Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,516 C→A 32.8% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5160CA32.8% 74.9 / 55.0 76Q196K (CAA→AAA) USA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base A (10/15);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

TGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAA  >  CP000730/111377‑111649
                                                                                                                                           |                                                                                                                                     
tGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                       <  2:427190/140‑1 (MQ=255)
tGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                       <  2:218750/140‑1 (MQ=255)
   agAAGTTAAAAAAAAAAACATAAAAAAAAAAATCGAAAACTATAAGTTTTTTGCGCAATATAGCAATTTTAAAGAATTAAGGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  1:64018/139‑1 (MQ=17)
    gAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                   <  2:611753/140‑1 (MQ=255)
     aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                                                  >  1:393678/1‑140 (MQ=255)
       gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGACGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                                <  1:646586/140‑1 (MQ=255)
        ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTAAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGAGTTATTAAGCTCAATATCac                                                                                                                               >  2:475107/1‑139 (MQ=255)
            agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                           <  1:30120/140‑1 (MQ=255)
            agaaaaAAAAAGAAAAAAAGAAATCGAAAAGTTTAAGTTTTTTGCGGAAGAGAACAAGTTCAAAGAGTTAATTAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTGATTCGGCTAAATATCAATTaa                                                                                                                           <  2:211249/140‑1 (MQ=17)
             gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                          >  2:69643/1‑140 (MQ=255)
             gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAACTTTTTGCGCAATACAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATACCATATAATCCAGAGGTCCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                          >  2:589343/1‑140 (MQ=21)
               agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                                                        <  1:3237/140‑1 (MQ=255)
               agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                                                        <  1:261914/140‑1 (MQ=255)
               agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTa                                                                                                                        >  2:190929/1‑140 (MQ=255)
                gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCAAAATATAAATTAACTaa                                                                                                                       >  2:26519/1‑140 (MQ=255)
                gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                                                       >  1:328228/1‑140 (MQ=255)
                   aaCATAAAAAAAGAAATCGAAAACATTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATCATACCGAGGTGCCGAGTTATACGGCTAAATATCAATTAACTAatga                                                                                                                    >  2:151286/1‑140 (MQ=18)
                    aaaTAAAAAAATAAATCGATAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                   <  1:157450/138‑1 (MQ=255)
                    aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATCGACATGTTTAAGATGTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTCTTCGGCTAAATATCAATTAACTAatgat                                                                                                                   >  2:697947/1‑140 (MQ=14)
                    aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                   >  1:637115/1‑140 (MQ=255)
                    aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                   >  2:378464/1‑140 (MQ=255)
                    aCATAAAAAAAGAAATCGAAAACTGTAAGTTTTTTGCGCAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATGATATAATACAGGTGTAGCGAGTTATTCGGCTAAATATCAATTAACTAatggt                                                                                                                   >  2:329573/1‑138 (MQ=17)
                      aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                 <  2:656636/140‑1 (MQ=255)
                       tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATTAATTAACTAATgatgat                                                                                                                >  1:154000/1‑140 (MQ=255)
                        aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                               <  2:674695/140‑1 (MQ=255)
                          aaaaaGAAATCTAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                             <  1:686695/140‑1 (MQ=255)
                          aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATGATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                             <  1:154713/140‑1 (MQ=255)
                             aataaagccaaaaCTTTAATTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                                                          <  1:161266/131‑1 (MQ=17)
                               gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGAAATCTTAAAGATTTAATGAATTATAATGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGt                                                                                                        <  1:491478/140‑1 (MQ=255)
                                aaaTCGAAAACTTTAAGTTTTTTACGCAATATAGCAATTTTAAAGATTTAATGAATTAGAAAGATGGAGATATATCATATAATCCATAGGTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTa                                                                                                       <  2:41700/140‑1 (MQ=255)
                                 aaTCGAACACTTTAAGTTTTTTGGGGAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:507742/140‑1 (MQ=255)
                                 aaTCGAAAACTTTAAGTTTTTTGCTCAATATAGCAATTTTAAAGATTTAATAAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGACGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      >  2:587002/1‑140 (MQ=255)
                                 aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAAGATGATTATAATGTaa                                                                                                      >  1:153753/1‑140 (MQ=255)
                                 aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:676522/140‑1 (MQ=255)
                                 aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:372350/140‑1 (MQ=255)
                                 aaTCAAAAACTTTAGTTTTGATGAGAAATATAGCAATTTTAAAGATTTAATGAATGATAAAGATGGAGATATATCATATAATCCAGAGGTGCGGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:658367/140‑1 (MQ=18)
                                  aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGGTTATAATGTaaa                                                                                                     >  2:247139/1‑140 (MQ=255)
                                  aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                     >  1:706045/1‑140 (MQ=255)
                                   tCGAGAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGTGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    <  2:529003/140‑1 (MQ=18)
                                   tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    <  2:698098/140‑1 (MQ=255)
                                   tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCACAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    >  1:335543/1‑140 (MQ=255)
                                   tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATAATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    >  2:159456/1‑140 (MQ=255)
                                       aaaCTTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                <  1:276314/140‑1 (MQ=255)
                                       aaaCTTTAAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                <  1:614723/140‑1 (MQ=255)
                                          cTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                             <  1:98595/140‑1 (MQ=255)
                                              aaGTTTTTTGCTCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                         <  1:190929/140‑1 (MQ=255)
                                              aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                         >  2:398837/1‑140 (MQ=255)
                                              aaGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                         <  1:97154/140‑1 (MQ=255)
                                               aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaa                                                                                        >  1:666693/1‑140 (MQ=255)
                                                       gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGATATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGAAAAACAATTACGTAAAAGatataa                                                                                >  1:487511/1‑138 (MQ=255)
                                                       gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATAGGa                                                                                >  1:610777/1‑140 (MQ=255)
                                                            atatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAc                                                                           >  2:397488/1‑140 (MQ=255)
                                                             tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAcc                                                                          >  2:599751/1‑140 (MQ=255)
                                                               tcgCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATAGAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                        <  2:389283/138‑3 (MQ=11)
                                                                      tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATAATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaa                                                                 >  1:550683/1‑140 (MQ=255)
                                                                      ttaaacgATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaa                                                                 <  2:384589/134‑1 (MQ=255)
                                                                            gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                           <  2:538574/140‑1 (MQ=255)
                                                                            gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTCATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGAGcc                                                           >  1:470542/1‑140 (MQ=255)
                                                                                  aTGAATTATAAAGATGGAGATATCTTGACGAAGCCATAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAAATGAAATACCAACAAATAAAGCGACGAAGCt                                                     >  1:172920/1‑140 (MQ=17)
                                                                                   tGAATTATAAAGATTGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                    <  2:513770/140‑1 (MQ=255)
                                                                                      aTTATAAAGATGGATACTTACCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTACAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                 <  1:636107/140‑1 (MQ=14)
                                                                                        tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTgttgtt                                               <  2:505298/140‑1 (MQ=255)
                                                                                                    gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                   <  1:378464/140‑1 (MQ=255)
                                                                                                      tatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCTGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCTGCGAAGATGTTGTTGAAAGGTACAGGGa                                 >  2:590323/1‑140 (MQ=255)
                                                                                                          tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGAAttt                             >  2:238481/1‑140 (MQ=255)
                                                                                                                 aTCCAGAGGTGCCAAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                      <  1:449343/140‑1 (MQ=255)
                                                                                                                   cctgagGTGCCGAGTTATTCAGCTCAATAACCATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATTACATACCAACAAAGAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                    <  1:115183/137‑1 (MQ=17)
                                                                                                                   ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                    <  2:704754/140‑1 (MQ=255)
                                                                                                                    cAGATGTGACGAGTTATTCAGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAACGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                   <  1:246021/140‑1 (MQ=255)
                                                                                                                    cAGAGGTGCCGAGTTATACATCTCAAGATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                   <  1:14088/140‑1 (MQ=255)
                                                                                                                      gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGCTTATAATGTAAAACAATTACGTAAAAGATTTGCCATACCAACAACTAAAGCGCCGAAGATGTTCTTGAAAGGTACAGGCAATTAAAAAGAttactc                 >  1:596776/1‑136 (MQ=14)
                                                                                                                        ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGAGTATAATGTAAAACAATTACGTGAAAGATATGACATACCAACAACTAAAACGCCGAAGCTGTTGTTGAAAGGTACAGCGAATTTAAAAAGTTCATCAg               >  1:230037/1‑140 (MQ=17)
                                                                                                                               aGTTATTCAGCTCAATATCAATTAACTAATTATGATTATAATGTAAAACAATTATGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGTTTCATCAGTTGGata        <  2:271393/140‑1 (MQ=255)
                                                                                                                                  tataCAGCTCAAAAAGAATGGACTAAGGATGATTATAAGGTAAATCAGGAACGGAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaa     >  2:719225/1‑140 (MQ=14)
                                                                                                                                   aTTCAGCTCAATGTCAATTAACAAATGATAATTATAATGTAAAACAATTACGTAAAAGACATGACATAGCTACTAATAAAGCGCCGAAGCTGTTGTTGAAAAGTAGAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa    <  2:459651/140‑1 (MQ=17)
                                                                                                                                   aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa    <  1:575460/140‑1 (MQ=255)
                                                                                                                                     tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa  >  2:348057/1‑140 (MQ=255)
                                                                                                                                     tCAGCTCAATATCAATTAACTAATAATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa  >  1:464136/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                     
TGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAA  >  CP000730/111377‑111649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: