Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 479,553 G→A 100% A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730479,5530GA100.0% 87.5 / NA 29A194T (GCA→ACA) USA300HOU_0458hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTAC  >  CP000730/479418‑479687
                                                                                                                                       |                                                                                                                                      
gATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATgg                                                                                                                                   <  2:180902/141‑1 (MQ=255)
   gCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTg                                                                                                                                <  1:16412/141‑1 (MQ=255)
           aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgc                                                                                                                        <  1:51257/141‑1 (MQ=255)
           aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgc                                                                                                                        <  1:200316/141‑1 (MQ=255)
           aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAAGCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgc                                                                                                                        <  1:47783/141‑1 (MQ=255)
             aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATTAGTACAAGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgcgc                                                                                                                      <  2:127229/141‑1 (MQ=255)
              tGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACGATGCCGAAGCGTGTGCGAAGTTTTTACCATACATGGTTGCATTTAGCGCa                                                                                                                     >  2:84044/1‑141 (MQ=255)
                        gAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTGTATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCAt                                                                                                           <  1:270133/141‑1 (MQ=255)
                          gCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTATCGCAACATGACCATAg                                                                                                         >  2:163387/1‑141 (MQ=255)
                               ttAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTa                                                                                                    >  2:251906/1‑141 (MQ=255)
                                tAGCGAACAAGTGAAGCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTAc                                                                                                   <  1:130427/141‑1 (MQ=255)
                                                cGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCaa                                                                                   <  1:103001/141‑1 (MQ=255)
                                                 gTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAg                                                                                  <  1:179671/141‑1 (MQ=255)
                                                           tAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAgg                                                                        >  2:207077/1‑141 (MQ=255)
                                                                 gACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGAGCCAAGTGATTAAAGGCTTAcc                                                                  >  1:92254/1‑141 (MQ=255)
                                                                           tataTCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACAATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAGTGATCCt                                                        >  2:58477/1‑141 (MQ=255)
                                                                             tatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGa                                                      >  1:8777/1‑141 (MQ=255)
                                                                              atCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCCACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGaa                                                     <  1:263280/141‑1 (MQ=255)
                                                                                        ttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATag                                           <  1:157132/141‑1 (MQ=255)
                                                                                        tcccaTCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACCTGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATag                                           <  1:18751/137‑1 (MQ=255)
                                                                                                 gTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTAt                                  <  2:92142/141‑1 (MQ=255)
                                                                                                    ggACAATGCCGAAGCGTGCGAATAGTACATACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATCGAGGCAGTCTTaa                               >  1:135820/1‑141 (MQ=255)
                                                                                                              gAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTGACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATCACGATAGAGTCAGAATTAACTCAttttta                     >  2:27256/1‑140 (MQ=255)
                                                                                                                 gCGTGAGCAAAGTTTTTACCACACATGGTTCCATATAGAGCAACAGCACCATCGTTCTACTAAAGCCCAGCGCCAAGTCATAGAACACTCACCCCATGATCCTGAAATGCCACTACAGTCAGAATCACCTCAtttttaaca                  >  2:184751/1‑137 (MQ=255)
                                                                                                                      aGCAAAGTTTTTACAATACATGGTTGCATCTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATc             >  1:149808/1‑141 (MQ=255)
                                                                                                                             tttttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGa      >  2:253088/1‑141 (MQ=255)
                                                                                                                                ttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAATTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTa   <  1:214054/141‑1 (MQ=255)
                                                                                                                                 tACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTAc  >  1:27533/1‑141 (MQ=255)
                                                                                                                                 tACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTAc  <  1:251906/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTAC  >  CP000730/479418‑479687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: