Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,848 C→A 58.3% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8480CA58.3% 42.7 / 164.5 134intergenic (‑931/‑237)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/29);  new base A (47/31);  total (74/60)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

Only 100 of 134 total aligned reads displayed.

AGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAA  >  CP000730/1929709‑1929987
                                                                                                                                           |                                                                                                                                                 
aGAATTTGTCCAATTAAGTGTAGCCGATTCAATCTCTCAGCTAGAATCTTATGACTGGTCAGAAGCAATTGACTGGTCTTGTAGATTTGCGGATGGTATAATAGTAGAGGCGCCTATCTCTCAGGCGTCAATTTAGACGCa                                                                                                                                                  <  2:223840/141‑1 (MQ=255)
   aTTTGTCCAATTAAGTGTAGCCGGTTCCTTCAACTGCCTAGAGTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGGAGAGTCGGCTAGCTCTCTGGCGTCAATTTAGACGCagag                                                                                                                                               <  2:302490/141‑1 (MQ=255)
     ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                                                             >  2:52323/1‑141 (MQ=255)
     ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTACGGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                                                             <  2:110751/141‑1 (MQ=255)
     ttGGCCAATGAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTTCTTGTATATGCGCCGAGGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                                                             <  2:198903/141‑1 (MQ=255)
       gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGCAGCAAGTGACTGTGCTTGTATATTTGCCGATGATAAGATAGTAGAGTCGCCTATCTCTCAGGCGACAATTTGGACGCAGAgaggg                                                                                                                                           >  1:262162/1‑140 (MQ=255)
          cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgt                                                                                                                                        <  2:359265/141‑1 (MQ=255)
          cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAGTTAGACGCGGAGGGGAGgt                                                                                                                                        >  1:518861/1‑141 (MQ=255)
               aaGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta‑ta                                                                                                                                  <  1:385609/141‑1 (MQ=255)
                 gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑g                                                                                                                               <  2:104716/141‑1 (MQ=255)
                 gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGGTA‑TAA‑g                                                                                                                               >  1:33137/1‑141 (MQ=255)
                               aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGGGGAGGTGTA‑TAA‑G‑G‑G‑GGTGC‑TTGtttt                                                                                                             >  1:67697/1‑141 (MQ=255)
                                           gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCAcatcat                                                                                                 >  2:589440/1‑141 (MQ=255)
                                            aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGCTAAAAGATTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATa                                                                                                <  1:625249/141‑1 (MQ=255)
                                            aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATa                                                                                                <  1:40471/141‑1 (MQ=255)
                                            aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATa                                                                                                <  1:40282/141‑1 (MQ=255)
                                             aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAg                                                                                               >  1:104477/1‑141 (MQ=255)
                                                  ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCAATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCa                                                                                          >  1:456894/1‑141 (MQ=255)
                                                     aCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCTGtc                                                                                       >  2:546668/1‑141 (MQ=255)
                                                           cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                                                                 >  2:655242/1‑141 (MQ=255)
                                                           cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                                                                 <  2:447099/141‑1 (MQ=255)
                                                            aGAAGCAAGTGACTGTGTTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTg                                                                                <  1:501325/141‑1 (MQ=255)
                                                            aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTGGTTCACATCATAGCACCAGGAATCAGTg                                                                                >  1:255828/1‑141 (MQ=255)
                                                             atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                              >  1:621917/15‑141 (MQ=255)
                                                                ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                           >  1:168329/12‑141 (MQ=255)
                                                                ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTAATAAGTGGCtgt                                                                           >  1:368623/12‑141 (MQ=255)
                                                                gCAAGTGACTGTGCTTGTATATTTACCGATGATATAATAGTAGAGTCGCCTATCTCGCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                            >  2:490596/1‑141 (MQ=255)
                                                                  aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                         <  1:442291/132‑1 (MQ=255)
                                                                   agtagatattcTTCTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                        <  2:500018/131‑2 (MQ=255)
                                                                   agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                        <  1:430107/133‑2 (MQ=255)
                                                                    gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                       <  1:543834/134‑3 (MQ=255)
                                                                    gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                       <  1:170476/134‑3 (MQ=255)
                                                                    gcagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                       >  2:578301/8‑139 (MQ=255)
                                                                      agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgct                                                                     >  1:476726/6‑137 (MQ=255)
                                                                        atatgagtGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                   <  1:71702/134‑7 (MQ=255)
                                                                        atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                   <  1:603429/138‑7 (MQ=255)
                                                                         tatgattGTATATTCACAGATGGTATAATAATAGAATCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑T‑GGAGCATCATTGTCGGTCACAGCATAGCCCCAGTCATCAGTGGCCGTGCCGGTGCg                                                                  >  2:41985/6‑141 (MQ=255)
                                                                         tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCGGTGGCTGTGCTGTTGCg                                                                  >  1:117086/3‑141 (MQ=255)
                                                                          ttgCTTGTATAGTTGCCGATGATATAATAGTAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                                                                  <  2:101255/140‑1 (MQ=255)
                                                                          gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                                                                  >  1:416956/1‑141 (MQ=255)
                                                                           tgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                                                                >  1:32426/1‑140 (MQ=255)
                                                                              ttGTATATTCACAGATGGGATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGtatta                                                             <  1:594485/141‑2 (MQ=255)
                                                                                gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATAATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGtattata                                                           >  2:374439/1‑138 (MQ=255)
                                                                                 tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtgttatac                                                          >  1:187644/11‑137 (MQ=255)
                                                                                 tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatat                                                          >  2:605751/11‑137 (MQ=255)
                                                                                 tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TGGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                          >  1:451585/11‑137 (MQ=255)
                                                                                 tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TAATTTTCGTTCACATCATAGCACCAGGCATCAGTGGCTGTGCTGTTGCGtattatac                                                          >  2:605744/11‑137 (MQ=255)
                                                                                 tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATTC‑TTATCTTCGTTCGCATCATAGCACCAGTCATCAGTGCCTGTGCCATTGCGttttttt                                                           <  1:407313/141‑1 (MQ=255)
                                                                                  atattcacagatggtATAATAATAGAGTCGCGTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTACACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                         >  1:491760/10‑136 (MQ=255)
                                                                                   tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACtt                                                        >  1:303874/9‑141 (MQ=255)
                                                                                   aatTTGCCGATGATATAATAGGAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                         <  2:262162/140‑1 (MQ=255)
                                                                                    tatcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                       >  2:337992/8‑141 (MQ=255)
                                                                                    attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                       >  2:121783/8‑141 (MQ=255)
                                                                                    atTTGCCGATGATATAATAGTAGAGTCGACTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                                                        <  1:463886/141‑1 (MQ=255)
                                                                                        acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGGTGCATTAC‑T‑T‑T‑GCTAG‑TCCTTGTCGCTCACATCAGAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                   >  2:640275/4‑141 (MQ=255)
                                                                                         cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                  >  1:9688/3‑141 (MQ=255)
                                                                                          cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgtt                                                  >  2:606001/1‑139 (MQ=255)
                                                                                             tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                              >  1:183012/4‑141 (MQ=255)
                                                                                              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATTATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                             <  2:20863/139‑1 (MQ=255)
                                                                                              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                             >  2:621136/3‑141 (MQ=255)
                                                                                              ggtacaatgttagAGTCGCCTATCTCCTAGGCGGCACTT‑‑GCCGAAGAGAGGAGGTGCATTCC‑T‑T‑T‑GCTAG‑TCATTTCCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                             <  2:112381/131‑1 (MQ=255)
                                                                                               gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGCTCACCTCAAAACACCTGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                            >  1:126114/2‑141 (MQ=255)
                                                                                               atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                             >  2:647888/1‑141 (MQ=255)
                                                                                                 ctaataaGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                          <  1:533308/140‑3 (MQ=255)
                                                                                                 ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                          >  1:145633/1‑139 (MQ=255)
                                                                                                  taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                         <  1:220128/141‑4 (MQ=255)
                                                                                                  taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑GCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATAATTATTGGCttggtaaac                                         >  1:91844/1‑133 (MQ=255)
                                                                                                    ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                       >  2:336579/5‑141 (MQ=255)
                                                                                                    ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTacctcgacc                                        >  2:429641/1‑133 (MQ=255)
                                                                                                     aaatagAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                      <  2:581824/138‑1 (MQ=255)
                                                                                                        tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                   >  1:293777/1‑139 (MQ=255)
                                                                                                        tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                   <  2:299261/141‑3 (MQ=255)
                                                                                                        tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                   >  1:2819/1‑139 (MQ=255)
                                                                                                          gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                  <  1:459516/141‑1 (MQ=255)
                                                                                                          gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                 >  2:282973/1‑137 (MQ=255)
                                                                                                           agTCCCCTATCTCTCAGGCGTCAATTTATACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                 <  1:65601/141‑1 (MQ=255)
                                                                                                            gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAACCGCaacaaata                               >  1:213076/1‑135 (MQ=255)
                                                                                                            gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                               >  2:430302/1‑135 (MQ=255)
                                                                                                             tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                              >  2:497279/1‑134 (MQ=255)
                                                                                                               gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                            >  2:154913/1‑132 (MQ=255)
                                                                                                               gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                            <  1:464365/141‑10 (MQ=255)
                                                                                                               gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGGTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                            <  1:456075/141‑10 (MQ=255)
                                                                                                                 cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCGGTCGTCATCGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                          >  1:154206/1‑130 (MQ=255)
                                                                                                                 cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGGGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                          <  2:45839/141‑12 (MQ=255)
                                                                                                                  tacccctcAGGCGTAAATTTAGACGCAGAGAGGAGGTGGA‑TAA‑G‑G‑T‑GAGGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAgg                          <  1:190978/136‑1 (MQ=255)
                                                                                                                  tATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGTGGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATTAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                         <  2:372869/141‑13 (MQ=255)
                                                                                                                  tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                         >  2:17659/1‑129 (MQ=255)
                                                                                                                  tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                         >  2:269331/1‑129 (MQ=255)
                                                                                                                  tATCTCTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTGCA‑TAACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCCCCAGTCATCAGGGGCTGTGCTGTTGCGTATTATACTTATTGGCTGAGTAAACGCaacaaataattaat                         <  1:603319/141‑13 (MQ=255)
                                                                                                                   aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAC‑TCATTTTCTTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                        <  1:349945/141‑14 (MQ=255)
                                                                                                                   aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaatccatactc                        >  2:328789/1‑128 (MQ=255)
                                                                                                                    tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGCCTAAGTAGCAGCCATACAACATAGGTg                        >  2:528254/1‑141 (MQ=255)
                                                                                                                    tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTTGTAAACGCaacaaataattaatag                       <  1:426032/141‑15 (MQ=255)
                                                                                                                    tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattcatag                       >  2:195512/1‑127 (MQ=255)
                                                                                                                       ctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACa                     >  2:389607/1‑141 (MQ=255)
                                                                                                                       cccacgCGTCAATTTATCCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCAGCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACa                     <  1:401522/136‑1 (MQ=255)
                                                                                                                         cAGGCGTCAATTTAGACGAAGAGAGGAGGTGTA‑GAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                   <  2:33137/141‑1 (MQ=255)
                                                                                                                             cggcAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGc               <  2:463808/138‑1 (MQ=255)
                                                                                                                                          gCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa  >  2:659479/1‑141 (MQ=255)
                                                                                                                                          gCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTAACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTTTTGGCTAAGTAGACGGAATACATAATAGGTGACATATAGCCGCACCAATaaaa  >  2:321984/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
AGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAA  >  CP000730/1929709‑1929987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑