Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,289,325	C→A	Q55H (CAG→CAT)	ybbP ←	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,289,325	0	C	A	100.0%	103.2 / NA	31	Q55H (CAG→CAT)	ybbP	hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/22);  total (9/22)

AGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTACTGA  >  CP000730/2289203‑2289461                                                                                                                           |                                                                                                                                         aGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACt                                                                                                                        <  2:104520/141‑1 (MQ=255)   ttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTaa                                                                                                                      >  1:268343/1‑141 (MQ=255)     gttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAata                                                                                                                    <  1:231738/141‑1 (MQ=255)     gttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAata                                                                                                                    <  1:231742/141‑1 (MQ=255)               aCGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAg                                                                                                          <  1:290553/141‑1 (MQ=255)                          cTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCg                                                                                               <  2:148928/141‑1 (MQ=255)                           tGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                                                                                              <  2:74710/141‑1 (MQ=255)                           tGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                                                                                              <  2:278509/141‑1 (MQ=255)                            ggTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCt                                                                                             >  2:47476/1‑141 (MQ=255)                              ttGGAATATTACTATTAAAGCTAATACACACCATTGAATATCGATATATTATAATTTAGATGTTGTAGTAAAGTTAAATATCATATTTATCTGATGACAAATAACAATTACTAATATCCGTTTAGGTAATTGTATCGCttt                                                                                           >  2:262574/1‑141 (MQ=255)                               tGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTa                                                                                          <  2:63906/141‑1 (MQ=255)                               tGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTa                                                                                          >  2:306807/1‑141 (MQ=255)                                    tatTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTcc                                                                                     <  2:35946/141‑1 (MQ=255)                                      ttACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCt                                                                                   <  2:86533/141‑1 (MQ=255)                                       tACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCtt                                                                                  >  2:342389/1‑141 (MQ=255)                                         cTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTaa                                                                                <  2:311927/141‑1 (MQ=255)                                                aGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGt                                                                         >  2:53831/1‑141 (MQ=255)                                                 gCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtg                                                                        <  1:299661/141‑1 (MQ=255)                                                 gCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtg                                                                        <  2:226309/141‑1 (MQ=255)                                                      tACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgag                                                                   <  2:309254/141‑1 (MQ=255)                                                          ccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGagaaga                                                               <  1:229365/141‑1 (MQ=255)                                                           cccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGAt                                                              <  1:260399/141‑1 (MQ=255)                                                            ccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATa                                                             <  1:227788/141‑1 (MQ=255)                                                              aTTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATaaa                                                           <  2:327186/141‑1 (MQ=255)                                                                 gAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTa                                                        <  1:28242/141‑1 (MQ=255)                                                                    tAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACAt                                                     >  2:30747/1‑141 (MQ=255)                                                                       cGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATAcc                                                  <  2:359633/141‑1 (MQ=255)                                                                         atatCGAATAATATAGATGCTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCaa                                                <  2:257072/141‑1 (MQ=255)                                                                              gAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTAt                                           >  2:98522/1‑141 (MQ=255)                                                                                                                       tCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTACTGa  <  1:302484/141‑1 (MQ=255)                                                                                                                       tCTGATGAACAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTGCTGa  >  2:217844/1‑141 (MQ=255)                                                                                                                           |                                                                                                                                         AGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTACTGA  >  CP000730/2289203‑2289461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑