BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,759,125	G→A	Q57* (CAA→TAA)	USA300HOU_1650 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,759,125	0	G	A	98.1%	320.4 / NA	108	Q57* (CAA→TAA)	USA300HOU_1650	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (55/48); minor base C (1/1); total (59/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AGCCATGCGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTGGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCTATAATGTTTTT  >  CP000730/1758982‑1759269

aGCCATGCGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGca                                                                                                                                              >  1:930069/1‑148 (MQ=255)

 gCCATGCGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACCTTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGcac                                                                                                                                             >  1:29418/1‑147 (MQ=255)

 gCCATGCGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTACATCAGTTTCTGTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGcat                                                                                                                                             >  2:742048/1‑148 (MQ=255)

  ccATGCGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGcatc                                                                                                                                            >  2:439653/1‑148 (MQ=255)

   cATGCGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGcatca                                                                                                                                           >  1:572948/1‑148 (MQ=255)

   cATGCGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGcatca                                                                                                                                           >  1:954345/1‑148 (MQ=255)

      gCGATTCATTCCAGACACAGCCATCACCTGTTGTTAATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACTTTCTTTATTATATCATTTTCTTTTATTGAATATTTCTCACTACTTACTTAAATATT‑TTTGGCATCAttg                                                                                                                                        >  1:730722/1‑147 (MQ=255)

       cGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAg                                                                                                                                       >  2:668045/1‑148 (MQ=255)

       cGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑GTAGGCATCATTAg                                                                                                                                       >  1:21893/1‑148 (MQ=255)

         attcattcCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACGTTGTTTATTAAATCCTTTTCTTTTAATGAATATTTTTAGCCACTTACATTAACTTG‑TTTGGCCTCATTAGtt                                                                                                                                     >  2:899910/1‑147 (MQ=255)

         attcattcCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTAACCACTTACATAAACATT‑TTAGTCATTATTTGtt                                                                                                                                     >  2:223118/1‑147 (MQ=255)

              ttcCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTg                                                                                                                                >  2:514647/1‑148 (MQ=255)

                ccAGACACAGCCATAAAATCTTGTTTATCTAACATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGtgtcg                                                                                                                              >  1:632680/1‑144 (MQ=255)

                  aGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTa                                                                                                                            >  2:367158/1‑148 (MQ=255)

                  aGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGCGTCGCGTa                                                                                                                            >  2:45826/1‑148 (MQ=255)

                     cgtaGCCATGAGCTCGTGGTTATCTATCAGGTTAAAGACATGTTTAATTTTGCTTAATGGTACATTCTTTAGTAAATCATTTTCGTTTAAGTAATATTTCTCACCACTTACATAAACGTG‑TTAGGCATCATTAGTGGGTTGCGTAACt                                                                                                                         <  1:448415/145‑1 (MQ=255)

                       caGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAGTATTTTTCTGTACTTACAT‑AATATGATTAGGCATCATTTTTGGGTTGCGTACCttt                                                                                                                       >  2:451212/1‑147 (MQ=255)

                         gCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATTATTGGTGGGTTGCGTAACTTGAt                                                                                                                     >  1:261289/1‑148 (MQ=255)

                         gCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAAAATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGCGTTGCGTAACTTGAt                                                                                                                     <  2:730722/148‑1 (MQ=255)

                            aTAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCa                                                                                                                  <  2:868197/148‑1 (MQ=255)

                               aaCTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCAttt                                                                                                               >  2:707264/1‑148 (MQ=255)

                               aaCTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCAttt                                                                                                               <  1:921818/148‑1 (MQ=255)

                               aaCTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCAttt                                                                                                               <  1:854133/148‑1 (MQ=255)

                                 ctctTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGa                                                                                                             >  2:81177/1‑148 (MQ=255)

                                         tatctatcATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATc                                                                                                     >  1:334396/1‑148 (MQ=255)

                                             tatcATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGa                                                                                                 <  2:270172/148‑1 (MQ=255)

                                              atcATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGAt                                                                                                <  2:247301/148‑1 (MQ=255)

                                                 aTTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTa                                                                                             <  1:336468/148‑1 (MQ=255)

                                                 aTTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGAATCATTATTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTTATTTa                                                                                             >  2:15308/1‑148 (MQ=255)

                                                    ttAAATACATTTTTTTTTTTTTTTTATGGTACATTCTTTATTAAATCATTTTATTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGTTTTTTGTAACTTGATTCATTTGATCTTTATCGTTTTTTACAg                                                                                          >  2:132520/1‑148 (MQ=255)

                                                    ttAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAg                                                                                          >  2:66495/1‑148 (MQ=255)

                                                    ttAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGAGTAAATTGATTCATTTGATCATTATCGTGATTTACAg                                                                                          >  1:131547/1‑148 (MQ=255)

                                                            ttttttAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCaa                                                                                  <  1:157039/147‑1 (MQ=255)

                                                            aTTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCaa                                                                                  >  1:467654/1‑148 (MQ=255)

                                                            aTTTTTAATTTGACTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCaa                                                                                  <  1:899666/148‑1 (MQ=255)

                                                               tttAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCt                                                                               <  1:125217/148‑1 (MQ=255)

                                                               tttAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTATAAACAATTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATAATTATCGTGATTTAAAGGATTTCAATCt                                                                               >  2:899811/1‑148 (MQ=255)

                                                               tttAATTTGGCTTAATGGTACATTCTTTATTAAATAATTTTCTTTTAATGAATATTTATCACAACTTATATAATTTTG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATTATTATAGTGATTTACAGGATTCCAAtgt                                                                               >  2:899839/1‑146 (MQ=255)

                                                                ttAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTCGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCtt                                                                              <  2:568417/148‑1 (MQ=255)

                                                                ttAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCtt                                                                              <  1:9811/148‑1 (MQ=255)

                                                                ttAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCtt                                                                              <  2:419702/148‑1 (MQ=255)

                                                                      tGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCtt                                                                        <  1:81177/148‑1 (MQ=255)

                                                                       ggCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCttt                                                                       >  1:584599/1‑148 (MQ=255)

                                                                         cTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTa                                                                     <  1:653108/148‑1 (MQ=255)

                                                                         cTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTa                                                                     <  1:404510/148‑1 (MQ=255)

                                                                            aaTGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGTTTTACAGGATTCAAATATTGTTCTTTTacaa                                                                  >  2:624896/1‑148 (MQ=255)

                                                                            aaTGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTaaaa                                                                  >  1:509566/1‑148 (MQ=255)

                                                                             aTGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTaaaaa                                                                 <  2:734194/148‑1 (MQ=255)

                                                                               ggTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAg                                                               <  2:334396/148‑1 (MQ=255)

                                                                                  aCATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTg                                                            <  1:108548/148‑1 (MQ=255)

                                                                                  aCATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTg                                                            <  2:426387/148‑1 (MQ=255)

                                                                                  aCATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTg                                                            <  1:113946/148‑1 (MQ=255)

                                                                                       cTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTAt                                                       <  1:266107/148‑1 (MQ=255)

                                                                                       cTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTAt                                                       <  1:899098/148‑1 (MQ=255)

                                                                                        tttATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTAtt                                                      >  1:339876/1‑148 (MQ=255)

                                                                                        tttATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTAtt                                                      >  1:326007/1‑148 (MQ=255)

                                                                                        tttATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTAtt                                                      >  2:290741/1‑148 (MQ=255)

                                                                                        tttATAAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTAtt                                                      >  1:692119/1‑148 (MQ=255)

                                                                                             taAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAg                                                 <  1:891837/148‑1 (MQ=255)

                                                                                             taAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAg                                                 <  1:495625/148‑1 (MQ=255)

                                                                                              aaaTCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAgg                                                >  2:780897/1‑148 (MQ=255)

                                                                                                 tCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGa                                             >  1:382880/1‑148 (MQ=255)

                                                                                                   aTTTTCTTTTAATGAATATTTCTCACCACTTACATAAACTTG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGata                                           <  2:310600/148‑1 (MQ=255)

                                                                                                    ttttCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGatat                                          >  2:558410/1‑148 (MQ=255)

                                                                                                     tttCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGCTCATTATCGTGATTTACAGGATTCCACTCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGAtata                                         >  1:421386/1‑148 (MQ=255)

                                                                                                      ttCTTTTAATGGATATTTCTTCCCACTTACATGAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGCTTCATTTGATCCTTATCGTGATGTACTGGCTTCCAATCTTGTTCTTTTAAATAAGGTGCGTATTTCAAGGCGATATaa                                        <  2:23485/148‑1 (MQ=255)

                                                                                                      ttCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATaa                                        <  2:518042/148‑1 (MQ=255)

                                                                                                        cTTTTACTGAATATTTCTCACCACTTACTTAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAAc                                      <  2:191366/148‑1 (MQ=255)

                                                                                                        cTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAAc                                      <  2:584599/148‑1 (MQ=255)

                                                                                                                gAATATTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTAtac                              >  1:222792/1‑146 (MQ=255)

                                                                                                                   tatTTCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGGGTATTTCAAGGCGATATAAACAATTATCACaa                           >  1:825650/1‑148 (MQ=255)

                                                                                                                     tttCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAAtt                         <  1:788271/148‑1 (MQ=255)

                                                                                                                      ttCTCCTCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGAATGACTCGCTTCAATTGTGCCTTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTg                        <  2:209115/148‑1 (MQ=255)

                                                                                                                      ttCTCACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTg                        <  1:707264/148‑1 (MQ=255)

                                                                                                                        ctcACCACTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCt                      <  1:439653/148‑1 (MQ=255)

                                                                                                                          caccacTTACATAAACATG‑TTCGGCAACCTCCGTGGGTTGCGTAACTTGATTGAGTTGATAATAAACGCGGTGTACAGGACTCCAAGCTTGTTCTCTCATAAAAGCAGCGTATTTCAAGGCGCCATCAACAATTATCACAATAGCTAt                    >  2:647961/1‑148 (MQ=255)

                                                                                                                          caccacTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGCTTCATTTGATCATTATCGGGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTAt                    >  2:162569/1‑148 (MQ=255)

                                                                                                                          caccacTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTAt                    >  1:18485/1‑148 (MQ=255)

                                                                                                                           accacTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTACCTTGATTCCTTTGATCGTTATCGTGATTTACAGGTTTCCAATTTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTAtt                   >  1:197805/1‑148 (MQ=255)

                                                                                                                           accacTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGAGTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTAtt                   >  1:384044/1‑148 (MQ=255)

                                                                                                                           accacTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGAGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTAtt                   >  2:937087/1‑148 (MQ=255)

                                                                                                                             catatACATAAACATT‑GTAGGAATCATTAGTGGTTTGAGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAATGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTAc                 <  2:940951/144‑1 (MQ=255)

                                                                                                                             cacTTACATTAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATCCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTAc                 <  2:689899/148‑1 (MQ=255)

                                                                                                                              atttACATAAACATG‑TTAGGCATCATCAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTAACACAATTGCTATTACt                <  1:728443/146‑1 (MQ=255)

                                                                                                                              acTTACCTTAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACt                <  2:238527/148‑1 (MQ=255)

                                                                                                                              acTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACt                <  2:413046/148‑1 (MQ=255)

                                                                                                                               cTTACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTg               >  1:173541/1‑148 (MQ=255)

                                                                                                                                 tACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCt             <  1:899839/148‑1 (MQ=255)

                                                                                                                                 tACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCt             <  1:244929/148‑1 (MQ=255)

                                                                                                                                 tACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCt             <  1:899811/148‑1 (MQ=255)

                                                                                                                                 tACATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCt             <  1:579261/148‑1 (MQ=255)

                                                                                                                                  aCATAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCta            <  1:420443/148‑1 (MQ=255)

                                                                                                                                    aTAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCtata          >  2:650908/1‑148 (MQ=255)

                                                                                                                                    aTAAACATG‑TTAGGCATCATTAGTGGGCTGCGTAACTTGATTCATTTGATCGTTATCGTGATTTAGAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGAGATAAACAGTTATCACAATGGCTATTACTGCtata          >  1:602677/1‑148 (MQ=255)

                                                                                                                                     tAAACATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATATGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAAAAATTATCACAATGGCTATTACTGCTGTaa         >  2:66793/1‑148 (MQ=255)

                                                                                                                                       aaCATG‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTAATGCtaacatt       >  2:401579/1‑143 (MQ=255)

                                                                                                                                        aCATG‑TTAGGCATCATTAGTGGGTTGCGTCACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAGTCTTGTTCTTTTAAAAAAGGAGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCTATAATGt      >  2:444277/1‑148 (MQ=255)

                                                                                                                                        aCATG‑TTAGGCATCATTAGGGGGTTGCGTAACTTGATTCATTTGATCATTATCGGGATTTACAGGATTCCAATCGTGTTCTTTTAAAAAAGGTGAGTATTTCAAGGCGATAGAAACAATTATAACATTTGCTAttcatccatttatgt      >  2:29085/1‑135 (MQ=255)

                                                                                                                                         cATG‑TTAGGCATCATTAGTGGGTTACGTAACTTGATTCATTAGATCATTATCGTGTTTTATAGGATGTAAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCTATAATGtt     >  2:950997/1‑148 (MQ=255)

                                                                                                                                            g‑TTAGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCTATAATGttttt  <  2:339876/148‑1 (MQ=255)

AGCCATGCGATTCATTCCAGACACAGCCATAAACTCTTGTTTATCTATCATTTTAAATACATTTTTAATTTGGCTTAATGGTACATTCTTTATTAAATCATTTTCTTTTAATGAATATTTCTCACCACTTACATAAACATG‑TTGGGCATCATTAGTGGGTTGCGTAACTTGATTCATTTGATCATTATCGTGATTTACAGGATTCCAATCTTGTTCTTTTAAAAAAGGTGCGTATTTCAAGGCGATATAAACAATTATCACAATTGCTATTACTGCTATAATGTTTTT  >  CP000730/1758982‑1759269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`