Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,440,007	Δ1 bp	coding (34/201 nt)	cspA ←	cold shock protein CspA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,440,007	0	C	.	98.5%	293.8 / 0.9	68	coding (34/201 nt)	cspA	cold shock protein CspA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (30/35);  minor base T (0/1);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

TGTGGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCT  >  CP000730/1439865‑1440148                                                                                                                                               |                                                                                                                                              tgtgGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAACATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGtt                                                                                                                                         >  2:742015/1‑148 (MQ=255) tgtgGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGtt                                                                                                                                         >  1:269505/1‑148 (MQ=255)      aCCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAAcc                                                                                                                                    <  1:62885/148‑1 (MQ=255)       ccGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCTGAATCCTTTTT‑AGCGTTAAACCa                                                                                                                                   <  1:589989/148‑1 (MQ=255)       ccGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCa                                                                                                                                   >  2:855659/1‑148 (MQ=255)       ccGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCa                                                                                                                                   <  1:689610/148‑1 (MQ=255)           ggTCGACTTTAACTACTTCAATCTCAGCTGCTTGACCTCCTTCTAATCATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTa                                                                                                                               <  1:168363/148‑1 (MQ=255)             tCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAAc                                                                                                                             >  1:833485/1‑148 (MQ=255)                 tttCAACTTCTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTCCGATAAAGCCGAATCCTTTTT‑AGCGTGAAACCATTTTACTGTa                                                                                                                         <  2:188841/147‑1 (MQ=39)                 ctccAACTACTTCAACCGCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAACGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTa                                                                                                                         <  2:188744/145‑1 (MQ=39)                  ttCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTAc                                                                                                                        <  1:279449/148‑1 (MQ=255)                   tCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTAcc                                                                                                                       >  2:57460/1‑148 (MQ=255)                   tCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACAATTTAACTGTaac                                                                                                                       >  2:189617/1‑146 (MQ=255)                      actactGCAAACCCAACAGCTTGACCTTCTTCTAATGATTTGTAACTATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTg                                                                                                                    <  1:708422/148‑1 (MQ=255)                        tactTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGtt                                                                                                                  <  1:157042/148‑1 (MQ=255)                        tacgTCAATCTCATCAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTCGTCAATTGCTGAAAAATGTACGAATACGTCATTTTCGCCGTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGtt                                                                                                                  <  2:1374/148‑1 (MQ=255)                         actTCAACCTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGttt                                                                                                                 <  1:934155/148‑1 (MQ=255)                          ctTCATACTCATCAGCTTGTCCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTc                                                                                                                <  1:836010/148‑1 (MQ=255)                              aaaCTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATaa                                                                                                            <  1:583382/148‑1 (MQ=255)                                acccAACAGCTTTACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATc                                                                                                          <  1:212407/145‑1 (MQ=255)                                        gCTTGCCCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCTATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCt                                                                                                  <  1:566158/148‑1 (MQ=255)                                          ttGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTcc                                                                                                <  2:358602/148‑1 (MQ=255)                                              ccTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTAGCTTGTTTCATAATCTGAAACCTCCAAGa                                                                                            >  1:565898/1‑148 (MQ=255)                                              ccTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGa                                                                                            >  1:463603/1‑148 (MQ=255)                                                ttcttcTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACt                                                                                          <  1:266720/148‑1 (MQ=255)                                                ttctccTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGAGAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACt                                                                                          <  1:487928/148‑1 (MQ=255)                                                 tcttctAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTa                                                                                         >  2:47758/1‑148 (MQ=255)                                                  cttctAATGATTTGTAACCATCTTGGTTAATTGCTGAACAATGTACGNATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTATAT‑ACCGCTAAACCATTAAACTGTACGTTGTTTCATAATCTGAAACATCCAACACaaa                                                                                        >  2:509621/1‑148 (MQ=255)                                                         tGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcat                                                                                 >  1:88959/1‑148 (MQ=255)                                                          gATTTGTAACCAGCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACAGTACCTTGTTCCATAATCTGAAACCTCCAAAACTAAAattcatt                                                                                >  2:238699/1‑148 (MQ=255)                                                           aTTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGGAAACTCCAAGACTCAAatgcatgc                                                                               >  1:746131/1‑146 (MQ=255)                                                           aTTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACTTCCAAGACTAAAattcatta                                                                               >  1:465192/1‑147 (MQ=255)                                                           aTTTGTAACCATCTTGGTGAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcattc                                                                               >  2:762959/1‑148 (MQ=255)                                                            tttGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAGTCTGAAACTTCCAAGAGTACAGttcattca                                                                              >  1:306507/1‑148 (MQ=255)                                                                      tCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTAt                                                                    <  1:414719/148‑1 (MQ=255)                                                                         tGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCg                                                                 <  1:503779/148‑1 (MQ=255)                                                                         tGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCg                                                                 <  1:742015/148‑1 (MQ=255)                                                                           tttAATTGCTGAAAAATGTACGAACACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCa                                                               <  1:587926/147‑1 (MQ=255)                                                                           gTTAATCGCTGAAAAATGTACGAATACGTCATTTTCCCCCTCAACTCCGATAAAGCCAAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCa                                                               <  1:865079/148‑1 (MQ=255)                                                                              aaTTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCatat                                                            <  2:784208/148‑1 (MQ=255)                                                                                   cTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACaa                                                       <  1:959977/148‑1 (MQ=255)                                                                                   cTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACaa                                                       <  1:22954/148‑1 (MQ=255)                                                                                    tGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACaaa                                                      >  2:788353/1‑148 (MQ=255)                                                                                      aaaaaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACaaaaa                                                    >  2:771929/1‑148 (MQ=255)                                                                                      aaaaaCGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACaaaaa                                                    >  2:675584/1‑148 (MQ=255)                                                                                        aattGTACGAATACTTCCTGTTTTCCTCCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAACCTGACACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAAt                                                  <  2:745950/145‑1 (MQ=255)                                                                                        aaaTGTCCTAATACGTNCCTTTCTCCTTCAACTTCGATAAAGCCGAATCC‑TTTTGAGCGTTACACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAAt                                                  <  1:915593/148‑1 (MQ=255)                                                                                        aaaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAAt                                                  <  1:208968/148‑1 (MQ=255)                                                                                                  aTACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAAt                                        >  1:379556/1‑148 (MQ=255)                                                                                                   tACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAAtt                                       <  1:338029/148‑1 (MQ=255)                                                                                                   tACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAAtt                                       >  1:827698/1‑148 (MQ=255)                                                                                                      gTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCGTTCAATATGCTTATTCGCCTATTACAAAAAATAGATTCTAATTTCa                                    >  2:289958/1‑148 (MQ=255)                                                                                                      gTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCa                                    >  2:18623/1‑148 (MQ=255)                                                                                                       tCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCAc                                   >  1:749319/1‑148 (MQ=255)                                                                                                        cATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTGTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACt                                  >  1:455537/1‑148 (MQ=255)                                                                                                         tttttCTCCTTCAACTTCGATACAGCCGAATCATTTTT‑ATCGTTAATCCATTTAACTGTACCTTGTTTCACAATCTTAATCCTCCAAATCTAAAATTCATTCAATATGCTTATTCTGATATTACAAAAAATACATGCAAATTTCACTc                                 <  2:71320/147‑1 (MQ=255)                                                                                                         aTTTTCTCCTTCAACTTCGATAAAGCCGAATCCTCTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCCCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTc                                 <  2:348320/148‑1 (MQ=255)                                                                                                                  ttCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCt                        >  1:887154/1‑148 (MQ=255)                                                                                                                      aCTTCGATAAAGCCGAATCCTTTTT‑AGCGTTGAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATATCACTCAAATATTCTTTGc                    >  1:633217/1‑148 (MQ=255)                                                                                                                      aCTTCGATAAAGCCGAATCCTTTTT‑AACGCTCCACCAATGAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAACTATTCTTTGc                    >  1:237761/1‑148 (MQ=255)                                                                                                                       cTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAATCCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCa                   <  1:205759/148‑1 (MQ=255)                                                                                                                       cTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCa                   <  1:854074/148‑1 (MQ=255)                                                                                                                       cTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCa                   <  1:70702/148‑1 (MQ=255)                                                                                                                          ggATAAAGCCGAAGCC‑TTTTAAGCGTTAAACCATTTAACTGTATCTCGTTTCATAATCTGAAACCTTCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAata                <  2:157622/147‑1 (MQ=255)                                                                                                                           gATAATGTCGATTCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAatat               <  2:283722/148‑1 (MQ=255)                                                                                                                                     aaTCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAatca     >  2:625760/1‑148 (MQ=255)                                                                                                                                     aaTCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATCCTTTGCAATATGGAATAatca     >  2:452535/1‑148 (MQ=255)                                                                                                                                         ccttttTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCt  <  1:788353/146‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              TGTGGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCT  >  CP000730/1439865‑1440148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑