BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	295,322	G→C	G68A (GGT→GCT)	tarJ →	alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	295,322	0	G	C	99.0%	331.0 / NA	100	G68A (GGT→GCT)	tarJ	alcohol dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (54/44); minor base T (0/1); total (55/45)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGGTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATGGT  >  CP000730/295179‑295464

tGTTGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGaa                                                                                                                                            >  2:150837/1‑148 (MQ=255)

tGTTGATATTTATGGTAATCATGTCATCGTAAGACCTGTATCCTTGTCTATTTGTGCAGCTGATCATAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGATGCGGTTGCTGag                                                                                                                                            >  2:383191/1‑147 (MQ=255)

 gTTGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAg                                                                                                                                           <  1:9046/148‑1 (MQ=255)

   tGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGtt                                                                                                                                         >  1:723962/1‑148 (MQ=255)

      tatTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTGGTa                                                                                                                                      >  2:27028/1‑148 (MQ=255)

      tatTTATGGTAATCATGTCATAGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAAATACCACTGTCAGCAGTTCATGAAACAGTTGAGGAAGTTgaa                                                                                                                                      >  2:748687/1‑146 (MQ=255)

        tttATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGAAtt                                                                                                                                    >  2:561578/1‑148 (MQ=255)

         ttATGGTAAGCTGTTCATTGGAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTAttt                                                                                                                                   <  1:125369/148‑1 (MQ=255)

          tATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGGTGCTGAAGTTGTATTTg                                                                                                                                  >  1:868601/1‑148 (MQ=255)

            tGGTAATCATGTCAACGTAAGACCTTTATACTTGTCTATTAGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACAACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGAt                                                                                                                                <  2:787380/148‑1 (MQ=255)

             ggTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGACGAGATGAAAATGTACTACGCAAAAAATTAACAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGATTTTGATa                                                                                                                               >  1:79842/1‑148 (MQ=255)

             ggTAATCATGTAATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATa                                                                                                                               >  2:856436/1‑148 (MQ=255)

                aaTCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGCATTTGATAGTa                                                                                                                            >  1:44733/1‑148 (MQ=255)

                aaTCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATGAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTa                                                                                                                            >  2:266061/1‑148 (MQ=255)

                 aTCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTaa                                                                                                                           <  1:265642/148‑1 (MQ=255)

                       tCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGt                                                                                                                     <  1:983225/148‑1 (MQ=255)

                         aTCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTAt                                                                                                                   <  1:870173/148‑1 (MQ=255)

                               aGACCTTTATTCTTGTCTATTTGTGCAGCTGATCAACGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGaaa                                                                                                             <  1:551028/148‑1 (MQ=255)

                                gACCTTTATACTTGTCGATTGGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGGGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGaaaa                                                                                                            <  2:251522/148‑1 (MQ=255)

                                 aCCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATTAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGaaaaa                                                                                                           <  2:178292/148‑1 (MQ=255)

                                 aCCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGaaaaa                                                                                                           <  1:113767/148‑1 (MQ=255)

                                   cTTTATACTTGTCTATTTGTGCAGCTGTTCCGAGGTGTTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAgg                                                                                                         >  1:982474/1‑148 (MQ=255)

                                   cTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAgg                                                                                                         >  1:615125/1‑148 (MQ=255)

                                     ttATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTa                                                                                                       >  1:447390/1‑148 (MQ=255)

                                         aCTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTCCGaa                                                                                                   >  2:439139/1‑148 (MQ=255)

                                         aCTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGaa                                                                                                   >  1:119332/1‑148 (MQ=255)

                                         aCTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGATCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTCGAAAAAGGTACGaa                                                                                                   >  1:711092/1‑148 (MQ=255)

                                          cTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGCAAAAGGTACGaac                                                                                                  >  2:244099/1‑147 (MQ=255)

                                            tGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTGGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAACGGGACGAAagt                                                                                                >  1:49276/1‑148 (MQ=255)

                                            tGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAagt                                                                                                >  2:484302/1‑148 (MQ=255)

                                            tGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTGCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAagt                                                                                                >  1:38340/1‑148 (MQ=255)

                                               cTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTAAGAAagtagt                                                                                             >  2:434808/1‑148 (MQ=255)

                                                   ttGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTACAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATg                                                                                         >  2:352956/1‑148 (MQ=255)

                                                   ttGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATg                                                                                         <  1:992488/148‑1 (MQ=255)

                                                          gCTGATCACAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGa                                                                                  <  1:873709/148‑1 (MQ=255)

                                                          gCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGa                                                                                  <  1:136000/148‑1 (MQ=255)

                                                           cTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTGCTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGTAAGTAGTAATGGTACCGaa                                                                                 <  1:702497/148‑1 (MQ=255)

                                                           cTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGGAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGaa                                                                                 <  1:924364/148‑1 (MQ=255)

                                                           cTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGaa                                                                                 <  2:778069/148‑1 (MQ=255)

                                                               tCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATaca                                                                             <  1:565461/148‑1 (MQ=255)

                                                                cAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATacac                                                                            >  1:899683/1‑148 (MQ=255)

                                                                  aaGGTATTACAAAGGTCGAAGAGATTAAAATGTAATACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCt                                                                          <  2:72613/148‑1 (MQ=255)

                                                                    gttATTACATAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGACAGTAGTAATGGTACCGAATACACCTAc                                                                        <  1:530797/146‑1 (MQ=255)

                                                                          acacAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCa                                                                  >  1:31228/1‑148 (MQ=255)

                                                                          acacAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCa                                                                  <  1:435453/148‑1 (MQ=255)

                                                                            acaGGTCGAAGAGATGAAAATGTACTACGCAGAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCaaa                                                                >  2:552709/1‑147 (MQ=255)

                                                                            acaGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATATGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAAc                                                                >  2:650905/1‑148 (MQ=255)

                                                                              aGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAAcat                                                              <  1:810166/148‑1 (MQ=255)

                                                                                    aaGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCAtatt                                                        >  2:739895/1‑148 (MQ=255)

                                                                                       agaTGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGCTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATattatt                                                     <  1:201082/148‑1 (MQ=255)

                                                                                       agaTGAAAATGTACTACGCAAAAAATTAACAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGGGCAACATCATattatt                                                     <  2:364549/148‑1 (MQ=255)

                                                                                         ataaaaaTGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGc                                                   <  2:966251/145‑1 (MQ=255)

                                                                                         aTGAACATGTACTACGCCAAAAATCACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGc                                                   <  2:713692/148‑1 (MQ=255)

                                                                                           gAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACCTCATATTATTGCgg                                                 <  2:637242/148‑1 (MQ=255)

                                                                                                  tactacGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGGAGTTGTATTTGATAGTAAAGGGGTAGTTGAAAAAGGGACGATAGTAGTAATGGTACCGAATACACCTGCAGAGCAACATCATATTATTGCGGAGAATTa                                          <  1:725208/148‑1 (MQ=255)

                                                                                                  tactacGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTa                                          <  2:125705/148‑1 (MQ=255)

                                                                                                   actacGCAAAAAATTACCAATGTTATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTaa                                         >  1:495015/1‑147 (MQ=255)

                                                                                                   actacGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCGGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCGACAGAGCAACATCATATTATTGCGGAGAATTAc                                         >  1:930367/1‑148 (MQ=255)

                                                                                                         cAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACCTCATATTATTGCGGAGAATTACTTAGcc                                   >  2:221229/1‑148 (MQ=255)

                                                                                                         cAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGGGAATTACTTAGcc                                   >  2:179935/1‑148 (MQ=255)

                                                                                                         aaaaaaaTTAGCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACAGAATACAAATACCGAGCACAATCATATTATTGCGGAGAAttgatgagcc                                   >  2:260144/2‑140 (MQ=255)

                                                                                                          aaaaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCct                                  <  1:945443/148‑1 (MQ=255)

                                                                                                           aaaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctc                                 >  2:711565/1‑148 (MQ=255)

                                                                                                           aaaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTAAATTTGATAGTAAAGGGGGATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTAGAGAGCAACATCATATTGTTGCGGAGAATTAATTGGcccc                                 >  2:135212/1‑146 (MQ=255)

                                                                                                            aaaaTTACCAATGTCATTAGTTCATGCACCTCTTGTGGCACTGGTGTTTGTTAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGCATTACTTAGCctct                                <  2:206821/148‑1 (MQ=255)

                                                                                                            aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctct                                <  2:298596/148‑1 (MQ=255)

                                                                                                            aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctct                                >  1:304917/1‑148 (MQ=255)

                                                                                                            aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctct                                >  2:721686/1‑148 (MQ=255)

                                                                                                            aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctca                                >  2:627276/1‑147 (MQ=255)

                                                                                                            aaaaTTACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGGAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCctct                                <  1:333443/148‑1 (MQ=255)

                                                                                                              aaTTCCCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTTTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAg                              <  1:182411/148‑1 (MQ=255)

                                                                                                                ttACCAATGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTGCTTAGCCTCTAGtt                            >  2:919509/1‑148 (MQ=255)

                                                                                                                      aTGGCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGGAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTa                      <  1:38983/148‑1 (MQ=255)

                                                                                                                       tGTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAg                     <  1:677410/148‑1 (MQ=255)

                                                                                                                        ggcATGAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGa                    <  2:62748/146‑1 (MQ=255)

                                                                                                                        gTCATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTata                    >  1:368791/1‑146 (MQ=255)

                                                                                                                          cATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGTGCATTACTTAGCCTCTAGTTATTTTatatt                  >  2:630618/1‑147 (MQ=255)

                                                                                                                          cATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTCTTTTAGATc                  >  1:79393/1‑148 (MQ=255)

                                                                                                                          cATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATc                  >  1:149131/1‑148 (MQ=255)

                                                                                                                          cATTAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTAGTTTAGATc                  >  2:785211/1‑148 (MQ=255)

                                                                                                                            ttAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTa                <  2:31228/148‑1 (MQ=255)

                                                                                                                             tAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAg               >  1:160845/1‑148 (MQ=255)

                                                                                                                             tAGTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAg               >  1:140084/1‑148 (MQ=255)

                                                                                                                               gTTCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTCCTTAGCCTCTAGGTATTTTCGATCTGGTg             >  2:180550/1‑148 (MQ=255)

                                                                                                                                ttCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTgg            <  2:447390/148‑1 (MQ=255)

                                                                                                                                 tCATGACGCTGTTGCTGAAGTTGTATTTGCTAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt           <  1:164614/148‑1 (MQ=255)

                                                                                                                                 tCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt           >  1:328300/1‑148 (MQ=255)

                                                                                                                                 tCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt           >  1:50459/1‑148 (MQ=255)

                                                                                                                                 tCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt           <  1:561578/148‑1 (MQ=255)

                                                                                                                                 tCATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGt           >  1:984748/1‑148 (MQ=255)

                                                                                                                                  cATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGtt          >  2:584499/1‑148 (MQ=255)

                                                                                                                                  cATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGtt          <  2:723962/148‑1 (MQ=255)

                                                                                                                                  cATGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTAATTAGACTCTAGTTATTTTAGGTGTGGTGGtt          >  2:537861/1‑148 (MQ=255)

                                                                                                                                    tGAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTat        >  1:899117/1‑148 (MQ=255)

                                                                                                                                    tGAAGCTGTTGCTGAAGTGGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCGCTAGACAATTTAGGTCTAGTGGTTag        >  2:509247/1‑147 (MQ=255)

                                                                                                                                     gAAGCTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTCGACAAAGGTACGCACGCAGTACTGGCACCGCATCCCCATACAGACCAACATCATATCATTGCGGAGCAGCACGTAGCCTCTAGTGATCTTAGATCTAGTGGTTatg       >  2:577019/1‑148 (MQ=255)

                                                                                                                                         cTGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATgg   <  1:856436/148‑1 (MQ=255)

                                                                                                                                          tGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCATCATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATGGt  <  1:383191/148‑1 (MQ=255)

                                                                                                                                          tGTTGCTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATGGt  <  2:38340/148‑1 (MQ=255)

                                                                                                                                          tGTTGCTGAAGTTGTATTTGATAGTAAAGAGGTATTTGAAAAATGTACTAAAGTAGTAATTGTACCGAATACACCTACATAGCTACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGGTATGATGGt  <  2:49276/148‑1 (MQ=255)

TGTTGATATTTATGGTAATCATGTCATCGTAAGACCTTTATACTTGTCTATTTGTGCAGCTGATCAAAGGTATTACACAGGTCGAAGAGATGAAAATGTACTACGCAAAAAATTACCAATGTCATTAGTTCATGAAGCTGTTGGTGAAGTTGTATTTGATAGTAAAGGGGTATTTGAAAAAGGTACGAAAGTAGTAATGGTACCGAATACACCTACAGAGCAACATCATATTATTGCGGAGAATTACTTAGCCTCTAGTTATTTTAGATCTAGTGGTTATGATGGT  >  CP000730/295179‑295464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`