Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,010,363 C→T 100% C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,010,3630CT100.0% 164.8 / NA 53C146Y (TGT→TAT) ygaDABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/29);  total (24/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGG  >  CP000730/2010226‑2010494
                                                                                                                                         |                                                                                                                                   
tttCAAGTGTCAAATTTCATAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAAAAAAAAAAAAAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAAt                                                                                                                                  <  2:519328/141‑1 (MQ=255)
     cGTGTCAATTTCCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAAc                                                                                                                             <  1:547830/141‑1 (MQ=255)
        gtCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCaa                                                                                                                          >  2:288728/1‑141 (MQ=255)
               tttttAATCTTCCAAAGAAAACTTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCCGCTAAAGTCAATTTCACATCCAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCa                                                                                                                   <  2:164187/138‑1 (MQ=255)
                 cTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCAtt                                                                                                                 >  1:48662/1‑141 (MQ=255)
                 cTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATATAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCAtt                                                                                                                 >  2:235063/1‑141 (MQ=255)
                    aaTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAc                                                                                                              >  2:114555/1‑141 (MQ=255)
                         tCCAAAGAAAACGTGCACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATATTAATTGTTATATAATCTAACCAAATATTCATTAACGCGGt                                                                                                         <  1:477627/141‑1 (MQ=255)
                         tCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACTGTTCGGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGt                                                                                                         >  1:352816/1‑141 (MQ=255)
                          ccAAACAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGtt                                                                                                        <  1:609330/141‑1 (MQ=255)
                             aaGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGCGCAATAATAATTGTTATATAATCTATCCAAATATTCATTAACCCGGTTaaa                                                                                                     >  1:88171/1‑141 (MQ=255)
                                aaaaCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATg                                                                                                  >  1:478407/1‑141 (MQ=255)
                                   aCGTACACCCTTAAAATGTAAAATGGGAAGATAAACAGTGCTGATAAATGCAATTTCACATATAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCAGTAACCCGGTTAAAATGaaa                                                                                               <  1:61182/141‑1 (MQ=255)
                                       acacCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCtt                                                                                           >  2:495563/1‑141 (MQ=255)
                                         atcGTTAAAATGTAAAAGATGAAGATAAACCGGGCTGCTACAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAAGAATAATTGCTATATAAGCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCtttt                                                                                         <  2:148638/139‑1 (MQ=255)
                                                  atgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCa                                                                                >  1:44538/1‑141 (MQ=255)
                                                    gtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCATTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATAAAATCTTTTGTTTGTTCAAc                                                                              >  1:25567/1‑141 (MQ=255)
                                                    gtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAc                                                                              >  2:349390/1‑141 (MQ=255)
                                                          tgGGAAGATAAACAGTGCTGCTAAAGGCAATTTCACATCTAAAAAGTACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCAtt                                                                        <  2:249736/141‑1 (MQ=255)
                                                             gAAGATAAACGGTGCTGCTAAAGTCAATTTCTCATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAAt                                                                     <  2:64340/141‑1 (MQ=255)
                                                                 aTAAACAGTGCTGCTAAAGTCAATTTTACATCTAAAAATAACATTATGGATAGTGCAATAACAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCAct                                                                 <  2:89815/141‑1 (MQ=255)
                                                                  taattcgtgtTGCTAAATTCAATTTCACATCCAAAAAGAACATTATGGGTAGTGCAATAATAATTGTTATATAATCTTACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCActc                                                                <  2:486478/135‑1 (MQ=255)
                                                                        gTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGata                                                          >  1:560919/1‑141 (MQ=255)
                                                                         ggctgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGatat                                                         <  1:342042/140‑1 (MQ=255)
                                                                          gctgctAAAGTGCATTTCACATCTAAAAAGAACATTATGGATAGTGAAATAATAATTGTTATATAATCTGACCAAATAGTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTGCACCAGCATTAATCACGCTAGATAtt                                                        <  2:251615/141‑1 (MQ=255)
                                                                                aaaGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGa                                                  <  2:584705/141‑1 (MQ=255)
                                                                                     cAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTATTCACTCTAGATATTATTTGACCTAc                                             >  2:543755/1‑141 (MQ=255)
                                                                                          tCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGat                                        >  1:571522/1‑141 (MQ=255)
                                                                                                   aaaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCAt                               <  1:650913/141‑1 (MQ=255)
                                                                                                   aaaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCAt                               >  2:381432/1‑141 (MQ=255)
                                                                                                       aaaCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACTTACTTGATTATTAGCATaaaa                           <  1:346395/140‑1 (MQ=255)
                                                                                                       aaaCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATaaaa                           <  1:646416/140‑1 (MQ=255)
                                                                                                           aTTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtctc                       <  1:108236/141‑1 (MQ=255)
                                                                                                           aTTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtctc                       <  1:196734/141‑1 (MQ=255)
                                                                                                            ttATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTTCTTGACCTACTTGATTATTAGCATAAAATCTCg                      >  2:303348/1‑141 (MQ=255)
                                                                                                            ttATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtctag                      >  2:564096/1‑139 (MQ=255)
                                                                                                              aTGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtctcccc                    >  2:554420/1‑138 (MQ=255)
                                                                                                               tGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCAc                   <  1:88618/141‑1 (MQ=255)
                                                                                                                ggATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACt                  <  1:395791/141‑1 (MQ=255)
                                                                                                                 gATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTTCAGATACTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACtt                 <  2:433632/141‑1 (MQ=255)
                                                                                                                   ttgTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTaa               <  1:303936/139‑1 (MQ=255)
                                                                                                                    aGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTaaa              >  2:522760/1‑141 (MQ=255)
                                                                                                                     gTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTATTTGATTATTAGACTAAAATCTCGCACTTAAAg             >  2:40702/1‑141 (MQ=255)
                                                                                                                       gCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCCAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCt           >  1:81364/1‑141 (MQ=255)
                                                                                                                         aataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg         <  1:235063/141‑1 (MQ=255)
                                                                                                                         aataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg         <  1:288728/141‑1 (MQ=255)
                                                                                                                          ataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGt        >  2:529813/1‑141 (MQ=255)
                                                                                                                            aataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTaa      <  1:114574/141‑1 (MQ=255)
                                                                                                                             ataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTaaa     <  1:199705/141‑1 (MQ=255)
                                                                                                                              taatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAAACTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTGGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAAt    >  1:587637/1‑141 (MQ=255)
                                                                                                                               aatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTCAAtt   >  2:164811/1‑140 (MQ=255)
                                                                                                                                tttgttgTATAATCTTACCAATTATTCATTAACCCTGTTAAAGTGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCGACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATgg  <  2:39599/140‑1 (MQ=255)
                                                                                                                                atTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATgg  <  1:368860/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                   
TTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGG  >  CP000730/2010226‑2010494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑