Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	454,656	G→A	V119I (GTT→ATT)	set23 →	staphylococcal exotoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	454,656	0	G	A	98.6%	227.4 / ‑4.4	69	V119I (GTT→ATT)	set23	staphylococcal exotoxin
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (39/29);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

TTGCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTGTTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAGTGTC  >  CP000730/454518‑454792                                                                                                                                           |                                                                                                                                         ttGCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATttagtatt                                                                                                                                        >  1:109320/1‑141 (MQ=255)   gCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATttagtattag                                                                                                                                      <  1:812697/141‑1 (MQ=255)    cTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATttagtattagt                                                                                                                                     <  1:200195/141‑1 (MQ=255)           aCCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTaa                                                                                                                              <  1:777090/141‑1 (MQ=255)            ccAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATTGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAAc                                                                                                                             >  1:825949/1‑141 (MQ=255)            ccAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGATTAGATGTCTTTTCGGTAACAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAAc                                                                                                                             >  1:161573/1‑141 (MQ=255)             caacaaAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACa                                                                                                                            <  2:343436/141‑1 (MQ=255)             caacaaAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACa                                                                                                                            <  1:126992/141‑1 (MQ=255)             caacaaAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAACACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACa                                                                                                                            >  2:398865/1‑141 (MQ=255)              aacaaAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACaa                                                                                                                           <  1:308353/141‑1 (MQ=255)              aacaaAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCATTGCGGTACCAGAATTAATAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACaa                                                                                                                           >  2:824807/1‑141 (MQ=255)               acaaAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACaaa                                                                                                                          >  1:536636/1‑141 (MQ=255)                ttttcttaaaaGTTAAAAGTGTTTTTATTGGGAACCGATGACAATAACTAAAAAGAAAAAACACATGGTTTAGATGTCTTTGTGGTACCAGAATTAGTAGATTTAGATGGAAGAATCTTTAGTATTAGTGGTGTATCAAAg                                                                                                                         >  2:728390/8‑141 (MQ=255)                    aTCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTATCAAAGaaaa                                                                                                                     <  1:761403/141‑1 (MQ=255)                    aTCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGaaaa                                                                                                                     <  1:619532/141‑1 (MQ=255)                    aTCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGaaaa                                                                                                                     <  2:339951/141‑1 (MQ=255)                      cAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAAc                                                                                                                   <  1:549569/141‑1 (MQ=255)                        aaaGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGt                                                                                                                 >  1:368162/1‑141 (MQ=255)                         aaGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTa                                                                                                                >  1:176556/1‑141 (MQ=255)                            ttCCAAGTGTTTTTATAGGGAAAAGATGAAAATAAATACAAAGAATAAACACATGGGTTAGATGGCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAAAATTTAGTATTAGTAGTGTAACAAAGAAAAACGTaaaa                                                                                                             >  1:117617/1‑141 (MQ=255)                                aaGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCaa                                                                                                         <  1:112563/141‑1 (MQ=255)                                aaGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCaa                                                                                                         >  2:431232/1‑141 (MQ=255)                                  gtgtTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAata                                                                                                       >  1:137238/1‑141 (MQ=255)                                       tttATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGa                                                                                                  >  1:478464/1‑141 (MQ=255)                                       tttATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCCTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTTGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGa                                                                                                  <  1:625930/141‑1 (MQ=255)                                        ttATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAg                                                                                                 >  2:13452/1‑141 (MQ=255)                                         tATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGt                                                                                                >  2:819358/1‑141 (MQ=255)                                           ttGGGAAAAGAGGAAAATAAAAACAAAAAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGtct                                                                                              <  2:822511/141‑1 (MQ=255)                                            tGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGtctc                                                                                             <  2:587404/141‑1 (MQ=255)                                            tGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGtctc                                                                                             >  1:432061/1‑141 (MQ=255)                                              ggAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTa                                                                                           >  1:243646/1‑141 (MQ=255)                                                  aaGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGaa                                                                                       <  2:357401/141‑1 (MQ=255)                                                   aGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAAc                                                                                      >  1:223323/1‑141 (MQ=255)                                                         aaataaatACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTGTTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGaa                                                                                >  2:518617/1‑141 (MQ=255)                                                             aaataCAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTa                                                                            <  2:394064/141‑1 (MQ=255)                                                              aataCAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAACACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTAc                                                                           >  1:126216/1‑141 (MQ=255)                                                              aataCAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTAc                                                                           >  2:530945/1‑141 (MQ=255)                                                              aataCAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTAc                                                                           <  1:13452/141‑1 (MQ=255)                                                                taCAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTa                                                                         >  1:781832/1‑141 (MQ=255)                                                                taCAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTa                                                                         >  2:108329/1‑141 (MQ=255)                                                                 aCAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAg                                                                        <  2:814647/141‑1 (MQ=255)                                                                   aaaGAAAAAAAAAATAGTTTAGATGTATTTGCGGTACAAGAATTAATAAAATAAAAAAGAAAAATATTTAGTATTAGAGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTTATATCGCTGaaaaaaaaaaaa                                                                      <  2:740549/141‑11 (MQ=255)                                                                            acacATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATa                                                             >  2:386590/1‑141 (MQ=255)                                                                              acaTGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGa                                                           >  1:270251/1‑141 (MQ=255)                                                                                      tAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATGTTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAg                                                   >  1:387383/1‑141 (MQ=255)                                                                                         aTGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTGACTAGTTAAAAAAATAGACGATAAAGACg                                                >  1:421834/1‑141 (MQ=255)                                                                                          tGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACgg                                               <  1:398865/141‑1 (MQ=255)                                                                                            tCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATCTTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGtt                                             >  1:774535/1‑141 (MQ=255)                                                                                             cTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGttt                                            <  2:672640/141‑1 (MQ=255)                                                                                             cTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGttt                                            <  1:124246/141‑1 (MQ=255)                                                                                              tttGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGtttt                                           >  2:1216/1‑141 (MQ=255)                                                                                                  cGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTGCTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTa                                       >  1:682490/1‑141 (MQ=255)                                                                                                    gTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTAtt                                     >  2:266021/1‑141 (MQ=255)                                                                                                    gTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAAGCAATATTGGAGGCGCTAAGAACGCCGAACGTAGTAGTTGAAAAAATAGACGATAAAGACGGTTTTTCTAtt                                     >  1:517306/1‑141 (MQ=255)                                                                                                     tACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATCCCGACGGTTTTTCTATtg                                    >  1:697086/1‑141 (MQ=255)                                                                                                      aCCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATtga                                   >  2:680183/1‑141 (MQ=255)                                                                                                       ccAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATtgat                                  >  2:845043/1‑141 (MQ=255)                                                                                                       ccAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATtgat                                  <  2:769533/141‑1 (MQ=255)                                                                                                        cAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATtgatg                                 <  2:302944/141‑1 (MQ=255)                                                                                                        cAGAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATtgatg                                 <  2:554178/141‑1 (MQ=255)                                                                                                        aaGAATTAGTAGATTTAAATGAAAGAATATTTAGTATTAGTGGTATAAAAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATtgatg                                 <  2:417710/140‑1 (MQ=255)                                                                                                          gAATTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGaa                               >  1:791190/1‑141 (MQ=255)                                                                                                            aTTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTTTATTTATGAttt                             >  2:386917/1‑141 (MQ=255)                                                                                                            aTTAGTAGATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAAtt                             <  2:243646/141‑1 (MQ=255)                                                                                                                 tagATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCt                        <  2:796442/141‑1 (MQ=255)                                                                                                                   gATTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCttt                      <  1:156425/141‑1 (MQ=255)                                                                                                                    aTTTAGATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTa                     >  1:312892/1‑141 (MQ=255)                                                                                                                         gATGGAAGAATATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCaa                <  2:478464/141‑1 (MQ=255)                                                                                                                                gaaTATTTAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGgaag         <  1:354694/141‑1 (MQ=255)                                                                                                                                     tttAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAgtg    >  2:224504/1‑141 (MQ=255)                                                                                                                                     tttAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAgtg    >  2:261628/1‑141 (MQ=255)                                                                                                                                     tttAGTATTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAgtg    >  1:708959/1‑141 (MQ=255)                                                                                                                                      ttagtattagtgGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAgtgt   >  1:677823/1‑141 (MQ=255)                                                                                                                                       tagtattagtgGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAgtgtt  >  2:794702/1‑140 (MQ=255)                                                                                                                                       tagtattagtgGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAGTGTc  >  2:675117/1‑141 (MQ=255)                                                                                                                                       tagtattagtgGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGGTTTTCTATTGAGGAATTTTTCTGTATTCAAAAGGAAGAAGTGTc  >  2:561109/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                         TTGCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACAAAGAAAAAACACATGGTTTAGATGTCTTTGCGGTACCAGAATTAGTAGATTTAGATGGAAGAATATTTAGTGTTAGTGGTGTAACAAAGAAAAACGTAAAATCAATATTTGAGTCTCTAAGAACGCCGAACTTACTAGTTAAAAAAATAGACGATAAAGACGGTTTTTCTATTGATGAATTTTTCTTTATTCAAAAGGAAGAAGTGTC  >  CP000730/454518‑454792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑