Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	707,879	A→T	L11I (TTA→ATA)	mntB ←	ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	707,879	0	A	T	100.0%	61.3 / NA	20	L11I (TTA→ATA)	mntB	ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/7);  total (13/7)

CCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATG  >  CP000730/707743‑708014                                                                                                                                         |                                                                                                                                        ccGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACCTGCTTATTACCTATAAAc                                                                                                                                     >  1:196837/1‑141 (MQ=255)          ttAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTaaa                                                                                                                            >  2:300717/1‑141 (MQ=255)             aaTTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCt                                                                                                                         <  2:253191/141‑1 (MQ=255)              aTTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCtt                                                                                                                        <  2:113692/141‑1 (MQ=255)                  aCCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGt                                                                                                                    >  1:225285/1‑141 (MQ=255)                       ttAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTa                                                                                                               >  2:237304/1‑141 (MQ=255)                                                  cAGCACCATTGGGGCCCATGATACCAATTCTTTCGCCGCCAACTGGTGTCGGTAAGGAAACGTTTTTAAGTACATGCTTACTGCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAACTaaaa                                                                                    >  2:343903/1‑141 (MQ=255)                                                    gCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGt                                                                                  >  1:247632/1‑141 (MQ=255)                                                            cGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTa                                                                          >  1:45758/1‑141 (MQ=255)                                                                   aTGATACCAATTATTTCGCCGCGTACTGGGAATGAATAGGAATTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaat                                                                   >  2:294240/1‑141 (MQ=255)                                                                                    gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                  >  2:249851/1‑141 (MQ=255)                                                                                                         ggAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                             <  2:233652/141‑1 (MQ=255)                                                                                                          gAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                            <  2:21355/141‑1 (MQ=255)                                                                                                              tGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                        >  1:124609/1‑141 (MQ=255)                                                                                                                tttttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTc                      >  2:120770/1‑141 (MQ=255)                                                                                                                  tttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATTCCAAGTAAATTCGt                    >  2:141973/1‑141 (MQ=255)                                                                                                                           aTGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataata           >  2:246211/1‑141 (MQ=255)                                                                                                                            tGCTTATGACCTATAAACAGATTTAAATCTTGTGTTTCTAACAAACGTTTATAGCTCCTAATTAAAAGTTTAGGCTAACCTAATGAAGTGTATAATAAACTGAGAATATTTATCATGGCAAGTAAAGTCGTGatataatat          <  2:50643/141‑1 (MQ=255)                                                                                                                                  ttaCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa    <  1:213348/141‑1 (MQ=255)                                                                                                                                    aCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg  <  1:213717/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATG  >  CP000730/707743‑708014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑