Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,481,355 G→T 100% A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,481,3550GT100.0% 80.3 / NA 27A6890E (GCA→GAA) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/12);  total (15/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGC  >  CP000730/1481222‑1481488
                                                                                                                                     |                                                                                                                                     
cGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGtt                                                                                                                                >  1:232719/1‑141 (MQ=255)
       ttCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTGGCa                                                                                                                         >  1:246956/1‑141 (MQ=255)
            ttCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGt                                                                                                                    <  2:61255/141‑1 (MQ=255)
                     ggTTTGGACGGCGTGCGTTACTTCAGCTACTGTGTTCGCTTGATTTATGTTTCCATTAACAGTACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCt                                                                                                           <  2:252799/141‑1 (MQ=255)
                                cTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTGTTTAGCATCTGTTAATTTTCAATCACCATTCa                                                                                                >  1:28774/1‑141 (MQ=255)
                                                 aCAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgtt                                                                               >  1:212476/1‑141 (MQ=255)
                                                    gtgtGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttgtt                                                                            >  1:230132/1‑141 (MQ=255)
                                                        gcgcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCAACATTCAACGCTTGTTTCGTAGTTGTTAAAg                                                                        >  2:177631/1‑141 (MQ=255)
                                                        gcgcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGAATCCGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAg                                                                        >  2:167691/1‑141 (MQ=255)
                                                         cgcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGt                                                                       <  2:83437/141‑1 (MQ=255)
                                                           cTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTg                                                                     >  1:46777/1‑141 (MQ=255)
                                                                  gatGTTTCCAGTAACAGCACATTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTATTCATTGTTTTTAGTATCTGTTACTTTTATATCACCATTCAAAGCTTGTTTGGTAGTTGTTAAAGTGTAACGtgc                                                              >  2:211408/1‑141 (MQ=255)
                                                                        tCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTc                                                        >  1:74263/1‑141 (MQ=255)
                                                                              aaCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtg                                                  <  1:54985/141‑1 (MQ=255)
                                                                               actgCACCTTTTTGTGCATTGTTTAAGTTATCGAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgt                                                 <  1:86223/138‑1 (MQ=255)
                                                                               aCAGCTCTTTTGTTTGCATTGTTTAGGTTATCTAACGTAGATAATGTTTGGGTTTCATTGTTTTTAGCATCTGTTTCTTTTCTATCACCATTCATCTCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgt                                                 <  1:61158/141‑1 (MQ=255)
                                                                               aCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTCCAGTTGAAAGTGCTGCTTCACCTtgt                                                 >  2:63225/1‑141 (MQ=255)
                                                                                    aCCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCCTCAACTTGTGATTg                                            >  2:97816/1‑141 (MQ=255)
                                                                                           tgtgCATTGTTTAAGGTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCa                                     <  1:118249/141‑1 (MQ=255)
                                                                                              gCATTGTTGAAGTTATCTGACGGAGATAATGTTTTGTTTTCATTGTTTTTGGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCAttt                                  <  2:142376/141‑1 (MQ=255)
                                                                                                attggctAAGTTATCTAACGGAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTAGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGt                                <  1:168817/135‑1 (MQ=255)
                                                                                                  tGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTAc                              <  2:246956/141‑1 (MQ=255)
                                                                                                    tttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCa                            <  2:230132/141‑1 (MQ=255)
                                                                                                          ttATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCt                      <  1:101616/141‑1 (MQ=255)
                                                                                                             tCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAAGGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCCTTTGTAGCATTTAGTTGa                   >  2:84460/1‑141 (MQ=255)
                                                                                                                 aCGTAGATGATGAAAGGTTTTCATTGATTTTAGCATCTGTTACCATTCTATCCCCATACAACGCGTGTTTCGTAGTTGTTACAGTCGAAAGTGCTGCTTCAACTTGTGGTCGGTTCGCATTTGTTCCATTGGCTAAATtaa               >  2:199446/1‑141 (MQ=255)
                                                                                                                   gTAGATAATGTTTGGTTTTCATTGTCTTTAGCATCTGTTACTTTGCTATCACCATTCAACGACTGTTTCGTAGTTGTTACAGTTGAAAGTTCTGCGTCCACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGacaaaat             >  2:69150/1‑135 (MQ=255)
                                                                                                                              ttggttttCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcagc  <  2:236473/141‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGC  >  CP000730/1481222‑1481488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑