Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 367,644 G→T 100% intergenic (‑96/‑20) nanA ← / → nagC N‑acetylneuraminate lyase/possible transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730367,6440GT100.0% 196.6 / ‑6.5 65intergenic (‑96/‑20)nanA/nagCN‑acetylneuraminate lyase/possible transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (39/25);  total (39/26)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATG  >  CP000730/367510‑367777
                                                                                                                                      |                                                                                                                                     
gAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCata                                                                                                                                  >  2:572733/1‑140 (MQ=255)
    aaCGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTa                                                                                                                              <  1:207367/140‑1 (MQ=255)
     aCGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTAt                                                                                                                             >  2:511347/1‑140 (MQ=255)
     aCGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTAt                                                                                                                             >  2:377866/1‑140 (MQ=255)
      cGCTTCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATg                                                                                                                            <  2:315074/140‑1 (MQ=255)
       gCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAACAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGt                                                                                                                           <  1:711806/140‑1 (MQ=255)
            atatAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaat                                                                                                                      <  1:654384/140‑1 (MQ=255)
                aaaCCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaattaat                                                                                                                  >  2:590087/1‑140 (MQ=255)
                  aCCTTTTAAATATTTGTTCATTATGAAGTCCTGCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGa                                                                                                                <  1:130995/140‑1 (MQ=255)
                    cTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTTAATATTTTATTCATATTTATGTATAATTAATGaaa                                                                                                              >  2:677567/1‑140 (MQ=255)
                    cTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATATATAATTAATGaaa                                                                                                              >  2:289602/1‑140 (MQ=255)
                         aaaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTTTGTATAATTAATGAAAATGtt                                                                                                         >  2:661165/1‑140 (MQ=255)
                         aaaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGtt                                                                                                         >  2:106841/1‑140 (MQ=255)
                          aaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGttt                                                                                                        >  1:176522/1‑140 (MQ=255)
                          aaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAAAGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATtttt                                                                                                        >  1:209029/1‑140 (MQ=255)
                            tCTTTGTTCATGATGAAGTCCCTCCAAAAATTATTTTATAATAGTGTTCATAGCTATAATTGTAAACGATATCATATAATGTAACAATATCTTTTTGAAAATTGTATGCATATTTATGTATAATTAATGAAAATGttttt                                                                                                      <  2:234562/140‑1 (MQ=255)
                                tGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCaaa                                                                                                  >  2:344100/1‑140 (MQ=255)
                                    cATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCa                                                                                              >  2:383975/1‑140 (MQ=255)
                                    cATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCa                                                                                              >  2:604292/1‑140 (MQ=255)
                                     aTTATGAAGTCCCCCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCaa                                                                                             <  1:702777/140‑1 (MQ=255)
                                      ttATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAAt                                                                                            <  2:119342/140‑1 (MQ=255)
                                       tATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACGGCTATAATTGTAAACGCTACCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATa                                                                                           >  2:81089/1‑140 (MQ=255)
                                       tATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATAAAAATGTTTTTCAAAATCAATa                                                                                           >  1:12677/1‑140 (MQ=255)
                                          gAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaa                                                                                        >  1:145027/1‑140 (MQ=255)
                                           aaGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTAGAATTGTAAACGCTATCATAAAATGTAACAATATTTTTTTGAAAATTGTATTCATATGTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaaa                                                                                       <  2:107063/140‑1 (MQ=255)
                                             gTCCCACCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATg                                                                                     <  1:139537/140‑1 (MQ=255)
                                                 cTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGATATCATAACATGTAATAATATCTTTTTGAAAATTGTATTAATATTTATGTATAATTAAAGAACATATTTTTCAAAATCAATAGAAATGgatt                                                                                 >  2:630338/1‑138 (MQ=255)
                                                  tCCAAAAATCATTTGATAATATTGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtg                                                                                <  1:464035/140‑1 (MQ=255)
                                                  tCCAAAAATCATTTGATAATATATTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtg                                                                                <  1:660486/140‑1 (MQ=255)
                                                  tCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtg                                                                                >  2:30099/1‑140 (MQ=255)
                                                  tCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtg                                                                                <  1:544299/140‑1 (MQ=255)
                                                  tCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGGATTCATAATTAAGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagag                                                                                >  2:365799/1‑138 (MQ=255)
                                                   ccAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAACCGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAACATCAATAAAAATGgagtga                                                                               >  2:76362/1‑140 (MQ=255)
                                                    taacaaTCATTTTATAATATATGTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATATTAGGCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtgag                                                                              <  1:457792/136‑1 (MQ=255)
                                                         aTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAgg                                                                         <  1:655050/140‑1 (MQ=255)
                                                          tCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAGTGTTTTTCAAAATCAATAGAAATGGAGTGATAAAGgt                                                                        >  2:230809/1‑140 (MQ=255)
                                                          tCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgt                                                                        >  1:402330/1‑140 (MQ=255)
                                                                   aataTAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGAAAGGTGTATTAAAt                                                               >  1:594704/1‑140 (MQ=255)
                                                                      ataGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGc                                                            >  1:650871/1‑140 (MQ=255)
                                                                       taGTTTACAGCTATAATTGTAAACGCTGTCATAAAATGTAACAATATCTTTTTGGAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATACAAATGGAGTAAGTAAGGTGTATTACATCGCa                                                           >  1:485546/1‑140 (MQ=255)
                                                                         gTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAAt                                                         >  1:508609/1‑140 (MQ=255)
                                                                             aCAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGat                                                     >  1:569346/1‑140 (MQ=255)
                                                                                gCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGagaat                                                  >  2:614991/1‑136 (MQ=255)
                                                                                gCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATAtt                                                  >  2:338584/1‑140 (MQ=255)
                                                                                gCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGCAAGGTGTATTACATCGCAATCGATAtt                                                  >  2:726642/1‑140 (MQ=255)
                                                                                       ttGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCa                                           >  2:264200/1‑140 (MQ=255)
                                                                                          tAAACGCTATCATAAAAAGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGGGTGAGAAAGGTGTATTACATTGCAATCGCTGTTggtgacccat                                        >  2:106827/1‑132 (MQ=255)
                                                                                           aaaCGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCa                                       <  1:590087/140‑1 (MQ=255)
                                                                                           aaaCGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGAACTCa                                       >  2:288949/1‑140 (MQ=255)
                                                                                           aaaCGCTATCATAAAATGTAACAATATCTTTCTGAAAATTGTATTCATATTTATGGATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCa                                       <  1:486570/140‑1 (MQ=255)
                                                                                             aCGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCaaa                                     <  1:110771/140‑1 (MQ=255)
                                                                                                  aTCATAAAATGTAACAATATCTTTTTGAACATTGTTTTCATTAGGAGGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTaaa                                <  1:377866/140‑1 (MQ=255)
                                                                                                    cATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATc                              <  2:145027/140‑1 (MQ=255)
                                                                                                       aaaaTGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGc                           >  1:654952/1‑140 (MQ=255)
                                                                                                          aTGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTTAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGt                        <  1:604292/140‑1 (MQ=255)
                                                                                                          aTGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATAAAATCGGCAga                        >  1:658461/1‑139 (MQ=255)
                                                                                                          aTGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATAACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGt                        <  1:383975/140‑1 (MQ=255)
                                                                                                              aaCAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTAtt                    >  1:278280/1‑140 (MQ=255)
                                                                                                                aattgtcgttgttaaaaTTGTATTTATATTTATTTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGGAAGGGGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGa                  <  1:309286/127‑1 (MQ=255)
                                                                                                                  atctCTTTTGGAAAAGTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCTTTCGATATTGGTGGCACTCAAATTAAATCGGCAGTTTTTGATa                <  2:572401/137‑1 (MQ=255)
                                                                                                                      cTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCa            <  1:511347/140‑1 (MQ=255)
                                                                                                                            gAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGaa      <  1:30099/140‑1 (MQ=255)
                                                                                                                             aaaaTTGTATTTATATTTATGTATCATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGAAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAat     >  1:226237/1‑140 (MQ=255)
                                                                                                                                aTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAAGATg  >  1:55167/1‑140 (MQ=255)
                                                                                                                                aTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAAAATg  >  2:417567/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATG  >  CP000730/367510‑367777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑