Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 646,838 G→A 55.0% intergenic (+192/‑395) lplA2 → / → mvaK1 lipoate‑‑protein ligase A/mevalonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730646,8380GA55.0% ‑2.3 / 22.5 20intergenic (+192/‑395)lplA2/mvaK1lipoate‑‑protein ligase A/mevalonate kinase
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base A (5/6);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

TCCATAAAAAGCATAAATATGTTATTGACCTAATATTTAAATTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTTAGTAGTAAATCTATATATTCCAATTATTATTTGTAGTATTATAGTTAGGGTGGAAAATTATAAATTGTAGGGCT  >  CP000730/646704‑646974
                                                                                                                                      |                                                                                                                                        
tCCATAAAAAGCATAAATATGTTATTGACCTAATATTTAAACTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAGTAAGGtata                                                                                                                                    >  1:191703/1‑141 (MQ=255)
     aaaaaGCAAAAATATGTTATTGACCTAATATTTAAATTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATaa                                                                                                                               <  1:121776/141‑1 (MQ=255)
      aaaaGCATAAATATGTTATTGACCTAATATTTAAATTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATAAt                                                                                                                              <  1:274770/141‑1 (MQ=255)
      aaaaGCATAAATATGTTATTGACCTAATATTTAAATTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATAAt                                                                                                                              >  2:274770/1‑141 (MQ=255)
           cATAAATATGTTATTGACCTAATATTTAAATTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAAAATCTGAGATGACGGATTAAATAGATTGAGAATAAGGTATATATAATGCGTa                                                                                                                         >  1:63570/1‑141 (MQ=255)
                              taataaTTAAATTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATAATGCGTATGACTATGAAAGAAACGAc                                                                                                      <  2:249759/141‑1 (MQ=255)
                                     tAAATTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTAt                                                                                               <  1:252907/141‑1 (MQ=255)
                                       aaTTGAACAACATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTg                                                                                             <  2:26911/141‑1 (MQ=255)
                                               atcatTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTc                                                                                     >  1:141084/1‑141 (MQ=255)
                                                   ttaaattaaaAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAAt                                                                                 <  2:186518/141‑1 (MQ=255)
                                                          aaaaGTAATGATATGATGGACATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAAAGTTCTAATACTTtag                                                                          <  1:207269/141‑1 (MQ=255)
                                                             aGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTtagtag                                                                       <  2:31383/141‑1 (MQ=255)
                                                               tAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTTAgtagta                                                                     <  2:161169/141‑1 (MQ=255)
                                                               tAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTTAgtagta                                                                     <  2:133497/141‑1 (MQ=255)
                                                                             aaaTAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTTAGTAGTAAATCTATATATTcc                                                       >  1:190249/1‑141 (MQ=255)
                                                                                                              tACGTATTAAATAGATTGAGAATAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTTAGTAGTAAATCTATATATTCCAATTATTATTTGTAGTATTATAGTTAGGGTGGa                      >  2:227241/1‑141 (MQ=255)
                                                                                                                    ttAAATAGATTTAGAATAGGGTATATATAATGCGTATGACTATGAAAAAACCGACCTTCTATTGTAATGTTCTAATACTTTATTAGTATATCTATATAGGCCAATTATTATTTGTAGTAATAGAGTTAGGGTGGAAAATta                <  2:219505/141‑1 (MQ=255)
                                                                                                                    ttAAATAGAGTGAGAATAGGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGCAATGTTCTAATACTTTAGTAGTAAATCTATATATTCCAATTATTATTTGTAGTATTATAGTTAGGGTGGAAAATta                >  2:276164/1‑141 (MQ=255)
                                                                                                                             ttGAGAATAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTTAGTAGTAAATCTATATATTCCAATTATTATTTGTAGTATTATCGTTAGGGTGGAAAATTATCAATTGTa       >  1:182206/1‑141 (MQ=255)
                                                                                                                                  aaTAAGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTTAGTAGTAAATCTATATATTCCAATTATTATTTGTAGTATTATAGTTAGGGTGGAAAATTATAAATGGTAGGGCt  >  1:11160/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TCCATAAAAAGCATAAATATGTTATTGACCTAATATTTAAATTGAACATCATTAAATTAAAAGTAATGATATGATGGAAATAATAAATTTAATAAGAAAATTTCATGTTTTACGTATTAAATAGATTGAGAATAGGGTATATATAATGCGTATGACTATGAAAGAAACGACCTTCTATTGAAATGTTCTAATACTTTAGTAGTAAATCTATATATTCCAATTATTATTTGTAGTATTATAGTTAGGGTGGAAAATTATAAATTGTAGGGCT  >  CP000730/646704‑646974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: