Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT)  murN → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 82.3 / NA 27T15T (ACA→ACT) murNFemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/16);  total (11/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAG  >  CP000730/2532163‑2532431
                                                                                                                                       |                                                                                                                                     
tgtATTTGTAATCAGTTTTGTATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                  <  2:71088/141‑1 (MQ=255)
   aTTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCa                                                                                                                               <  1:171797/141‑1 (MQ=255)
              gTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAtt                                                                                                                    >  2:104775/1‑141 (MQ=255)
                 tttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTaca                                                                                                                 <  1:119458/141‑1 (MQ=255)
                        aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTGAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGTATGAGTTCAATGCGTTTACTACAAAGCATTTTTCAAATTACACACAATc                                                                                                          >  2:73205/1‑141 (MQ=255)
                                  aTAGCCTTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAACAGTGTTCACATTACACACAATCAGCTATTCAt                                                                                                <  1:10713/141‑1 (MQ=255)
                                  aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTGTTCACATTACACACAATCAGCTATTCAt                                                                                                <  1:10714/141‑1 (MQ=255)
                                    aGCATTATTAATAAATACAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAttc                                                                                              >  1:139403/1‑140 (MQ=255)
                                            taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                      <  1:271080/141‑1 (MQ=255)
                                               taaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATagag                                                                                   >  2:117737/1‑141 (MQ=255)
                                                   ttCAAAAATTATAGTTCAATTCAGAGATGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGa                                                                               <  1:5519/141‑1 (MQ=255)
                                                                  tCAAGTCAGAGAGGGATGGGATAAAATGATCTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAACCATAGAGTTGATTTAAAAGGCGAtgt                                                                <  1:159007/141‑1 (MQ=255)
                                                                   tatttCAGACAGCGATGGAATAAATTCAACTTTGTAATGTTGACTTTGGTTGAGTTCAATGCGTTTACTACAAAGCATTTGTCACATTACACACAATCCGCTATTCATTACAATCATAGAGTTTATTTAAAAGGCGAtgtg                                                               <  1:80399/138‑1 (MQ=255)
                                                                          gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTCGATCTAAAACGCGACGTGCATCttc                                                        >  2:158625/1‑140 (MQ=255)
                                                                               ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg                                                   <  2:152395/141‑1 (MQ=255)
                                                                                  tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTGACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAGTTAAAAGGCGATGTGCATCTTGTAGGGGta                                                >  2:205987/1‑140 (MQ=255)
                                                                                   gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTa                                               <  2:246180/141‑1 (MQ=255)
                                                                                      atAAAATGAACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg                                            <  1:44993/141‑1 (MQ=255)
                                                                                      acaaaaTGAAATTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg                                            <  1:96632/139‑1 (MQ=255)
                                                                                                      aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGt                            <  2:18833/141‑1 (MQ=255)
                                                                                                            gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGGAGGGGTTAAAGATGACAATGGTCAAGTGATTGc                      >  1:29457/1‑141 (MQ=255)
                                                                                                              cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCACGTGCTTGCAg                    >  2:114699/1‑141 (MQ=255)
                                                                                                               ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                   <  1:246426/141‑1 (MQ=255)
                                                                                                                     tGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCtt             >  1:104178/1‑141 (MQ=255)
                                                                                                                        gTTCAATGCGTTTAGTACAAAGCATTTTTAACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt          >  1:149748/1‑141 (MQ=255)
                                                                                                                            aaTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACa      >  1:27888/1‑141 (MQ=255)
                                                                                                                                cGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg  <  1:117737/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAG  >  CP000730/2532163‑2532431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑