| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,296,417 | C→T | 13.4% | A137V (GCG→GTG) | pnp → | polyribonucleotide nucleotidyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,296,417 | 0 | C | T | 13.4% | 69.1 / 4.7 | 30 | A137V (GCG→GTG) | pnp | polyribonucleotide nucleotidyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (15/11); new base T (2/2); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01 | |||||||||||
TAATTGATAGACCAATTAGACCTTTATTCCCTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGAAGTAGCTGGTCATAA > CP000730/1296288‑1296555 | tAATTGATAGACCAATTAGACCTTTATTCCCTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAgtgtgt < 2:199814/141‑1 (MQ=255) tGATAGACCAATTAGACCTTTATTCCCTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGGGTGTCGGa > 2:64930/1‑141 (MQ=255) aCCAATTAGACCTTTATTCCCTAAAGGATATAAGCATGATGTTCACATTCTGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATattcc > 1:162595/1‑141 (MQ=255) cAATTGGACCTTTAATCCCTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATattccat < 2:23431/141‑1 (MQ=255) tttATTCCCTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTTGTTCATCTATGGCGATTGGTTTGTCGGATATTCCATTCCAAGGGcc < 1:135458/141‑1 (MQ=255) cccTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGTGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGc > 2:189256/1‑141 (MQ=255) cccTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGTGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGc > 2:189123/1‑141 (MQ=255) aaaGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGgt > 1:232367/1‑141 (MQ=255) gatgatgTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGCTTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCCATTTCCGGTGTAAATGTGGGt < 1:149085/140‑1 (MQ=255) cATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAACCGCCGGTGTAAATGTGGGt < 1:149092/141‑1 (MQ=255) cATGATGTTCAAATGATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGt < 1:234989/141‑1 (MQ=255) ttCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTg < 2:279681/141‑1 (MQ=255) tcATGGTTTTTCGTGCAGTTCCTGGTTGTCTCCCACAAATGGCTGCAATGATTGGTTCATCTATGTCGCTTTGTGTGTCGGATATTCCATTTCAAGGGCCAATCGCTTGTGTTAATGTGGGTTATTTTGACGGTAAatata < 2:71802/140‑1 (MQ=255) aGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCa > 1:41403/1‑141 (MQ=255) aTTGTTCACCACAAATGGCTGCAATTATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATCCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGaaa < 1:26725/141‑1 (MQ=255) tCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAaagag > 1:119243/1‑140 (MQ=255) caccacAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAaagaag > 1:155991/1‑141 (MQ=255) aTGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAATGTCGCGt > 1:188298/1‑141 (MQ=255) ggCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGttt > 1:169542/1‑141 (MQ=255) tGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTg > 2:232710/1‑141 (MQ=255) gATTGGTTCATCTATGGTGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGa < 1:189123/141‑1 (MQ=255) gATTGGTTCATCTATGGTGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGa < 1:189256/141‑1 (MQ=255) gATTGGGTCATCTATGCCGCTGAGTGTGCCTGATATTCCATGCCAAGGGCCAATAGAAGGTCTGAACTTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGa < 1:64930/141‑1 (MQ=255) ttGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGAAg > 2:177473/1‑141 (MQ=255) tGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCGAGGTCCAATCGCCGGTGTAAATGTGGGTTTTATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAGAGAAGGTTCTCGTTTAAACCTAGATGt > 1:149823/1‑141 (MQ=255) gTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGAAGTAg > 2:248127/1‑141 (MQ=255) tATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTATAAATGTGGGTTATATTGACGGTAAATATGTCATTAACCCAACAGTGGAAGAAAAAAAAGTTTCTCGTTTCAACCTGAAAGTAGATGGTCa > 2:75961/1‑141 (MQ=255) tGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGAAGTAGCTGGTCATa > 1:237072/1‑141 (MQ=255) gggCGCTTAGGGAGTCGGAGATTCCATGACAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAAAAAAAAAAAAAAAAGAAGTTTCTCGTTTAGACCTTGAAGTAGCTGGTCATa < 2:41917/140‑1 (MQ=255) ggCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGATTAGACCTTGAAGTAGCTGGtaataa > 2:47234/1‑141 (MQ=255) | TAATTGATAGACCAATTAGACCTTTATTCCCTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCAGATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTGTCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAATATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGAAGTAGCTGGTCATAA > CP000730/1296288‑1296555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |