Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,876 C→T 44.6% intergenic (+107/+1) rlmH → / ← tpn hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8760CT44.6% 109.8 / 139.8 137intergenic (+107/+1)rlmH/tpnhypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (31/43);  new base T (31/30);  total (64/73)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 141 total aligned reads displayed.

AGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35736‑36016
                                                                                                                                            |                                                                                                                                            
aGCTATTCAGTATAAAGTTGTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAGATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                                              >  1:400804/1‑141 (MQ=255)
    aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGATTaa                                                                                                                                          >  2:610292/1‑141 (MQ=255)
      tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATATAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATATCAGTGTTCGCTTacgt                                                                                                                                        >  2:27577/1‑138 (MQ=255)
       cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTTTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCCGTGTTCGCTTAACtt                                                                                                                                       >  1:291867/1‑141 (MQ=255)
          tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                                                    <  1:166699/141‑1 (MQ=255)
            aaaaGTTTTGGGTGAAAAATGATTACATGAACATGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAg                                                                                                                                  <  2:375255/140‑1 (MQ=255)
              aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAGTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                                                                                >  1:521302/1‑141 (MQ=255)
              aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATGGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                                                                                >  2:571357/1‑141 (MQ=255)
              aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTTCTAGCa                                                                                                                                >  2:200779/1‑141 (MQ=255)
              aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                                                                                >  2:529422/1‑141 (MQ=255)
               aGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGCAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCCCTAATTTCTAGTGTCAGTGTTCCATTaagcggcgagcat                                                                                                                               >  2:69154/1‑130 (MQ=25)
                gTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCAAGTCGGTGTTCGCTTAACTTGATAGAatg                                                                                                                              >  1:13971/1‑141 (MQ=255)
                      ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                                                                                        >  2:534511/1‑141 (MQ=255)
                       gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                                                                                       >  2:122052/1‑141 (MQ=255)
                             aaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                                                                                 <  2:341772/141‑1 (MQ=255)
                             aaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                                                                                 <  1:149445/141‑1 (MQ=255)
                                    aCTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATgg                                                                                                          <  1:147728/141‑1 (MQ=255)
                                     cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                                                                         <  1:26438/141‑1 (MQ=255)
                                          aCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaaa                                                                                                    <  1:541593/141‑1 (MQ=255)
                                             agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                                                 >  1:16745/1‑141 (MQ=255)
                                               agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGGATGGCGAAAATCCGt                                                                                               <  1:496999/141‑1 (MQ=255)
                                               agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                                               >  2:198377/1‑141 (MQ=255)
                                                    aaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCTTGGCGAAAATCCGTAGATc                                                                                          <  2:308879/141‑1 (MQ=255)
                                                        aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGGTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                    <  1:349442/129‑1 (MQ=11)
                                                        aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                    <  1:89682/129‑1 (MQ=11)
                                                          aatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagg                                                                                    >  2:560951/1‑140 (MQ=255)
                                                           atTGTTATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTCATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                                                   <  2:483979/141‑1 (MQ=255)
                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATTTCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:450627/132‑1 (MQ=11)
                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 >  1:523371/10‑141 (MQ=11)
                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:92621/132‑1 (MQ=11)
                                                           agagtattttaataaAAATGATGGGTCTGGTGCAAAGTAAAAAATATAGCTAATCACTATTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:69897/132‑1 (MQ=11)
                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:535159/141‑1 (MQ=255)
                                                             tgtgATCTAATAAAAATAGAAGGTTATGTTGCAAAGTAAAAAAGTATAGCTAACCCCTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:253781/141‑1 (MQ=255)
                                                             ggggATCTAATAAAAATAGAAGGTTATGGTGAAAAGTAAAAAAAGATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTGGCTAGCATGATTCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:395592/138‑1 (MQ=14)
                                                             agtattttcataaAAATGATGGTTCTGTTGTAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  1:459642/132‑1 (MQ=11)
                                                             aggattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTGGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  2:275324/134‑1 (MQ=11)
                                                              gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  2:241647/141‑1 (MQ=255)
                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTTGTGTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  1:654593/135‑1 (MQ=11)
                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATCCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  2:225966/135‑1 (MQ=11)
                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAAATTGCTAGCTTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  2:305666/135‑1 (MQ=11)
                                                               tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  2:209768/141‑1 (MQ=255)
                                                               tattttaataaACATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTAGTGGCATGGCGAATATCTGTAGATCTGGAGAGAACTgg                                                                             >  1:166184/6‑140 (MQ=11)
                                                               tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             <  1:6332/136‑1 (MQ=11)
                                                                attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  2:187903/137‑1 (MQ=11)
                                                                attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  2:275314/137‑1 (MQ=11)
                                                                attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  2:437578/137‑1 (MQ=11)
                                                                 ttttaataaAAATGATTGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTGCgg                                                                           >  1:332441/4‑141 (MQ=11)
                                                                 aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             >  1:618958/1‑141 (MQ=255)
                                                                 aTCTAATAAAAAGATGAGGTTCTGTTGGAAAGTAAAAAAATATAGCTAACCACTCATTTATCAAGTCAGTGGTCGCTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             <  2:237962/141‑1 (MQ=11)
                                                                  tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGGGACCTGCGGt                                                                          >  1:12890/3‑141 (MQ=11)
                                                                  tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  1:260334/141‑1 (MQ=255)
                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                         >  1:201000/2‑141 (MQ=11)
                                                                    taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGttt                                                                        >  2:295852/1‑140 (MQ=11)
                                                                      ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                                                      >  1:439334/1‑141 (MQ=11)
                                                                       taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     >  1:620447/1‑141 (MQ=11)
                                                                          aaaTAGACGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCCTGTAAGTGTTCGCTTAACTTGCTAGCATAATGCTAATTACGTGGCATGGCGAAAATCCGTAGAACTGAAGAGACCTGCGGTTCtttt                                                                    >  1:421487/1‑141 (MQ=11)
                                                                             aaGAATGTTCTGTTGCAAAGTAAAAAAATCTAGCTAACCACTAATTTATCATGTCAGTGTCCGCTTAACTTTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                                                 <  2:58412/140‑1 (MQ=255)
                                                                                aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                                              >  2:547626/1‑141 (MQ=11)
                                                                                aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCGGCGGTTCTTTTtatata                                                              >  1:86045/1‑141 (MQ=11)
                                                                                   tttttGTTGCAAAGTAAAAAATATCGCTAACCACCAATATATCATGTCAGTGGTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGc                                                          <  1:282612/137‑1 (MQ=1)
                                                                                    ttttGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCg                                                         <  2:645702/138‑1 (MQ=1)
                                                                                                                aatcACTAATATATCATGTCAGTTTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGATGTTTTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa                              <  1:352877/138‑1 (MQ=1)
                                                                                                                     ctcaTTTATCGTGACAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCtt                         <  1:393780/138‑1 (MQ=0)
                                                                                                                           tttcctGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGccgt                   <  2:232854/136‑4 (MQ=1)
                                                                                                                                   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAGAGTATTCTTTGCCGTATTGATAc           >  2:563653/1‑141 (MQ=11)
                                                                                                                                   cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  2:618958/141‑1 (MQ=11)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGGATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTGTTAAAGTATTCTTTGCCGTATTGATACt          >  1:346126/1‑141 (MQ=11)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTCTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGACGTATTGATACt          >  2:384544/1‑141 (MQ=11)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  1:457848/1‑141 (MQ=14)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCAAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATGCCTTTTAAAGTATTCTTGGCCGTATTGATACt          >  2:123859/1‑141 (MQ=11)
                                                                                                                                    aGTGTTCGTTCAACCTGCTAGCATGATGCTAATTCCCTAGCCAAGCGAAAACAACTCGTTCTGTAGAGATCTGCGGTCCTTTTTATATAGAGCGTAACTACACTCAATACCGTTTAAAGTATTCTTTGCTGTAATGATACt          >  2:110901/1‑141 (MQ=2)
                                                                                                                                    aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTACAGTATTCTTTTCTGTATTGATACt          >  2:431936/1‑141 (MQ=14)
                                                                                                                                    aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  2:444909/1‑141 (MQ=31)
                                                                                                                                    aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGGATTCTTTGCTGTATTGATACt          >  1:286614/1‑141 (MQ=14)
                                                                                                                                     gttttCGTTTAACTTGCTAGCATGATGCTTATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         <  2:137669/138‑1 (MQ=255)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGCAAGTCCTGAGGTCTGAAGAAGCCTACGGTTCTTCTTATATACAGCGTAAATACATTCAATACATTTTAAATTATTCTTTGCCGTATTGATACtt         >  2:109178/1‑141 (MQ=11)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt         >  2:48420/1‑141 (MQ=255)
                                                                                                                                     gtgtTCGTTTAAATTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt         <  2:209692/141‑1 (MQ=11)
                                                                                                                                     gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGGAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:211052/1‑141 (MQ=17)
                                                                                                                                     gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:505803/1‑141 (MQ=17)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt        >  2:286649/1‑141 (MQ=255)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt        >  2:427240/1‑141 (MQ=11)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCCTTTAAAGTATTCTTTGCTGTATTGATACttt        >  2:201574/1‑141 (MQ=12)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGGAAATGCACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt        >  2:331683/1‑141 (MQ=255)
                                                                                                                                      tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt        <  1:362133/141‑1 (MQ=17)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTCGATCTGAAGAGACCTACGGTTCTTTTTATATAAAGCGTAAATCCATTCAATACCTTTTAAAGTATTCGTTGCCGTTTTGATGCtttt       >  2:462654/1‑140 (MQ=11)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg       >  1:536140/1‑141 (MQ=17)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGAATTGATACATTg       >  2:265213/1‑141 (MQ=9)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATACGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCATTTAAAGTATACTTTGCTGTATTGATACTTTg       >  2:301752/1‑141 (MQ=11)
                                                                                                                                       gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACTTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg       <  1:499789/141‑1 (MQ=17)
                                                                                                                                       gctCGCTTACCTTGGTAGCAGTATGGTAATTTCGTCGCATGGCGAAAACCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCCGAAACACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg       <  2:21708/139‑1 (MQ=11)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCCTTCTCAATACGGCGTAAAAACACTCAATACCTTTTAAAGTATTCTTTGCTGTATGGATACTTTGa      <  2:641184/141‑1 (MQ=11)
                                                                                                                                         tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  1:143416/141‑1 (MQ=32)
                                                                                                                                         tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATGCTTTGAt     >  1:29749/1‑141 (MQ=17)
                                                                                                                                         tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  2:180417/141‑1 (MQ=17)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  2:263966/1‑141 (MQ=32)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  2:624222/1‑141 (MQ=17)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  1:444887/141‑1 (MQ=17)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATACATAGCGTAATTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  1:421051/141‑1 (MQ=255)
                                                                                                                                            cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  1:325223/141‑1 (MQ=17)
                                                                                                                                            |                                                                                                                                            
AGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35736‑36016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: