| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,679,708 | G→A | 64.6% | W64* (TGG→TGA) | cbpD → | possible secretory antigen |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,679,708 | 0 | G | A | 64.6% | 15.6 / 11.8 | 22 | W64* (TGG→TGA) | cbpD | possible secretory antigen |
| Reads supporting (aligned to +/- strand): ref base G (3/5); new base A (8/6); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTT > CP000730/2679584‑2679825 | cTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAAtt < 2:102308/141‑1 (MQ=255) aaTCCGTCGAATAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTAtt < 2:79970/141‑1 (MQ=255) cGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGATATTACAATGc > 1:74708/1‑141 (MQ=255) gAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACaaggga > 1:73608/1‑137 (MQ=255) gCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATc > 1:167682/1‑141 (MQ=255) attcaTCGCATGCCGCAGTTAATTGTTAGAGTAAAGACCAAATCACATGGTGTGTATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCaa < 1:106245/137‑1 (MQ=255) aTCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGGTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAAtacaagta > 1:50197/1‑134 (MQ=255) gCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGc < 2:47635/141‑1 (MQ=255) aTGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTTGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGGATTACAATGCAGGTAAATGAAAATATgaga > 2:105779/1‑141 (MQ=255) tattatAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAGAAATTGGTATTACAATGCACGTAACTAAAAATATGGCACTGGCCGTACAC‑Ca > 2:38650/1‑141 (MQ=255) ccAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGGCGGTAAACCTGTTGCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGt < 2:4522/141‑1 (MQ=255) ccAATGTACAGGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATAGGGCAATGCTAAAAATTAGTCGTACAATGCACGTAAATCAAAATATGAGACTGGTAGT‑CACACAAGAAACGTTGcagg > 2:37007/1‑138 (MQ=255) aTGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCGGTCAt > 2:145611/1‑141 (MQ=255) cATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAAt < 1:165430/141‑1 (MQ=255) cATGGTGGGCAATTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAAt < 2:158892/141‑1 (MQ=255) aaTTTCAACGTGGCGCAAAAGTCGGTCACTCTTTTGCGAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGGAAATCAAAAGATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAg < 1:244051/140‑1 (MQ=255) tAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAGGata > 2:177111/1‑141 (MQ=255) aCGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAGGAtattt > 1:94667/1‑140 (MQ=255) caAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTc < 1:197942/141‑1 (MQ=255) caAGTCGGTAAACCTGTTTCTAAGAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTc < 2:229046/141‑1 (MQ=255) caAGGCGGTAAACCGGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTc < 2:229054/141‑1 (MQ=255) ggTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTAGccgat > 1:130964/1‑137 (MQ=255) | CTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACAC‑CAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTT > CP000730/2679584‑2679825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |