Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 942,163 (A)6→5 intergenic (‑134/+115) USA300HOU_0905 ← / ← mnhG2 hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730942,1580A.100.0% 151.6 / NA 31intergenic (‑129/+120)USA300HOU_0905/mnhG2hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/11);  total (20/11)

TGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATCGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCT  >  CP000730/942018‑942293
                                                                                                                                            |                                                                                                                                       
tGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGaaaaatca                                                                                                                                  >  2:311369/1‑145 (MQ=255)
 gTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGaaaaatcat                                                                                                                                 >  2:45040/1‑144 (MQ=255)
        gCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGaaaaa                                                                                                                         <  1:263446/148‑1 (MQ=255)
          ataataAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAaga                                                                                                                       <  2:101365/148‑1 (MQ=255)
            aataaAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAgg                                                                                                                     <  2:140806/148‑1 (MQ=255)
                 aaCCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACa                                                                                                                >  2:75522/1‑148 (MQ=255)
                 aaCCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACa                                                                                                                >  2:75345/1‑148 (MQ=255)
                 aaCCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACa                                                                                                                >  1:67090/1‑148 (MQ=255)
                    ctctctctTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATg                                                                                                             <  2:280012/148‑1 (MQ=255)
                    ctctctctTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATg                                                                                                             <  2:58891/148‑1 (MQ=255)
                                 aaaCGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAAca                                                                                                >  2:48058/1‑148 (MQ=255)
                                 aaaCGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAAca                                                                                                >  2:273308/1‑148 (MQ=255)
                                                      taATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAAtt                                                                           <  1:82185/148‑1 (MQ=255)
                                                      taATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAAtt                                                                           <  1:114397/148‑1 (MQ=255)
                                                           attattatCATAATACATAACGACATGAAAGGCAATTACATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTACTAATTTGGaa                                                                      >  2:325540/1‑148 (MQ=255)
                                                           attattatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGaa                                                                      >  2:16010/1‑148 (MQ=255)
                                                               ttatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatcat                                                                  <  1:92698/148‑1 (MQ=255)
                                                                   cATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa                                                              >  2:231424/1‑148 (MQ=255)
                                                                                 aCATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgt                                                <  1:23422/148‑1 (MQ=255)
                                                                                 aCATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgt                                                >  2:125363/1‑148 (MQ=255)
                                                                                 aCATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgt                                                >  2:125330/1‑148 (MQ=255)
                                                                                                    taaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAttt                             <  1:166862/148‑1 (MQ=255)
                                                                                                       agagATATATGTAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGt                          <  2:370238/148‑1 (MQ=255)
                                                                                                                  tAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa               >  2:27878/1‑148 (MQ=255)
                                                                                                                  tAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATAATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa               >  2:168244/1‑148 (MQ=255)
                                                                                                                  tAGATAGGGCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAAGGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATAATCATGAGTTGGTTATATAATCTa               >  2:324139/1‑148 (MQ=255)
                                                                                                                        gggCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAttt         >  2:184900/1‑148 (MQ=255)
                                                                                                                        gggCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAttt         >  1:184734/1‑148 (MQ=255)
                                                                                                                         ggCGAATCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAtttt        >  1:120576/1‑148 (MQ=255)
                                                                                                                               tCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCt  >  1:184959/1‑148 (MQ=255)
                                                                                                                               tCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCt  >  1:165167/1‑148 (MQ=255)
                                                                                                                               tCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCt  >  1:355703/1‑148 (MQ=255)
                                                                                                                               tCTGTAGTCAAAG‑AAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCt  >  1:81548/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                       
TGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATCGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCT  >  CP000730/942018‑942293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑