Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,799 C→A 28.5% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,7990CA28.5% 132.0 / 50.3 84intergenic (‑882/‑286)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/31);  new base A (11/13);  total (39/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGC  >  CP000730/1929661‑1929939
                                                                                                                                          |                                                                                                                                                 
tCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCga                                                                                                                                                 <  2:662911/141‑1 (MQ=255)
 cATAATAAAATCGCCTAATTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTACGTGAAGACGATTCAACCTATCTGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATGTTTGCCgag                                                                                                                                                >  2:2023/1‑140 (MQ=25)
            cGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                     <  1:363320/141‑1 (MQ=255)
            cGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                     >  2:229980/1‑141 (MQ=255)
              ccTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagtag                                                                                                                                   >  2:438189/1‑141 (MQ=255)
              ccTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagtag                                                                                                                                   >  1:619904/1‑141 (MQ=255)
                   tttCTTAAATTAACAATATCAATTCTCAAAGAATTTGTCCAATTAAGTGTAGACTATTCAATCTATCAGCTAGAATCATCTAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGACg                                                                                                                              >  1:432165/1‑141 (MQ=38)
                      tttAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                           <  1:377162/140‑1 (MQ=255)
                      cTTAAATTAACACTATCTATGCTCCTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTCTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                           <  1:508562/141‑1 (MQ=255)
                      cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGGGCCGCCt                                                                                                                           >  2:425465/1‑141 (MQ=255)
                      cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                           <  1:410201/141‑1 (MQ=255)
                       ttAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTGGCCTa                                                                                                                          >  1:394030/1‑141 (MQ=255)
                              aaCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCa                                                                                                                   <  2:550975/141‑1 (MQ=255)
                                cAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAgg                                                                                                                 <  1:266783/141‑1 (MQ=255)
                                   tatCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGt                                                                                                              >  1:7212/1‑141 (MQ=255)
                                    atCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTc                                                                                                             >  1:198947/1‑141 (MQ=255)
                                     tCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAATGGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCa                                                                                                            <  1:91965/141‑1 (MQ=255)
                                     tCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCa                                                                                                            <  1:264123/141‑1 (MQ=255)
                                     tCTATTCTCAGAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCa                                                                                                            <  1:524843/141‑1 (MQ=255)
                                        aTTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAtt                                                                                                         >  2:51669/1‑141 (MQ=255)
                                                aGAATTTGTCCAATTAAGTGTAGCCGATTCAATCTCTCAGCTAGAATCTTATGACTGGTCAGAAGCAATTGACTGGTCTTGTAGATTTGCGGATGGTATAATAGTAGAGGCGCCTATCTCTCAGGCGTCAATTTAGACGCa                                                                                                 <  2:223840/141‑1 (MQ=255)
                                                aGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCa                                                                                                 <  2:120252/141‑1 (MQ=255)
                                                   atgtGTCCAATTAAGGGTAGAGGATTCAACCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                              <  2:302552/138‑1 (MQ=255)
                                                   aTTTGTCCAATTAAGTGTAGCCGGTTCCTTCAACTGCCTAGAGTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGGAGAGTCGGCTAGCTCTCTGGCGTCAATTTAGACGCagag                                                                                              <  2:302490/141‑1 (MQ=255)
                                                     ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                            >  2:52323/1‑141 (MQ=255)
                                                     ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTACGGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                            <  2:110751/141‑1 (MQ=255)
                                                     ttGGCCAATGAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTTCTTGTATATGCGCCGAGGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                            <  2:198903/141‑1 (MQ=255)
                                                       gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGCAGCAAGTGACTGTGCTTGTATATTTGCCGATGATAAGATAGTAGAGTCGCCTATCTCTCAGGCGACAATTTGGACGCAGAgaggg                                                                                          >  1:262162/1‑140 (MQ=255)
                                                          cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgt                                                                                       <  2:359265/141‑1 (MQ=255)
                                                          cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAGTTAGACGCGGAGGGGAGgt                                                                                       >  1:518861/1‑141 (MQ=255)
                                                               aaGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGtata                                                                                 <  2:334283/141‑1 (MQ=255)
                                                               aaGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGtata                                                                                 <  1:385609/141‑1 (MQ=255)
                                                                 gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAg                                                                              <  2:104716/141‑1 (MQ=255)
                                                                 gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGGTATAAg                                                                              >  1:33137/1‑141 (MQ=255)
                                                                 gtggAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAg                                                                              <  2:469274/141‑1 (MQ=255)
                                                                              aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAttt                                                              <  2:55256/141‑1 (MQ=255)
                                                                               aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGGGGAGGTGTATAAGGGGGTGCTTGtttt                                                             >  1:67697/1‑141 (MQ=255)
                                                                               aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAtttt                                                             >  1:617662/1‑141 (MQ=255)
                                                                                           gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCAcatcat                                                 >  2:589440/1‑141 (MQ=255)
                                                                                            aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGCTAAAAGATTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATa                                                <  1:625249/141‑1 (MQ=255)
                                                                                            aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATa                                                <  1:40282/141‑1 (MQ=255)
                                                                                            aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATa                                                <  1:40471/141‑1 (MQ=255)
                                                                                             aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAg                                               >  1:104477/1‑141 (MQ=255)
                                                                                             aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAg                                               >  2:651616/1‑141 (MQ=255)
                                                                                                  ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCAATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCa                                          >  1:456894/1‑141 (MQ=255)
                                                                                                     aCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCTGtc                                       >  2:546668/1‑141 (MQ=255)
                                                                                                           cAGAAGCAAGTGACTGTGCTTGTTCTTGAACCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                 >  1:661663/1‑141 (MQ=255)
                                                                                                           cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                 <  2:447099/141‑1 (MQ=255)
                                                                                                           cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                 >  2:655242/1‑141 (MQ=255)
                                                                                                            aGAAGCAAGTGACTGTGTTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTg                                <  1:501325/141‑1 (MQ=255)
                                                                                                            aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTGGTTCACATCATAGCACCAGGAATCAGTg                                >  1:255828/1‑141 (MQ=255)
                                                                                                             atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                              >  1:621917/15‑141 (MQ=255)
                                                                                                              tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                             >  1:443158/14‑141 (MQ=255)
                                                                                                                tgaagtcgatatccTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                           <  1:509801/128‑1 (MQ=255)
                                                                                                                ggaagtaggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                           <  1:82708/130‑1 (MQ=255)
                                                                                                                ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                           >  1:168329/12‑141 (MQ=255)
                                                                                                                ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTAATAAGTGGCtgt                           >  1:368623/12‑141 (MQ=255)
                                                                                                                gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACGTAATAGCAGCAGTCATCAGTGGCtg                            >  2:189474/1‑141 (MQ=255)
                                                                                                                gCAAGTGACTGTGCTTGTATATTTACCGATGATATAATAGTAGAGTCGCCTATCTCGCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                            >  2:490596/1‑141 (MQ=255)
                                                                                                                  aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                         <  1:442291/132‑1 (MQ=255)
                                                                                                                  aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                          >  1:291877/1‑141 (MQ=255)
                                                                                                                   agtagatattcTTCTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                        <  2:500018/131‑2 (MQ=255)
                                                                                                                   agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                        <  2:618990/133‑2 (MQ=255)
                                                                                                                   agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                        <  1:430107/133‑2 (MQ=255)
                                                                                                                    gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                       <  1:543834/134‑3 (MQ=255)
                                                                                                                    gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                       >  1:45839/8‑139 (MQ=255)
                                                                                                                    gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                       <  1:170476/134‑3 (MQ=255)
                                                                                                                    gcagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                       >  2:578301/8‑139 (MQ=255)
                                                                                                                      gACTGTGCTTGTATATTTGCCGATCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCCCATCATAGCACCAGTCAGCAGTGGCTGTGCCAt                      <  1:134979/141‑1 (MQ=255)
                                                                                                                      agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgct                     >  1:476726/6‑137 (MQ=255)
                                                                                                                        atatgagtGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                   <  1:71702/134‑7 (MQ=255)
                                                                                                                        atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                   <  1:603429/138‑7 (MQ=255)
                                                                                                                         tatgattGTATATTCACAGATGGTATAATAATAGAATCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTGGAGCATCATTGTCGGTCACAGCATAGCCCCAGTCATCAGTGGCCGTGCCGGTGCg                  >  2:41985/6‑141 (MQ=255)
                                                                                                                         tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCGGTGGCTGTGCTGTTGCg                  >  1:117086/3‑141 (MQ=255)
                                                                                                                          ttgCTTGTATAGTTGCCGATGATATAATAGTAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                  <  2:101255/140‑1 (MQ=255)
                                                                                                                          gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                  >  1:416956/1‑141 (MQ=255)
                                                                                                                          atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                 <  2:516675/140‑1 (MQ=255)
                                                                                                                           tgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                >  1:32426/1‑140 (MQ=255)
                                                                                                                             cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt              <  1:557506/141‑4 (MQ=255)
                                                                                                                              ttGTATATTCACAGATGGGATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGtatta             <  1:594485/141‑2 (MQ=255)
                                                                                                                                gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATAATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGtattata           >  2:374439/1‑138 (MQ=255)
                                                                                                                                 tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATTCTTATCTTCGTTCGCATCATAGCACCAGTCATCAGTGCCTGTGCCATTGCGttttttt           <  1:407313/141‑1 (MQ=255)
                                                                                                                                   aatTTGCCGATGATATAATAGGAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt         <  2:262162/140‑1 (MQ=255)
                                                                                                                                    atTTGCCGATGATATAATAGTAGAGTCGACTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt        <  1:463886/141‑1 (MQ=255)
                                                                                                                                          cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgtt  >  2:606001/1‑139 (MQ=255)
                                                                                                                                          cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc  <  2:40546/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
TCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGC  >  CP000730/1929661‑1929939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: