Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,434,968 G→A D109N (GAT→AAT)  dapD → 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,434,9680GA98.0% 320.0 / NA 99D109N (GAT→AAT) dapD2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (48/49);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACGAAGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCA  >  CP000730/1434826‑1435111
                                                                                                                                              |                                                                                                                                               
ttACGAAGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAGGCCATTATTGAAGATggg                                                                                                                                            >  1:778248/1‑147 (MQ=255)
  aCGAATCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGc                                                                                                                                          <  1:261032/148‑1 (MQ=255)
  aCGAAGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGc                                                                                                                                          <  1:989915/148‑1 (MQ=255)
    gAAGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTg                                                                                                                                        >  2:48395/1‑148 (MQ=255)
      aGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTc                                                                                                                                      <  2:204997/148‑1 (MQ=255)
      aGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACAAGGTGCGTTAATTAGAGAACAAGCCATAATTGAAAatgatgagatg                                                                                                                                      >  2:768725/1‑143 (MQ=255)
       gCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATCAGAGAACAAGCCATTATTGAAAATGGTGCTGTCg                                                                                                                                     <  2:120004/148‑1 (MQ=255)
        cATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTAGt                                                                                                                                    >  1:746858/1‑148 (MQ=255)
             ggTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACGAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTatga                                                                                                                               >  1:382889/1‑148 (MQ=255)
              gTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTatgat                                                                                                                              >  2:430947/1‑148 (MQ=255)
              gTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTatgat                                                                                                                              >  1:445924/1‑148 (MQ=255)
               tAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTatgatg                                                                                                                             >  2:446948/1‑148 (MQ=255)
               tAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTatgatg                                                                                                                             <  2:778409/148‑1 (MQ=255)
                aGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTCGAGAACAAGCCATTATTGCAGATGCTCTTGTCGTTTTGATgg                                                                                                                            >  2:932819/1‑148 (MQ=255)
                aGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATgg                                                                                                                            >  1:57505/1‑148 (MQ=255)
                     aTTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACACGCCATTCTTGAAAATGGTGCTGTCGTGATGATGGGCGca                                                                                                                       >  1:257949/1‑148 (MQ=255)
                     aTTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTatgatcgcggca                                                                                                                       >  1:222117/1‑141 (MQ=255)
                     aTTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGca                                                                                                                       >  2:461797/1‑148 (MQ=255)
                     aTTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCAGTCGTTATGATGGGCGca                                                                                                                       >  1:228181/1‑148 (MQ=255)
                        cGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGcaaca                                                                                                                    <  1:465426/148‑1 (MQ=255)
                        cGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGcaaca                                                                                                                    <  2:812628/148‑1 (MQ=255)
                         gAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGcaacaa                                                                                                                   <  2:935927/148‑1 (MQ=255)
                              tataGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAat                                                                                                              >  1:813342/1‑148 (MQ=255)
                              tataGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAat                                                                                                              >  2:866353/1‑148 (MQ=255)
                              tataGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATCCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAGTTAat                                                                                                              >  1:375932/1‑148 (MQ=255)
                                  gAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTg                                                                                                          >  1:748229/1‑148 (MQ=255)
                                    aaatGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTATAAAATACGAATGCACGAATTGAACGAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGgc                                                                                                        <  1:766656/145‑1 (MQ=255)
                                     aTTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGgcg                                                                                                       >  2:223261/1‑148 (MQ=255)
                                     aTTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGgcg                                                                                                       >  2:920500/1‑148 (MQ=255)
                                        gAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAg                                                                                                    >  1:320159/1‑148 (MQ=255)
                                        gAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCCCAg                                                                                                    >  1:735527/1‑148 (MQ=255)
                                         aaaTGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGt                                                                                                   >  1:155871/1‑148 (MQ=255)
                                         aaaTGGATCGTCGCAATTATGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGt                                                                                                   >  1:142990/1‑148 (MQ=255)
                                            tgtaTCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGt                                                                                                <  1:719577/145‑1 (MQ=255)
                                              gATCGTCGCAATTCTGCTATTCCATTCAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTg                                                                                              >  1:646066/1‑148 (MQ=255)
                                              gATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTg                                                                                              >  2:249659/1‑148 (MQ=255)
                                                 cgtcgCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCg                                                                                           <  1:48395/148‑1 (MQ=255)
                                                 cgtcgCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCg                                                                                           <  1:285087/148‑1 (MQ=255)
                                                     gccaTTCTAGTATTCCATTAAAAGACTTAACAAATACGAATGCAGCGATTGCACCAGGTGCGTTTATTAGAGGACAAGCCATCATTGTAAATGGTGCTGTCGTTATGATGGGCGCTACAATTAATATGGGCGCAGTCGTTGGCGAAgg                                                                                       <  2:396423/145‑1 (MQ=255)
                                                      taactCTGCTATTCCATTAAAAGAATTGTCAATTACGAATGCACGAATTGAACCAGGTGCTTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGt                                                                                      <  1:668126/144‑1 (MQ=255)
                                                      cAAGTCTGCTATTCCATGAAAAGACTTAACAAATACCAATGCACGAATTGAACCAGGTGCGTTTATTACAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGt                                                                                      <  1:848010/148‑1 (MQ=255)
                                                             gCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAAtga                                                                               <  1:739061/148‑1 (MQ=255)
                                                              cTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAAtgat                                                                              >  1:701234/1‑148 (MQ=255)
                                                                aTTCCATTAAAAGACTTAACAAATACTAATGCACGAATTGCACCAGGTGCGTTTATTATAGAACAAGCCATTATTGAAAATGGTGCCGTCTTTATGATGGGCGAAAAAAATAAAATTGTCACGGTCGTTGGCGATGGCATAAttcttt                                                                            <  2:259242/148‑6 (MQ=255)
                                                                aTTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAAtgattg                                                                            <  2:23732/148‑1 (MQ=255)
                                                                aTTCCATTAAAAGACTTAAAAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGAGGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAAtgattg                                                                            <  2:357130/148‑1 (MQ=255)
                                                                 ttCCATTAAAAGATTTAACAAATACGAATGCACGAATTGAACCAGGTGCCTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAAtgattga                                                                           <  1:768725/148‑1 (MQ=255)
                                                                 ttCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAAtgattga                                                                           <  1:988851/148‑1 (MQ=255)
                                                                     aTTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAAGGATTGATATg                                                                       >  1:118634/1‑148 (MQ=255)
                                                                      ttAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGa                                                                      <  1:691699/148‑1 (MQ=255)
                                                                       tAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGaa                                                                     <  2:293883/148‑1 (MQ=255)
                                                                       tAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAAGGATTGATATGaa                                                                     >  1:903273/1‑148 (MQ=255)
                                                                       tAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATAGTGGCGCAGGCGTTGGCGAAGGTACAATGATTGCTATGaa                                                                     >  1:764810/1‑148 (MQ=255)
                                                                        aaaaGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAAt                                                                    <  2:940144/148‑1 (MQ=255)
                                                                        aaaaGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAAt                                                                    >  2:309889/1‑148 (MQ=255)
                                                                        aaaaGACTTAACAAATACGAAGGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAAt                                                                    <  2:512275/148‑1 (MQ=255)
                                                                        aaaaGAATTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGCATAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCGACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAAt                                                                    <  2:547612/148‑1 (MQ=255)
                                                                          aaGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTCTTGAAAATGGTGCTGTCGTTATGATGGGAGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGCGATGAAGGc                                                                  >  1:263214/1‑148 (MQ=255)
                                                                           aGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCt                                                                 >  2:820071/1‑148 (MQ=255)
                                                                           aGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCt                                                                 >  2:350182/1‑148 (MQ=255)
                                                                              cTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTAct                                                              >  2:417490/1‑148 (MQ=255)
                                                                                  aCAAATACGAGTGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATACTGGCGCAGTCGTTGGCGAAGGTAGAATGATGGATATGAATGCTACTCTCg                                                          <  2:746858/148‑1 (MQ=255)
                                                                                  aCAAATACGAAGGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCg                                                          <  1:530710/148‑1 (MQ=255)
                                                                                     aaTACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCggtg                                                       >  2:230228/1‑148 (MQ=255)
                                                                                         taaaTGCAAGAATTGAACCAGGGAGGTTTGTCAAATAAAAAGGTATTATTTAAATTTGTGCTGTCGTTATGATGGGCGTAATAATTAATATTGGCGCAGTAGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCg                                                   <  2:646959/146‑1 (MQ=255)
                                                                                          taaattaaagaataaaCCAGGTGCGTTTATAAGAGAACAACCAAGTATTTAAAATGGTGCTGTCGTCATGATGGGCGCAACACTTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGt                                                  <  2:241389/134‑1 (MQ=255)
                                                                                          gAATGTACTAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTACTCTTGCATCTATTGTTGTTGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGt                                                  <  1:774310/148‑1 (MQ=255)
                                                                                          gAATGCACGAATTGGACCGGGTGCGTTTATTGGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGt                                                  <  1:7999/148‑1 (MQ=255)
                                                                                          gAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCgc                                                  >  2:403393/1‑147 (MQ=255)
                                                                                           aaTGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGtc                                                 >  2:808627/1‑147 (MQ=255)
                                                                                           aaTGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTg                                                 >  2:319829/1‑148 (MQ=255)
                                                                                                aCGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGGTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCgacgtgta                                            >  2:634585/1‑141 (MQ=255)
                                                                                                 cGAATTGAACCAGGGGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACaa                                           <  1:820635/148‑1 (MQ=255)
                                                                                                  gAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCgccgtaccat                                          >  2:859904/1‑139 (MQ=255)
                                                                                                  gAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAAc                                          >  1:852407/1‑148 (MQ=255)
                                                                                                  gAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAAc                                          <  1:552073/148‑1 (MQ=255)
                                                                                                  gAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGACGCCCTACaaa                                          >  2:20313/1‑147 (MQ=255)
                                                                                                  gAATTGAACCAGGTGCGTTTATTACAGAACAAGCCATGTCTGAAAAGGGTGCTGTCGTTATGAGGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAAc                                          <  1:671661/148‑1 (MQ=255)
                                                                                                      tGAACCAGGTGCGTTTATTAGAGAACGAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGTAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGt                                      <  2:305335/148‑1 (MQ=255)
                                                                                                      tGAACCAGGTGCGTTTATTAGAGAACAATCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGt                                      <  1:563686/148‑1 (MQ=255)
                                                                                                          ccAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTaaaa                                  <  1:900046/148‑1 (MQ=255)
                                                                                                              gTGCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGt                              <  1:866353/148‑1 (MQ=255)
                                                                                                               tGCGTTTATTAGACAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTa                             <  1:83364/148‑1 (MQ=255)
                                                                                                                gCGTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTAc                            >  1:61340/1‑148 (MQ=255)
                                                                                                                 ggTTTATTAGAGAAAAAGCCATTCTTGAAAATGGTGCGGTCTTTATGATTGGCGCAACAATTAATATTGCCGGAGTCGTTGGCGAAGGTACAATGATTGATTTGCATGCTACTCTCGGGGGTCGGGCTACAACTGGTAAAAATGTACa                           <  2:778248/147‑1 (MQ=255)
                                                                                                                  gTTTATTAGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATGGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACAt                          <  2:813342/148‑1 (MQ=255)
                                                                                                                  gTTTATTAGAGAACAAGCAATTATGGAAAATGGTGCTGTCGTTATTATGGGTGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACAt                          <  2:941730/148‑1 (MQ=255)
                                                                                                                  gTTTATAAGAGACCATGCCATTATTGAAAAGGGTGCTGTCGTTATGATCGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACCATGATTGATATGCATGCTACTCCCGGTGGCCGTGCTACAACTGGTAAAAATGTACAt                          <  1:621443/148‑1 (MQ=255)
                                                                                                                  ctttATTAGAGACAAAGCCATTATTTGAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGTTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACAt                          <  1:195840/147‑1 (MQ=255)
                                                                                                                  cttatttaGAGAACAAGCCATTAATGAAAATGGTGCTGTCTCTATTGTGGGGGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACAt                          <  2:375932/143‑1 (MQ=255)
                                                                                                                        taGAGAACAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAggg                    >  1:666512/1‑148 (MQ=255)
                                                                                                                             aaCAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCGGTACCACTAGAAAAAAAATACATGTAGGGGCggg               >  2:780131/1‑148 (MQ=255)
                                                                                                                              acaagtaattgctGAAAATGGTGCTGTTGTTAGGATGGGCGCAACAATTTATATTAGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGgc              <  2:630009/136‑1 (MQ=255)
                                                                                                                              aCAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGCCCTATCATTAATAAAAAAATACATGTAGGGGCAGgc              >  2:791479/1‑148 (MQ=255)
                                                                                                                               cAAGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGgcg             <  1:309889/148‑1 (MQ=255)
                                                                                                                                aagtttgtattGCAAATGGTGGTGGTGTTATGATGGGCGGAAGAATTAATACTGGCGCAGTCTCGGGGGTAGGGATAATGATTGAGATGAATGCTTCTGTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGgtgc            <  2:413315/141‑1 (MQ=255)
                                                                                                                                aaGCCATTATTGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGgcgc            >  1:748190/1‑148 (MQ=255)
                                                                                                                                          tGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTAAATGTAGGGGCTGGCGCAGTATTAGCa  >  1:415940/1‑148 (MQ=255)
                                                                                                                                          tGAAAATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCCTTCTTCTTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCa  >  1:228580/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                               
TTACGAAGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCA  >  CP000730/1434826‑1435111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: