Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,232,465	G→C	A124P (GCG→CCG)	prkC →	non‑specific serine/threonine protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,232,465	0	G	C	100.0%	120.0 / NA	35	A124P (GCG→CCG)	prkC	non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/17);  total (18/17)

AGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATC  >  CP000730/1232332‑1232597                                                                                                                                      |                                                                                                                                     agaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGa                                                                                                                               >  2:239099/1‑141 (MQ=255)  gaTGACTGTTACTACTTAGTAATGGAATATAGCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATAAAGTGTTGACACAGCGATTAATTTTACGACTCAAATATTGGAGGGCATTCAACATCAGCATAat                                                                                                                              >  2:318617/1‑141 (MQ=255)    tGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGatat                                                                                                                            <  2:320831/141‑1 (MQ=255)      agttttACTACTTAGTAATGTAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGc                                                                                                                          <  1:119418/137‑1 (MQ=255)        tGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGt                                                                                                                        <  1:188597/141‑1 (MQ=255)                    gTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATaga                                                                                                            <  1:103114/141‑1 (MQ=255)                          gAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATAtt                                                                                                      >  2:248316/1‑141 (MQ=255)                          gAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATAtt                                                                                                      >  2:248296/1‑141 (MQ=255)                          gAATATATCGAAGGTCAGAATTTGTCTGAGTATATTGAAAGACATGGTGCATTAAGTGTTTATACAACGATTTATATTATGAATGAAGTCTTGGATGGCATTAAATATCTGCATGATACGCGTATTGTGCATAGAGATAtt                                                                                                      >  2:248333/1‑141 (MQ=255)                            atatatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTaa                                                                                                    >  1:62333/1‑141 (MQ=255)                                  cGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcaca                                                                                              >  1:299956/1‑141 (MQ=255)                                   gAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACaa                                                                                             >  1:168999/1‑141 (MQ=255)                                          cGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatatat                                                                                      <  2:62333/141‑1 (MQ=255)                                           gACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATAtt                                                                                     <  2:90812/141‑1 (MQ=255)                                                     gAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGc                                                                           <  2:99137/141‑1 (MQ=255)                                                       gTATATTGAAAGTCATGGGCCATTAAGTCTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCaa                                                                         <  1:326299/141‑1 (MQ=255)                                                         atatTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATa                                                                       <  1:230207/141‑1 (MQ=255)                                                           atTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTAAGAATCAAATATTGGATGGAATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAACTATATTAATTGACAGCAATaaa                                                                     >  2:238347/1‑141 (MQ=255)                                                             tGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAAc                                                                   >  1:188486/1‑141 (MQ=255)                                                                 aGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTg                                                               <  1:64806/141‑1 (MQ=255)                                                                    cATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaa                                                            >  1:165776/1‑141 (MQ=255)                                                                     aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATTATATGCGTAGTGTACAGAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaaa                                                           >  1:276293/1‑141 (MQ=255)                                                                     aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaaa                                                           >  1:192156/1‑141 (MQ=255)                                                                       gggCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAata                                                         <  1:269579/141‑1 (MQ=255)                                                                                   gtTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGa                                             >  1:49128/1‑141 (MQ=255)                                                                                        cacaGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGc                                        <  1:314923/141‑1 (MQ=255)                                                                                               aTTAATTTTACGAATCAAATATTGGATGGCATTAAACA‑CCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCtt                                >  2:358541/1‑141 (MQ=255)                                                                                                       tACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTga                         <  1:37310/141‑1 (MQ=255)                                                                                                       tACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTga                         <  2:280767/141‑1 (MQ=255)                                                                                                       tACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTga                         <  2:195607/141‑1 (MQ=255)                                                                                                            ataaaaTATAGGATGGCATGAAACATCCGCATGAGATGCGTATTGTACAGAGAGAAATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGt                    <  2:89244/138‑1 (MQ=255)                                                                                                                     tGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTc           >  1:37016/1‑141 (MQ=255)                                                                                                                         tGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGAc       >  1:257882/1‑141 (MQ=255)                                                                                                                              ttAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATc  >  2:60892/1‑141 (MQ=255)                                                                                                                              ttAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATc  <  2:199747/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     AGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATC  >  CP000730/1232332‑1232597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑