BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	85.1%	L58L (TTG→TTA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	85.1%	797.9 / 148.5	496	L58L (TTG→TTA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (32/41); new base T (204/210); total (243/253)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 497 total aligned reads displayed.

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAC  >  CP000730/36238‑36515

 gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACa                                                                                                                                          >  2:404105/1‑141 (MQ=255)

  cAACCAAATTGCTAATGTATGTCCCTCGGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                                                                                         <  2:311242/141‑1 (MQ=11)

      cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGGTTTTTTATGCTTTTTCTTCAAAATTTGATAGAAAAtt                                                                                                                                     >  1:484168/1‑141 (MQ=14)

      cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                                                                                     >  1:380302/1‑141 (MQ=255)

        aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACTTCTCATTAATACGCCATTTGTACTACGTTTTTTTGTGCTTTTTTTTCCGAATTTGATATAAAtttcg                                                                                                                                   >  1:513995/1‑139 (MQ=11)

        aaTATCTAATGTATGTCACTCTGTATCAAAGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAGAAAATTgg                                                                                                                                   >  2:217269/1‑141 (MQ=14)

         atatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTGTTTCTACCAAATTTGATATAAAATTggg                                                                                                                                  >  1:493128/1‑141 (MQ=11)

            tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATCATTTTTTTTCCAAATTTTCTATAAAATTtggcaa                                                                                                                               >  1:316804/1‑138 (MQ=11)

            tCTAATGTATGTCCCTCTGCATCAATGGCACGATATAACTAGCTCCATTTTCCTTTTCTTTTGATGTACGTCTCATCAATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCa                                                                                                                               <  2:140016/141‑1 (MQ=255)

             ttAACGTATTTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                              <  1:275589/140‑1 (MQ=11)

              tAATGTATTTCCTTTTGCATCAATTTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCata                                                                                                                             <  1:63181/141‑1 (MQ=14)

               aaTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCctat                                                                                                                            <  2:662501/141‑1 (MQ=255)

                atgtatgtCACTCTGGATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                           <  1:583615/141‑1 (MQ=255)

                    aagtaccTCTTCATCAATGGCACGATATAAATAGCTCCATATTCCTTTTATTTTGATGTACGACTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTAGTTTAAAATTGGGGAATcttcttt                                                                                                                       >  2:120563/6‑140 (MQ=11)

                      gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAAATTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGAGCATATTCTTGaa                                                                                                                     >  1:174980/1‑141 (MQ=11)

                       tCCCTCTGTATCAATGGCACGATATAACTAGCTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                    <  1:43354/141‑1 (MQ=11)

                           tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa                                                                                                                >  1:37569/1‑141 (MQ=255)

                              gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                             <  1:340769/141‑1 (MQ=40)

                              gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                             >  2:417144/1‑141 (MQ=40)

                               taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                            >  2:561582/2‑141 (MQ=255)

                               taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                            >  1:536063/2‑141 (MQ=21)

                                   aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACggaacac                                                                                                        >  2:42578/1‑136 (MQ=255)

                                     tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                      <  1:186729/141‑1 (MQ=40)

                                      ggCACGATATTAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                     <  1:241387/141‑1 (MQ=255)

                                      ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                     >  1:560486/1‑125 (MQ=25)

                                         aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGAGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                  >  1:447601/1‑141 (MQ=255)

                                          cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                 >  2:639338/1‑141 (MQ=40)

                                          cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGAATATTCTTGAACCCACCGGTAGATCg                                                                                                      >  1:467876/1‑136 (MQ=11)

                                           gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGGat                                                                                                >  1:614042/1‑141 (MQ=255)

                                           gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                                <  1:531579/141‑1 (MQ=255)

                                           gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCGAAATTTGATATAAAATTGGGGCATATTCTTGAACCAAACGGTAGAGCGTTGAat                                                                                                >  2:547354/1‑141 (MQ=11)

                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                               >  1:89131/1‑141 (MQ=25)

                                              ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                                                                             <  1:167793/141‑1 (MQ=255)

                                               taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAATATAACATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                                                                            >  1:290378/1‑141 (MQ=11)

                                                aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                           >  1:309386/1‑141 (MQ=21)

                                                  aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                                                                                         <  2:527939/141‑1 (MQ=18)

                                                  aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                                                                                         >  1:214800/1‑141 (MQ=21)

                                                  aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGAAGACCGTTGAATGATGAAc                                                                                         >  2:335847/1‑141 (MQ=18)

                                                     gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAATGCTGCACGtt                                                                                      >  2:233220/1‑141 (MQ=11)

                                                         cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                  >  1:438965/1‑141 (MQ=255)

                                                          aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                 <  1:409785/141‑1 (MQ=255)

                                                           ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                <  1:95822/141‑1 (MQ=21)

                                                           ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATTCTTGAACCCAACGGCAGCTCGTTGAATGATCAACGTTTAcacc                                                                                >  2:258180/1‑141 (MQ=11)

                                                            tttCCTTTTATTTTGATGTACGTCTCATCACTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCTGACGGTAGACCGTTGAATGATGACCGTTTAcacca                                                                               >  2:626121/1‑141 (MQ=11)

                                                             ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                              >  1:109458/1‑141 (MQ=25)

                                                              tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                             >  1:137032/1‑141 (MQ=255)

                                                               ccatttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                            >  2:113826/4‑141 (MQ=255)

                                                               ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                            >  1:100640/1‑141 (MQ=21)

                                                               ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                            <  1:267337/141‑1 (MQ=39)

                                                               ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGAAGATCGTTGAATAATTAACGTTTACACCACGt                                                                            >  2:376082/1‑141 (MQ=11)

                                                                cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGACAGTTTACACAACGtt                                                                           >  1:5550/1‑141 (MQ=11)

                                                                  tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGACGCATATTCTTGAACCCAATGGTAGATCGTTGCATGATGAACGTTTAACCCACGtcct                                                                         >  2:499143/1‑138 (MQ=11)

                                                                     tttttGATGTACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATTATGAATGTTTCCACCACGTTCCCtt                                                                      <  1:366743/140‑1 (MQ=11)

                                                                     agtttGATATACGTCTCATCTATACGCCATTTGTAATGAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCATCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                      <  2:532816/139‑1 (MQ=11)

                                                                       tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTCAATGATGAACGTTTACACCACGTTCTCTTaa                                                                    <  2:650681/141‑1 (MQ=11)

                                                                          gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                 >  2:28420/5‑141 (MQ=255)

                                                                          gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                 >  2:516674/5‑141 (MQ=255)

                                                                          gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                 >  1:330124/1‑141 (MQ=21)

                                                                           atatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCGCTTAATAtt                                                                >  1:127592/4‑141 (MQ=255)

                                                                            tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTACCAGTTGTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                               <  1:455442/139‑1 (MQ=11)

                                                                              tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                             >  2:274229/1‑141 (MQ=25)

                                                                               aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                            <  1:134631/141‑1 (MQ=255)

                                                                                cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                           <  1:592702/141‑1 (MQ=255)

                                                                                cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                           <  2:130167/141‑1 (MQ=255)

                                                                                 gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                          >  1:399203/1‑141 (MQ=255)

                                                                                    tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatata                                                       <  1:500269/141‑1 (MQ=25)

                                                                                    tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATTGGGGCATATTCTTGAACCCAACGTTAGAGCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                       <  1:524206/141‑1 (MQ=255)

                                                                                     catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                      >  2:600473/1‑141 (MQ=255)

                                                                                     catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatatat                                                      <  1:646468/141‑1 (MQ=18)

                                                                                      atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTAAATATTTCGTATATGTc                                                     >  1:234358/1‑141 (MQ=11)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAATATTTCAGATATATcc                                                    >  2:168853/1‑140 (MQ=255)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                    <  1:546038/141‑1 (MQ=21)

                                                                                         aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                  >  1:576765/1‑141 (MQ=255)

                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                 <  1:15407/141‑1 (MQ=39)

                                                                                          aTACGCCATGTGTAATAAGCTNTNTTANGCTTTTTCGTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                 <  1:351250/141‑1 (MQ=255)

                                                                                           tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                <  1:194336/141‑1 (MQ=255)

                                                                                           tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTTAACCCAACCGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                <  2:570777/141‑1 (MQ=255)

                                                                                            aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                               <  2:384291/141‑1 (MQ=255)

                                                                                             cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                              <  2:73041/141‑2 (MQ=11)

                                                                                              cctctttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGTCTTATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCCGATATATCACGATagc                                             <  2:607401/137‑3 (MQ=11)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                            <  2:295109/141‑1 (MQ=21)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTAAGATATATCACGATAGCt                                            >  2:480658/1‑141 (MQ=11)

                                                                                                cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                           <  2:192524/141‑1 (MQ=21)

                                                                                                 aTTTGTAATAAGCTTTTTTATGCTTCTACTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                          <  2:343036/141‑1 (MQ=11)

                                                                                                  tttGTTATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                         <  1:8243/141‑1 (MQ=11)

                                                                                                  tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                         >  1:285297/1‑141 (MQ=21)

                                                                                                   ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                        >  1:202949/1‑141 (MQ=255)

                                                                                                   ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCATCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                        <  2:238182/141‑1 (MQ=255)

                                                                                                    ttgaataaGCTTTTTTATGCGTTGTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAGCCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                       <  1:120111/138‑1 (MQ=11)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                       <  1:29547/141‑1 (MQ=11)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                       >  2:158804/1‑141 (MQ=21)

                                                                                                     gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                      <  1:603602/141‑2 (MQ=11)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACAACACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                     <  2:356831/141‑3 (MQ=12)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCGTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTGTTAATATTTTAGCTATATCACGATTGCTCAAttca                                     >  2:558031/1‑138 (MQ=11)

                                                                                                              ttttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAg                             >  1:663701/1‑141 (MQ=35)

                                                                                                                  ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACCCAATGTATATCTTACAtaa                         >  2:436815/1‑140 (MQ=21)

                                                                                                                  ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTACCTTAATATTTCATGTATATCACGATAACTCAATGTATATCTTAGAtca                         >  2:464318/1‑139 (MQ=255)

                                                                                                                    aTGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                       >  1:424761/1‑141 (MQ=17)

                                                                                                                        tttttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCa                   <  2:450838/141‑1 (MQ=35)

                                                                                                                                    aTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTg       <  1:395460/141‑1 (MQ=35)

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAC  >  CP000730/36238‑36515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`