Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	657,532	G→A	M29I (ATG→ATA)	USA300HOU_0596 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	657,532	0	G	A	98.9%	304.4 / NA	92	M29I (ATG→ATA)	USA300HOU_0596	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (29/62);  minor base T (0/1);  total (29/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGATTAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATGTTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCA  >  CP000730/657395‑657675                                                                                                                                          |                                                                                                                                                ttGATTAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGc                                                                                                                                       >  2:52371/1‑148 (MQ=255)  tGATTAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCt                                                                                                                                      <  2:863421/148‑1 (MQ=255)   gATTAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCtt                                                                                                                                     <  2:673996/148‑1 (MQ=255)   gATTAACTTAGATAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCtt                                                                                                                                     <  2:885449/148‑1 (MQ=255)    aTTAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTc                                                                                                                                    <  1:74276/148‑1 (MQ=255)    aTTAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTc                                                                                                                                    <  2:869531/148‑1 (MQ=255)    aTTAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTc                                                                                                                                    <  2:321449/148‑1 (MQ=255)      tAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCaa                                                                                                                                  <  2:72923/148‑1 (MQ=255)      tAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCaa                                                                                                                                  <  2:442834/148‑1 (MQ=255)      tAACTTAGGTAGTATCGGATAATTAAATGTTGTTTCATAAAAAGCAATGATTTTATATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCaa                                                                                                                                  <  2:919654/148‑1 (MQ=255)       aaCTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCaaa                                                                                                                                 >  1:271492/1‑148 (MQ=255)          ttAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATaa                                                                                                                              >  2:563423/1‑148 (MQ=255)              gtagtaTCGGATACTTACATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATaaaaaa                                                                                                                          <  1:797152/148‑1 (MQ=255)              gtagtaTCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATaaaaaa                                                                                                                          <  1:220940/148‑1 (MQ=255)               tagtaTCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAaat                                                                                                                         >  2:539994/1‑148 (MQ=255)                agtaTCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAaata                                                                                                                        <  2:432723/148‑1 (MQ=255)                agtaTCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAaata                                                                                                                        >  2:633223/1‑148 (MQ=255)                agtaTCGGATACTTAAATGTTGGATCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAaata                                                                                                                        >  1:497425/1‑148 (MQ=255)                agtaTCGGATACTTAAATGTTCGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAaata                                                                                                                        <  2:906694/148‑1 (MQ=255)                agtaTCGGAGACTTAAATATTAATAAAAAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAaata                                                                                                                        <  2:473804/148‑1 (MQ=255)                   aTCGGATACTTAAATGTTAGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAAtt                                                                                                                     <  2:86801/148‑1 (MQ=255)                            ttAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAgg                                                                                                            <  1:328083/148‑1 (MQ=255)                            ttAAATGGTTGTGCATAAAAACGAATGAATTTAAATCGAGTATCTAACTTAGAACAGTGAAATAAAACAGCCAAAGTTATAAAAAGGTAATTATGAACTAAGTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAgg                                                                                                            <  2:28665/148‑1 (MQ=255)                             tAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGt                                                                                                           <  2:357652/148‑1 (MQ=255)                              aaaTGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTg                                                                                                          >  1:805868/1‑148 (MQ=255)                                aTGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAACAACGCTTCAACTAAAAAATAATTAGAATAAAGgggg                                                                                                        >  1:715978/1‑148 (MQ=255)                                 tGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGa                                                                                                       >  2:850074/1‑148 (MQ=255)                                 tGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGa                                                                                                       >  1:484700/1‑148 (MQ=255)                                        tCATAAAAAGCAATGATTATAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATaa                                                                                                <  2:416152/148‑1 (MQ=255)                                            aaaaaGCAATGCTTTTAAATCGAGGAGCTATCTTAGAACAGCGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAAATAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCaa                                                                                            <  2:306011/148‑1 (MQ=255)                                            aaaaaGCAATGATTTTACATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCaa                                                                                            >  2:462411/1‑148 (MQ=255)                                            aaaaaGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCaa                                                                                            >  1:55075/1‑148 (MQ=255)                                            aaaaaGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCaa                                                                                            <  1:906229/148‑1 (MQ=255)                                              aaaTCAATGATTTTAAAACGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATg                                                                                          <  2:488715/148‑1 (MQ=255)                                                aGCAAAGATTATAAAGCGAGGAGCTATCTTAAAAAAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAACAACTAATTAAATTATATTAGCCACGCCTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCt                                                                                        <  2:2986/148‑1 (MQ=255)                                                 gCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTa                                                                                       <  1:905065/148‑1 (MQ=255)                                                  cAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTAt                                                                                      >  2:441327/1‑148 (MQ=255)                                                  cAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTAt                                                                                      >  2:335007/1‑148 (MQ=255)                                                  cAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTAt                                                                                      >  2:958472/1‑148 (MQ=255)                                                  cAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTAt                                                                                      >  2:964548/1‑148 (MQ=255)                                                  cAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTAt                                                                                      >  2:903360/1‑148 (MQ=255)                                                    aTGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGc                                                                                    <  2:271492/148‑1 (MQ=255)                                                     tGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCg                                                                                   >  2:163540/1‑148 (MQ=255)                                                      taTTATAAATCGAGGAGCTATCTTAGAAGAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGa                                                                                  <  2:538600/147‑1 (MQ=255)                                                      gATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGa                                                                                  <  1:462934/148‑1 (MQ=255)                                                      gATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGa                                                                                  <  1:859096/148‑1 (MQ=255)                                                      gATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCAAAATACAAAAAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGa                                                                                  <  2:839339/148‑1 (MQ=255)                                                          ttAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAACAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCt                                                                              <  2:179091/148‑1 (MQ=255)                                                                cGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAatc                                                                        <  2:805868/148‑1 (MQ=255)                                                                cGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAatc                                                                        <  1:731433/148‑1 (MQ=255)                                                                   ggagCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAatcatc                                                                     >  2:701322/1‑148 (MQ=255)                                                                    gagCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACCAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAAtcatca                                                                    <  2:265283/148‑1 (MQ=255)                                                                          tCTTAGAACAGGGAAATAAAACAGCTAAAGTTATAAAAAGTGTTTTTATAACTAATTATATTATTTTAGCCACAATAAAAAAAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATaaaa                                                              <  2:845563/148‑1 (MQ=255)                                                                          tCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATaaaa                                                              <  1:642399/148‑1 (MQ=255)                                                                          tCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATaaaa                                                              <  1:398374/148‑1 (MQ=255)                                                                          tCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATaaaa                                                              <  2:226981/148‑1 (MQ=255)                                                                            ttAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAAc                                                            <  1:109178/148‑1 (MQ=255)                                                                            ttAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAAc                                                            <  1:865458/148‑1 (MQ=255)                                                                             tataaCAGGCAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAAcc                                                           <  2:127864/145‑1 (MQ=255)                                                                             tAGAACAGGTAAATAAAACAGCAAAAGTTACAAAAAGTGAATTAATAACTAAGTATATTATATTAGCCACGCTTCAAAGAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAAcc                                                           <  2:683161/148‑1 (MQ=255)                                                                              aGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAAccc                                                          >  1:505181/1‑148 (MQ=255)                                                                               gAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAAcccc                                                         <  1:397226/148‑1 (MQ=255)                                                                               gAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAAcccc                                                         >  1:209094/1‑148 (MQ=255)                                                                                aagaGGGAAATAAAACATCCAAAGTTATAAAAAGTGAATTAATAACTAATTAGATAATAGTAGCCATGTTTCAAAGAAAAAATAATTAGAATTAGGTGGTATTGATAATCAATGCTATGCCAATCTAAAATCCTCAATAAAATccccc                                                        <  2:44226/145‑2 (MQ=255)                                                                                aaCAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCa                                                        <  1:42902/148‑1 (MQ=255)                                                                                aaCAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCa                                                        <  1:888098/148‑1 (MQ=255)                                                                                 aCAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCaa                                                       <  2:6616/148‑1 (MQ=255)                                                                                  cAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCaaa                                                      <  1:40580/148‑1 (MQ=255)                                                                                     ggAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAatat                                                   >  2:991457/1‑148 (MQ=255)                                                                                       aaaTAAACCATCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAg                                                 <  1:239953/148‑1 (MQ=255)                                                                                       aaaTAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAg                                                 <  1:123040/148‑1 (MQ=255)                                                                                       aaaTAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTCATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAg                                                 <  2:533174/148‑1 (MQ=255)                                                                                          tAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAat                                              <  1:379417/148‑1 (MQ=255)                                                                                          tAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTACGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAat                                              <  1:194849/148‑1 (MQ=255)                                                                                          tAAAACAGCAAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAat                                              <  2:425854/148‑1 (MQ=255)                                                                                           aaaaCAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAatt                                             <  2:356295/148‑1 (MQ=255)                                                                                              aCAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAattatt                                          <  1:850074/148‑1 (MQ=255)                                                                                               cAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAAttatta                                         >  1:340988/1‑148 (MQ=255)                                                                                                    aaaGTTATAAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAataa                                    >  2:94882/1‑147 (MQ=255)                                                                                                         tataAAAAGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCACAAATATAGAATTATTAAATATAatga                               >  1:927348/1‑148 (MQ=255)                                                                                                           aaaaaaGTGAAATAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAatgatg                             <  2:838126/147‑1 (MQ=255)                                                                                                            aaaaaGTGAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAACCACCAAAGATAGAATTATTAAATATAAtgatga                            <  2:956376/148‑1 (MQ=255)                                                                                                                   gAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTa                     >  2:702192/1‑148 (MQ=255)                                                                                                                   gAATTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTa                     >  2:566328/1‑148 (MQ=255)                                                                                                                    aaTTAATAACTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTaa                    >  1:48922/1‑148 (MQ=255)                                                                                                                      ttAATAATTAATTATATTATATTAGCCCTGCTTGAAAGAAAAAATAATTAGAATTAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATg                  <  1:377703/148‑1 (MQ=21)                                                                                                                         ataaCTAATTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATc               <  2:505181/148‑1 (MQ=255)                                                                                                                            acgaactatattatACTAGACACGTGTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGat            <  1:851433/142‑1 (MQ=25)                                                                                                                            aCTAATTATATAATATTAGCCACGCATCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATAATAAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGat            <  2:361354/148‑1 (MQ=37)                                                                                                                               aaTTATATTATATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGAtata         <  2:256062/148‑1 (MQ=255)                                                                                                                                 ttatattataTTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATGGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTAAATGATCGATataat       >  2:455486/1‑148 (MQ=255)                                                                                                                                     attatATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGc   <  2:151538/148‑1 (MQ=255)                                                                                                                                      ttatATTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCa  >  2:119739/1‑148 (MQ=255)                                                                                                                                          |                                                                                                                                                TTGATTAACTTAGGTAGTATCGGATACTTAAATGTTGGTTCATAAAAAGCAATGATTTTAAATCGAGGAGCTATCTTAGAACAGGGAAATAAAACAGCCAAAGTTATAAAAAGTGAATTAATAACTAATTATATTATGTTAGCCACGCTTCAAATAAAAAATAATTAGAATAAGGTGGGATTGATAATCAATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAATGATGAGTACTTAATGATCGATATAATAAGCA  >  CP000730/657395‑657675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑