140‑1 (MQ=255)
                                        gCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTTAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCCCCAGCTGCACCTg                                                                                          >  1:119694/1‑140 (MQ=255)
                                        gCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTg                                                                                          <  2:223914/140‑1 (MQ=255)
                                        gCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTg                                                                                          >  2:456762/1‑140 (MQ=255)
                                              cagtagattgttAAACTTTTCTTGTGACGCTTTGTCCTCATTCGTGTTATTCGCCTTGCTCGAATTACTTTTGTTTTGAGCGTCATTTGTATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAacac                                                                                    <  2:37987/130‑1 (MQ=255)
                                                   gatCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACctgct                                                                               >  2:128379/1‑140 (MQ=255)
                                                     tCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGt                                                                             <  1:11094/140‑1 (MQ=255)
                                                             cTTATCTTGAGCCGCGTTATCCTCAGTCGAGTTATTCGCCTTGCCCGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTAGACCAGATGCACCTGCAACACCTGCTGTTCCAGCAc                                                                     >  1:224270/1‑140 (MQ=255)
                                                                  cTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGGATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATa                                                                >  2:536305/1‑140 (MQ=255)
                                                                  cTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTATGTTTTGAGCGTCATTTGGATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATa                                                                >  2:536322/1‑140 (MQ=255)
                                                                    tGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTAATTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGAGGCCATTGCACCGGCTGCACCTGCCACACCTGCTGTTCAAGCACCACTacc                                                              >  2:46748/1‑138 (MQ=255)
                                                                     gagaCGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAgct                                                             >  1:364153/1‑140 (MQ=255)
                                                                       gacttttttTCCTCATTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGTAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAgctgc                                                           <  1:668666/135‑1 (MQ=255)
                                                                         cGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAgctgctg                                                         <  2:514447/140‑1 (MQ=255)
                                                                            tttATCCTCAGTCGTGTTATTCGCCTTGCCAGAATTACTTTTTTTTTGATCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCtt                                                      <  2:176285/140‑1 (MQ=255)
                                                                             ttATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCttt                                                     <  1:456732/140‑1 (MQ=255)
                                                                                 ccTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTa                                                 >  1:424776/1‑140 (MQ=255)
                                                                                   tCAGTCGAGTTATTCGCCTTGCCAGAATTACCTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTAcc                                               <  2:391326/140‑1 (MQ=255)
                                                                                      gTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCAtt                                            <  1:697174/140‑1 (MQ=255)
                                                                                      gTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACAAtt                                            >  1:128986/1‑140 (MQ=255)
                                                                                       tCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTa                                           <  2:58652/140‑1 (MQ=255)
                                                                                         gAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTatg                                         <  2:467419/140‑1 (MQ=255)
                                                                                            ttATTCGCCTCGCCAGCACTACTTTTGATTAAAGAGTCGCTTAAATATATCTTAGCTTTAGAACCAGCGAGTGCACCGGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCCGCTGCTTTTTTACCATTatgatg                                      <  2:604837/140‑1 (MQ=255)
                                                                                                  gCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCTATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCttt                                <  2:671885/140‑1 (MQ=255)
                                                                                                   ccTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCCGCTGCTTTTTTACCATTATGATGTTCTTTa                               >  2:372015/1‑140 (MQ=255)
                                                                                                     ttGCCAGAATTACTTTTGTTTTGAGCGTGATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCGCCAATAACTGCTGCTTTTTTACCATTATGCTGTTGTTTatg                             >  2:132970/1‑138 (MQ=255)
                                                                                                         cAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCa                         >  1:523619/1‑140 (MQ=255)
                                                                                                          aGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCat                        >  1:723825/1‑140 (MQ=255)
                                                                                                            aaTTACTTTTGTTTTGAGCGTCATTTGTATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCatct                      <  1:96837/140‑1 (MQ=255)
                                                                                                             aTTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCatctt                     >  1:299123/1‑140 (MQ=255)
                                                                                                              ttACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCatcttg                    <  1:171703/140‑1 (MQ=255)
                                                                                                              ttACTGTTGTTTTGAGCTTTATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCatcttg                    <  1:80051/140‑1 (MQ=255)
                                                                                                               tACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTGTTTAGGTTCatcttgc                   >  1:554966/1‑139 (MQ=255)
                                                                                                               tACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACATGATGTTACAGAACAAATAGAGGATACTTTTTTACAATTATGATGTTTTTTAGGTTCatcttga                   >  1:231882/1‑140 (MQ=255)
                                                                                                                 cTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAATCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCatcttgatc                 <  1:664363/140‑1 (MQ=255)
                                                                                                                 cTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGTTGCTTTTTTACCATTATGATGTTCTTTAGGTTCatcttgatc                 <  1:543291/140‑1 (MQ=255)
                                                                                                                 cTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATCATGTTCTTTAGGTTCatcttgatc                 >  1:254234/1‑140 (MQ=255)
                                                                                                                    ttgttttgAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCGGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCatcttgatcttg              >  1:485414/1‑140 (MQ=255)
                                                                                                                     tgttttgAGCGTCGTTTGAATGTTTCTTAGCTGTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGt             <  2:668396/140‑1 (MQ=255)
                                                                                                                     tgttttgAGCGTCATTTGGATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGt             <  1:536322/140‑1 (MQ=255)
                                                                                                                     tgttttgAGCGTCATTTGGATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGt             <  1:536305/140‑1 (MQ=255)
                                                                                                                      gttttgAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGtt            >  2:580666/1‑140 (MQ=255)
                                                                                                                       ttttgAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGttt           <  1:88562/140‑1 (MQ=255)
                                                                                                                       ttttgAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGttt           <  1:153704/140‑1 (MQ=255)
                                                                                                                       tttcgtgcttcttttGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGTAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGttt           <  2:147581/128‑1 (MQ=39)
                                                                                                                        tttgAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCACTAGCTGCTGCTTTTTTACCATTATGATGTTCTTTTGGTTCATCTTGATCTTGtttt          <  2:335726/140‑1 (MQ=255)
                                                                                                                          tgAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACGATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTAc        <  2:364153/140‑1 (MQ=255)
                                                                                                                           gAGCGTCATTTGGATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACa       >  2:727733/1‑140 (MQ=255)
                                                                                                                             gCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCATCAGCTGCACCTGAAACATCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGa     <  1:418755/140‑1 (MQ=255)
                                                                                                                              cGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGaa    <  1:587193/140‑1 (MQ=255)
                                                                                                                                acATTTGAACGTTTCTTAGCTTTAGAAGCAGCCATTGCAAGAGATGCACCTGCAACACCTGATGTTCCAGAACCAATAACTGCTACCTTTTTCACATTATGATGTTCTTTCGGTTCATCTTGATCTCGTTTTACAGCAta  >  2:268241/2‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CCAAACCTGCTGTTCCAGCACCGATCGCTGCACCTTTTTTGCCATTATGATGATCTTTAGACTTATCTTGAGACGCTTTATCCTCAGTCGAGTTATTCGCCTTGCCAGAATTACTTTTGTTTTGAGCGTCATTTGAATGTTTCTTAGCTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATC  >  CP000730/1551648‑1551915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑