Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,502,141 C→A A222D (GCC→GAC)  USA300HOU_2364 → AraC transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,502,1410CA97.1% 104.6 / NA 35A222D (GCC→GAC) USA300HOU_2364AraC transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (18/16);  minor base T (1/0);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATCA  >  CP000730/2502004‑2502274
                                                                                                                                         |                                                                                                                                     
tGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACgg                                                                                                                                    <  2:267170/141‑1 (MQ=255)
tGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACgg                                                                                                                                    >  1:282041/1‑141 (MQ=255)
       tatTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAAc                                                                                                                             >  2:88577/1‑141 (MQ=255)
           gTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTc                                                                                                                         >  1:55966/1‑141 (MQ=255)
           gTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTc                                                                                                                         >  1:718/1‑141 (MQ=255)
           gTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTGTCTACAAAACATTCTATTACAACTGTTTCTGAGTTGGACGGTTTTAACAGTc                                                                                                                         >  1:23387/1‑141 (MQ=255)
             tCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCAt                                                                                                                       <  2:25434/141‑1 (MQ=255)
              cTAATCTTTTTTTTTTTTTTTTTAGTATGAAATTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTTTTACTTTCTACAATACATTCTATTTCTTCTTTTTCTTATTTTTTCGGTTTTAACAGTCATa                                                                                                                      >  2:177866/1‑141 (MQ=255)
                 aTCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTATTAACAGTCATACaa                                                                                                                   <  2:166573/141‑1 (MQ=255)
                                   tGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTa                                                                                                 >  1:363305/1‑141 (MQ=255)
                                     aGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAg                                                                                               >  1:67890/1‑141 (MQ=255)
                                            aTTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACt                                                                                        <  1:181812/141‑1 (MQ=255)
                                                      ttattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAg                                                                              <  2:15738/141‑1 (MQ=255)
                                                      ttattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAg                                                                              >  1:243361/1‑141 (MQ=255)
                                                       tattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGt                                                                             >  1:15643/1‑141 (MQ=255)
                                                       tattTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGt                                                                             <  1:28688/141‑1 (MQ=255)
                                                         ttttACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTcc                                                                           <  1:206063/141‑1 (MQ=255)
                                                                        aCTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTTCATTTTAGTCCTAAACAGTTCCGTTc                                                            >  2:337009/1‑141 (MQ=255)
                                                                          ttGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCAc                                                          <  1:175210/141‑1 (MQ=255)
                                                                           tGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACt                                                         >  2:21877/1‑141 (MQ=255)
                                                                             ttAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCg                                                       >  1:195376/1‑141 (MQ=255)
                                                                                       cAATTTATTACTTTCTACAAAACCTTCTATTACTACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATGACTTGCATGTTAGTCCTAAACAGTTTCGTTCACTCGTTTCCAAGAt                                             >  2:238511/1‑141 (MQ=255)
                                                                                         aTTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTa                                           <  2:11066/141‑1 (MQ=255)
                                                                                            tattaCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTg                                        >  1:145789/1‑141 (MQ=255)
                                                                                            tattaCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTg                                        >  1:198888/1‑141 (MQ=255)
                                                                                            tattaCTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTg                                        <  1:55275/141‑1 (MQ=255)
                                                                                                   ttCTACAAAACATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAaccacc                                 <  1:337446/141‑1 (MQ=255)
                                                                                                           aaCATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATac                         >  2:249796/1‑141 (MQ=255)
                                                                                                           aaCATTCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATac                         <  1:365055/141‑1 (MQ=255)
                                                                                                               ttCTATTACAACTGTTTCTGAATTGGACGGTTTTAACTTTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACtt                     >  2:172974/1‑141 (MQ=255)
                                                                                                                tCTATTACAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTc                    >  1:129680/1‑141 (MQ=255)
                                                                                                                 cTATTACTACTGTTTCTGAATTGGACGGTTTTAACAGTAATACAAATTGTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTCCCGTTCACTCGTTTCCAAGATTACACAACCACCACAAATACACTTcc                   <  2:119072/141‑1 (MQ=255)
                                                                                                                       cAACTGTTTCTGAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTACTCCTACACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAg             <  1:163924/141‑1 (MQ=255)
                                                                                                                                  gAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATca  <  2:253921/141‑1 (MQ=255)
                                                                                                                                  gAATTGGACGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATca  <  2:93873/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
TGAATCATATTGTTCTAATCTTTTTGTTCGATATTTGAGTATGAATTTTAAAGATTATTTTACAAGTATTAAACTTGTTAACGCCATCAATTTATTACTTTCTACAAAACATTCTATTACAACTGTTTCTGAATTGGCCGGTTTTAACAGTCATACAAATTTTGCAAATCAATTTAAGAATTACTTGCATTTTAGTCCTAAACAGTTCCGTTCACTCGTTTCCAAGATTACTGAACCACCACAAATACACTTCCAACAAGATAACGTATCA  >  CP000730/2502004‑2502274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑