Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,015 C→T 25.9% R179R (AGG→AGA)  tpn ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0150CT25.9% 981.6 / inf 615R179R (AGG→AGA) tpnIS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (243/207);  new base T (86/73);  total (332/283)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 627 total aligned reads displayed.

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGAT  >  CP000730/35875‑36154
                                                                                                                                            |                                                                                                                                            
gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                                              >  2:437817/3‑141 (MQ=17)
gtttAACTTGCTAGAATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                                              >  1:358480/3‑141 (MQ=12)
  ttAACTTGCTAGCAGTATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                                            <  2:21902/141‑1 (MQ=12)
   tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                                           >  1:447678/1‑141 (MQ=17)
   tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                                           >  2:619131/1‑141 (MQ=17)
   tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                                           >  1:591403/1‑141 (MQ=17)
   tAACATGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                                           >  1:471522/1‑141 (MQ=17)
    aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                                                                          <  1:530244/141‑1 (MQ=32)
     aCTTGCTAGCATGATGCTAATTTCGTGGCAGGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGt                                                                                                                                         <  1:128641/141‑1 (MQ=11)
       ttGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                                                                                                                       <  1:513025/141‑1 (MQ=11)
       ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                                       <  2:73179/141‑1 (MQ=32)
          cTAGCATGCTGCTAATTTCGTGGCATGGCGAAATTCCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                    <  2:505002/141‑1 (MQ=12)
          cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCGATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                    <  1:427960/141‑1 (MQ=17)
           tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                                                                                                   <  1:261285/141‑1 (MQ=11)
           tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                                   >  2:407866/1‑141 (MQ=17)
           tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                                   <  2:539585/141‑1 (MQ=17)
            aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                                                                  >  2:545517/1‑141 (MQ=17)
             gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                                                                 <  1:252161/141‑1 (MQ=17)
              cATGATGCTAATTTCGTGGCATGGGAAAAATCCGTAGGGCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAc                                                                                                                                >  1:633450/1‑141 (MQ=11)
              cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTTTCTTTCTTAc                                                                                                                                >  2:516434/1‑141 (MQ=17)
               atgatgCTAATTTAGTGGCATGGCGAAAATCCGTAGTTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                                                                                               <  1:386922/141‑1 (MQ=16)
                 gatgCTAATTTCGTGGCATTGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                                             <  2:142821/141‑1 (MQ=255)
                  atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                                                                                                                            >  2:250522/1‑141 (MQ=21)
                    gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGATCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                                                                          >  1:351976/1‑141 (MQ=25)
                     cTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                                                                                                         >  2:459833/1‑141 (MQ=255)
                      tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAatat                                                                                                                        >  1:210343/1‑141 (MQ=255)
                          ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                                                                                                    >  2:11753/1‑141 (MQ=11)
                           tCGTGGCATGGGGAAAATCCGTAGAGCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACgg                                                                                                                   <  2:31229/141‑1 (MQ=11)
                            cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACAGt                                                                                                                  >  2:593156/1‑141 (MQ=11)
                              tGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAGTACATTCAATACCTTTTAAAGTAGTATTTGCCGTATTGATACTTTGATATTTTGTCTTTCTTTCTGTAAGATGACGATgg                                                                                                                >  1:175686/1‑140 (MQ=11)
                                   tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                                           <  2:373322/141‑1 (MQ=38)
                                   tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                                           <  2:401665/141‑1 (MQ=255)
                                      cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTTTTACTTTAATATGACGGTGATCTTA‑CTc                                                                                                       >  1:401615/1‑141 (MQ=255)
                                       gAAAATCCGTAGATCCCAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCa                                                                                                      <  1:50338/141‑1 (MQ=14)
                                         aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAAt                                                                                                    >  2:565373/1‑141 (MQ=37)
                                             ccGTAGATCTGAAGAGACCTGCGGTTCTTTGTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAgg                                                                                                >  1:661282/1‑141 (MQ=255)
                                              cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAACTTG‑CTCAATGCGGt                                                                                               >  2:380495/1‑141 (MQ=255)
                                               gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGtt                                                                                              <  1:234322/141‑1 (MQ=255)
                                                 aGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTAt                                                                                            <  1:497300/141‑1 (MQ=255)
                                                     cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAg                                                                                        <  2:259566/141‑1 (MQ=38)
                                                      tGAAGTGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGa                                                                                       <  2:101323/141‑1 (MQ=255)
                                                      tGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGa                                                                                       <  1:116087/141‑1 (MQ=255)
                                                          gagaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTG‑CTCAATGAGGTTATTCAGATAtt                                                                                   <  2:207203/141‑1 (MQ=255)
                                                                 tctgTTTTTTTTATATATAGCGTAAACACCCTTAATTCCTTTTAAATTATTCTTTGCTGTATTCATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGACCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGt                                                                            <  2:363872/138‑1 (MQ=11)
                                                                      tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                       >  1:268784/1‑141 (MQ=38)
                                                                        tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTTATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                                                                     <  2:94197/141‑2 (MQ=255)
                                                                         ttttATATAGAGCGTAAATACACTCAATACCTTTTATAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAGTggc                                                                    <  2:12890/141‑3 (MQ=255)
                                                                         ttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGTTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATggc                                                                    >  1:58133/1‑139 (MQ=255)
                                                                           ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGTTTCGTCAGATATTTCGATGTACAATGACAg                                                                  >  2:467979/1‑141 (MQ=255)
                                                                            tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                 <  2:179358/141‑1 (MQ=255)
                                                                              tataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATCTACAATGGCAGTCa                                                               <  1:365154/141‑1 (MQ=255)
                                                                               ataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                                              <  1:243153/141‑1 (MQ=255)
                                                                                taGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAACGACAGTCAgg                                                             <  1:610292/141‑1 (MQ=255)
                                                                                taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                                             <  1:331689/141‑1 (MQ=255)
                                                                                   agCGTAAATACATTCAATTCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                                          <  2:85571/141‑1 (MQ=255)
                                                                                   agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                                          <  2:388806/141‑1 (MQ=255)
                                                                                     cGTAAATACACTCAATACCTTTTAAAGTATTCTTGGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaa                                                        >  2:558190/1‑141 (MQ=255)
                                                                                      gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATGTCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGGTATTCAGATATTTGGATGTACAATGACAGTAGGgtttaag                                                       >  2:239161/1‑141 (MQ=14)
                                                                                        aaaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                     <  1:101004/141‑1 (MQ=255)
                                                                                         aaTACATTCAATACCTTTTAAAGTATTCTTTGGCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATCACGGTGCTCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAAGTGCAGTCAGgtttaagttt                                                    >  1:90592/1‑141 (MQ=11)
                                                                                          aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                   >  1:226304/1‑141 (MQ=255)
                                                                                          aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAGCTTG‑CTCAATGAGGTTATTCCGATATTTCGATGTACAATGGCGGTCAGgtttaagttta                                                   >  2:570526/1‑141 (MQ=14)
                                                                                           tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                  >  1:599896/1‑141 (MQ=255)
                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                  >  1:276420/1‑141 (MQ=255)
                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                  >  1:311966/1‑141 (MQ=255)
                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTC‑CTCAATGAGGTTATTCAGATATTTCGATCTACAATGCCAGAAAGgttcaactttaa                                                  >  1:136305/1‑141 (MQ=14)
                                                                                            acactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                 >  2:660218/5‑141 (MQ=255)
                                                                                             cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                >  2:601273/4‑141 (MQ=255)
                                                                                             cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTAATCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                >  2:342348/4‑141 (MQ=255)
                                                                                               ttCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGc                                              >  2:647560/1‑141 (MQ=255)
                                                                                                tCAATACCTTTTAAAGTATTCTTTGCTGTAGTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGGTATTCCGATATTTCGATGCACCACCGCAGTCAGGTTTAAGTTTAAAAGcg                                             <  2:491088/141‑2 (MQ=14)
                                                                                                     aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                        <  1:295852/141‑1 (MQ=255)
                                                                                                      ccTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTGAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                       >  1:322074/1‑141 (MQ=255)
                                                                                                       cTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCCTCAGCTA‑AAGAAGCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                      >  1:485854/1‑141 (MQ=14)
                                                                                                       cTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGTGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAATATTAAAAGATTTAAttt                                      >  1:200126/1‑140 (MQ=14)
                                                                                                              aGTATTCTTTGCTGTATTGATACTTTGATACCGTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaac                               <  2:158664/141‑3 (MQ=255)
                                                                                                                 aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt                            >  1:559477/1‑141 (MQ=255)
                                                                                                                  ttCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt                           <  1:544675/141‑1 (MQ=255)
                                                                                                                   tatttaacgggcggATACGTTGATCTCCTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg                          <  1:651667/128‑1 (MQ=14)
                                                                                                                    cTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc                         >  1:547746/1‑141 (MQ=255)
                                                                                                                      ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACCTTAACCATTGCTa                       >  2:480781/1‑141 (MQ=255)
                                                                                                                      ttGCTGTATGGATACGTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CGCAATGAGGTTATTCAGTTATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTATAAGCTTTAATTACTTTAACCATTGCTa                       <  2:347210/141‑1 (MQ=255)
                                                                                                                        tcTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc                     <  1:190120/140‑1 (MQ=255)
                                                                                                                           gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTG‑CTCAATGCGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTCCCTTc                  >  2:297314/1‑141 (MQ=14)
                                                                                                                           gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAAAGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTc                  >  1:411092/1‑141 (MQ=255)
                                                                                                                             tttGATACTTTGATACCTTGTCTTTCTTATTGTGATATGAAGGTGATGTTT‑CGCAATGAGGTTATTCAGCTATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGTTACCTTCGt                <  1:151499/140‑1 (MQ=11)
                                                                                                                             aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                <  2:634991/141‑1 (MQ=255)
                                                                                                                              ttGATACTTTGATAGCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGtt               >  2:583615/1‑141 (MQ=255)
                                                                                                                              ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGtt               >  2:197708/1‑141 (MQ=255)
                                                                                                                               tGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTT‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTg              >  1:157816/1‑141 (MQ=255)
                                                                                                                               tGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CACAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCtttt              >  1:443461/1‑140 (MQ=255)
                                                                                                                                 aTACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGaa            >  1:286922/1‑141 (MQ=255)
                                                                                                                                  tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg           <  1:369663/141‑1 (MQ=255)
                                                                                                                                  tACTTTGATACCTTCTCTTGCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg           >  1:435626/1‑141 (MQ=255)
                                                                                                                                  tACTTTGATACCCTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg           <  2:284778/141‑1 (MQ=255)
                                                                                                                                   aCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAgg          >  1:460308/1‑141 (MQ=40)
                                                                                                                                      ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGc       <  1:571357/141‑1 (MQ=38)
                                                                                                                                        gATACCTTGTCTTTCTTTCTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCt     <  1:80033/141‑1 (MQ=37)
                                                                                                                                         atatCTTGTCTGTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTg    <  1:341706/141‑1 (MQ=255)
                                                                                                                                          tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGa   >  2:63181/1‑141 (MQ=38)
                                                                                                                                            |                                                                                                                                            
GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTG‑CTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGAT  >  CP000730/35875‑36154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑