| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 39,378 | C→T | 100% | D586N (GAT→AAT) | mecA ← | penicillin binding protein 2 prime |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 39,378 | 0 | C | T | 74.1% | 47.0 / 16.5 | 31 | D586N (GAT→AAT) | mecA | penicillin binding protein 2 prime |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/13); minor base A (7/1); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCATT > CP000730/39241‑39513 | aaTAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAttttc > 2:9092/1‑141 (MQ=255) aTAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAATCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAttttct > 2:258842/1‑141 (MQ=255) catGTTTGGATTATCTTTATCATATGATATAAACCACAAAATTTGTCTGCCAGGTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGtttt > 2:210599/1‑141 (MQ=255) tGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCGTAAGATCTATAAATATTTTCTTTATGTGTtttat > 2:167520/1‑141 (MQ=255) tGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTtttat > 2:55175/1‑141 (MQ=255) gTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTtttatt < 2:236066/141‑1 (MQ=255) tttGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTtttattt < 1:160421/141‑1 (MQ=255) aTCATATGATATAAATCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCAt < 1:258842/141‑1 (MQ=255) tGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATc > 1:209402/1‑141 (MQ=255) caccCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGa > 2:139817/1‑141 (MQ=255) ttCTCCTTGTTTCGTTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaat < 2:182785/141‑1 (MQ=255) ttCTCCTTGTTTCATTTTGAGTTCTGCACTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaat > 1:98684/1‑141 (MQ=255) ctcCATGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaataa > 1:191271/1‑141 (MQ=255) cATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTttctt < 2:236687/141‑1 (MQ=255) aGTTCTTCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATGTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACt < 1:201240/141‑1 (MQ=255) aGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACt < 1:9092/141‑1 (MQ=255) cAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGtttt > 1:117736/1‑141 (MQ=255) aGTATCGGATTTTCTAATTAAGTTTGTATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGttttt < 2:243286/141‑1 (MQ=255) cGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGGTTTATTAACGACTTGTTGCATACCATCAGTTAATAGCTTGATATTTTGTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCgcgt > 2:121976/1‑141 (MQ=255) gATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATCATATTTTTCTACCAAACTATGtgtaccgattct > 2:122682/1‑130 (MQ=255) gATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAAAAATATTTTTCTTCCAAACTTTGTTTTTTGTGTCt > 1:160396/1‑141 (MQ=255) aTTTGCCAATTAAGTTTGCATAAGAGCTATAAATATATTCTTTAGGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCtt > 1:153601/1‑141 (MQ=255) tttGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGATAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTAGTTTCttt > 2:13083/1‑141 (MQ=255) ttGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCtttt < 1:96872/141‑1 (MQ=255) ccAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTAAGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTAGTTTTTCGGGTCTTTtaat > 1:78180/1‑141 (MQ=255) tttGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGt > 1:275644/1‑141 (MQ=255) gCATAAGATCTATAAATATTTTCTTTATTTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCg < 2:102171/141‑1 (MQ=255) aTAAGCTCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGtt < 2:176135/141‑1 (MQ=255) aTAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGtt < 2:176121/141‑1 (MQ=255) aTAAAATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGtt < 2:265136/141‑1 (MQ=255) aagtttaataAATTTTTTCCTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTaa < 2:78934/134‑1 (MQ=255) aTCTATAAATATTTTCTTTATGTGTGTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTACAAATCGACTTTTTTCGTTGCTTTTAATAAGTGAGGTTCGGTAatat > 2:268257/1‑141 (MQ=255) aaTATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCatt < 1:67747/141‑1 (MQ=255) | AATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCATT > CP000730/39241‑39513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |