| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,834,119 | G→T | 67.9% | intergenic (+68/‑46) | ymaD → / → sgaA | hypothetical protein/class V aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,834,119 | 0 | G | T | 67.9% | 17.1 / 19.5 | 25 | intergenic (+68/‑46) | ymaD/sgaA | hypothetical protein/class V aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (4/4); new base T (10/7); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01 | |||||||||||
ATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCAAGCACCTATGGTTG > CP000730/1833989‑1834252 | aTTTCAAAGCCTGTAAGAAATAATGGGTCAATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCTACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCt < 2:126341/141‑1 (MQ=255) cTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTAGAGCAGATAGCATTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGa > 1:64747/1‑141 (MQ=255) aGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCt > 1:117871/1‑141 (MQ=255) aTAAAACTCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCa < 1:237582/141‑1 (MQ=255) aTAAAAATCTATCCCATCATTCAGGCCAAATAAAGTCGTAAAGTGAAGACGTTTGTTAGACAATAAAGCGTTATCACTTTTTTAGGCATTTAAATTTTTATAAAATAGGATGAAATAGTTAAATATTATTTCTTGGAGACa > 2:87068/1‑141 (MQ=255) aaaTCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCAtata < 2:250654/141‑1 (MQ=255) ccccATCCTTCAGGCCAAATAAAATCGTTAAGGCATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATCATCATAAAATTTGTTTGCTTGGGGAAATCTTATTTATAGGAGGCATATAAATGtat < 1:134478/140‑1 (MQ=255) ccATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATATGAGGCATATAAATGtatta > 2:197960/1‑141 (MQ=255) catTCAGGGCCAATAAAATTGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTatcat < 2:121351/141‑1 (MQ=255) catTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTatcat < 1:279914/141‑1 (MQ=255) aGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAAcc > 1:224109/1‑141 (MQ=255) cGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAacac > 1:267053/1‑141 (MQ=255) cGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAacac > 1:55558/1‑141 (MQ=255) tCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAGATCTTATTTATAGGAGGCATATAAGTGTATTATCATCAACGGGTGT‑TATTAACACCTGGCCCAACCCCTGGACCt > 1:17345/1‑141 (MQ=255) tCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGCGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCt > 1:17332/1‑141 (MQ=255) tCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCt > 2:219877/1‑141 (MQ=255) aCAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGAt < 1:244459/141‑1 (MQ=255) tATCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATgaga < 2:64747/141‑1 (MQ=255) tCACTTTTTTAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTCCCTGATGCCATTATgagaga < 2:116162/141‑1 (MQ=255) ttttttAGGCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGATGACATTATGAGAGAAAtt > 1:174518/1‑141 (MQ=255) tttttAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTc > 1:18239/1‑141 (MQ=255) tttttAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTc > 1:10436/1‑141 (MQ=255) ttttAGGCTTCTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCAT‑AACCTGCGTGTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCa > 1:203379/1‑141 (MQ=255) gCTTTTAAATTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCAAGCAcc < 2:13313/141‑1 (MQ=255) aTTTTCATAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCAAGCACCTAtggttg < 2:267053/141‑1 (MQ=255) | ATTTCAAATGCTGTAAGAAATAATGTGGACATAAAAATCTATCCCATCATTCAGGCCAAATAAAATCGTTAAGTGATAACGTTTGTTCGACAATATAGCGTTATCACTTTTTTAGGCTTTTAAATTTTCAGAAAATTTGCTAAAATAGTGAAATCTTATTTATAGGAGGCATATAAATGTATTATCATCAACCGTTGT‑TATTAACACCTGGCCCAACCCCTGTACCTGATGCCATTATGAGAGAAATTCAAGCACCTATGGTTG > CP000730/1833989‑1834252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |