Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,719,269	(A)5→6	coding (213/705 nt)	USA300HOU_2573 →	dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,719,264	1	.	A	100.0%	158.0 / NA	42	E70K (GAA→AAA)	USA300HOU_2573	dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (18/24);  total (18/24)

AAAGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACAT  >  CP000730/2719123‑2719405                                                                                                                                               |                                                                                                                                              aaaGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaa                                                                                                                                         >  1:78562/1‑147 (MQ=255)   aGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaaga                                                                                                                                       <  2:25506/148‑4 (MQ=255)     cATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaagaag                                                                                                                                     >  1:76319/1‑143 (MQ=255)     cATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaagaag                                                                                                                                     >  1:283666/1‑143 (MQ=255)     cATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑aaaaaagaag                                                                                                                                     >  1:268408/1‑143 (MQ=255)           gTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTc                                                                                                                                >  2:312899/1‑148 (MQ=255)            ttACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCt                                                                                                                               <  2:206121/148‑1 (MQ=255)            ttACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCt                                                                                                                               <  1:214801/148‑1 (MQ=255)            ttACAAAGTTTATATAGGTTATCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATAATTGCAGAAAAAAGAAGGCCGTCt                                                                                                                               <  2:171222/148‑1 (MQ=255)            ttACAAAGTTAAAATAGGCTTTCGAAATGATGTAAGAGGACAAGAAGCATCACAAAGATTAGGTGTTCAGTATGTACTATTAGATGTTACAAATGAATACAAAAGCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCt                                                                                                                               <  2:294370/148‑1 (MQ=255)                     ttATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCt                                                                                                                      >  1:43166/1‑148 (MQ=255)                     ttATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCt                                                                                                                      <  2:118069/148‑1 (MQ=255)                                 tcGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGc                                                                                                          <  2:251313/148‑1 (MQ=255)                                 tcGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGc                                                                                                          <  1:79959/148‑1 (MQ=255)                                   ggAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAg                                                                                                        <  1:18815/148‑1 (MQ=255)                                      atgatgTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGta                                                                                                     >  2:203836/1‑148 (MQ=255)                                           gTAAGAGGACAACAAACATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCt                                                                                                <  1:155551/148‑1 (MQ=255)                                             aaGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTgg                                                                                              <  2:137328/148‑1 (MQ=255)                                                agGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCa                                                                                           >  2:309951/1‑148 (MQ=255)                                                    caacaaGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAAttt                                                                                       >  1:96522/1‑148 (MQ=255)                                                      acaaGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTc                                                                                     <  1:26466/148‑1 (MQ=255)                                                       caaGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCa                                                                                    >  2:350547/1‑148 (MQ=255)                                                              cacaAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCtt                                                                             >  1:341335/1‑148 (MQ=255)                                                              cacaAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCtt                                                                             >  1:14675/1‑148 (MQ=255)                                                                  aaaaTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATCTCTGGTCAATTTTCAGCACCTTCCaa                                                                         >  2:303155/1‑148 (MQ=255)                                                                  aaaaTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCaa                                                                         >  2:291943/1‑148 (MQ=255)                                                                  aaaaTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCaa                                                                         >  1:98539/1‑148 (MQ=255)                                                                     aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      <  2:94083/148‑1 (MQ=255)                                                                     aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      <  1:133019/148‑1 (MQ=255)                                                                     aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      <  1:113753/148‑1 (MQ=255)                                                                     aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      <  2:358092/148‑1 (MQ=255)                                                                     aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      >  2:280740/1‑148 (MQ=255)                                                                     aTTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAAtt                                                                      >  2:250687/1‑148 (MQ=255)                                                                          gtgtTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGacac                                                                 >  2:300536/1‑148 (MQ=255)                                                                                 ttATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGa                                                          <  1:203836/148‑1 (MQ=255)                                                                                  tATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAACAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGAc                                                         >  2:196646/1‑148 (MQ=255)                                                                                        cAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaa                                                   >  1:93313/1‑148 (MQ=255)                                                                                        cAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCAGGAAAATTGTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaa                                                   >  1:291891/1‑148 (MQ=255)                                                                                         aattcaaTGTTAAAAGTGACTACTCAGTAAACAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTCACAATGCAGGTATATCTGGTCAATTTACAGCACCTTCCAAATTGACACCTCGTGACGTTgaag                                                  <  2:229018/142‑1 (MQ=255)                                                                                           ttAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaggaa                                                <  2:259408/148‑1 (MQ=255)                                                                                           ttAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTgaagaa                                                <  1:128066/148‑1 (MQ=255)                                                                                             aGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGt                                              >  2:135154/1‑148 (MQ=255)                                                                                                   tACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCa                                        <  1:308293/148‑1 (MQ=255)                                                                                                   tACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCa                                        <  2:106897/148‑1 (MQ=255)                                                                                                                           tGCTTATAACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACg                <  1:309951/148‑1 (MQ=255)                                                                                                                               tataACATGATTGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAatg            <  1:350547/148‑1 (MQ=255)                                                                                                                                         ttGCTGAAAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACAt  <  2:78562/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              AAAGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAACAAGCATCACAAAAATTAGGTGTTCATTATGTACAATTAGATGTTACAAGTGACTACTCAGTCAAAAATGCTTATAACATGATTGCTG‑AAAAAGAAGGCCGTCTTGATATCCTCATTAACAATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAAGAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACAT  >  CP000730/2719123‑2719405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑