Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 942,060 C→G intergenic (‑31/+218) USA300HOU_0905 ← / ← mnhG2 hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730942,0600CG100.0% 132.9 / NA 35intergenic (‑31/+218)USA300HOU_0905/mnhG2hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (19/16);  total (19/16)

AAAAAGTTTCGTGTGGGTCAATGATTGTTAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATCGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACA  >  CP000730/941915‑942199
                                                                                                                                                 |                                                                                                                                           
aaaaaGTTTCGTGTGGGTCAATGATTGTTAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGt                                                                                                                                           <  1:102021/148‑1 (MQ=255)
  aaaGTTTCGTGTGGGTCAATGATTGTTAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTa                                                                                                                                         >  1:211652/1‑148 (MQ=255)
  aaaGTTTCGTGTGGGTCAATGATTGTTAATCGGTCTGTTGTTAAGCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTa                                                                                                                                         >  1:276596/1‑148 (MQ=255)
              gggTCAATGATTGTTAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAatt                                                                                                                             <  1:62532/148‑1 (MQ=255)
                        ttGTTAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCa                                                                                                                   >  1:58891/1‑148 (MQ=255)
                        ttGTTAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCa                                                                                                                   >  1:280012/1‑148 (MQ=255)
                           ttAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAGCCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCataa                                                                                                                <  1:176614/148‑1 (MQ=255)
                           ttAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCataa                                                                                                                <  1:88337/148‑1 (MQ=255)
                           ttAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCataa                                                                                                                <  2:241097/148‑1 (MQ=255)
                                cGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACAt                                                                                                           >  2:166862/1‑148 (MQ=255)
                                         gttAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATg                                                                                                  >  2:23422/1‑148 (MQ=255)
                                                cGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCa                                                                                           <  2:175914/148‑1 (MQ=255)
                                                cGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCa                                                                                           <  2:353229/148‑1 (MQ=255)
                                                cGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCa                                                                                           <  1:129229/148‑1 (MQ=255)
                                                 gCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCaa                                                                                          <  2:60275/148‑1 (MQ=255)
                                                     gATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCaattaa                                                                                      <  1:23424/148‑1 (MQ=255)
                                                       taataGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCaattaaat                                                                                    >  1:336998/1‑148 (MQ=255)
                                                        aataGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCaattaaatt                                                                                   <  2:51314/148‑1 (MQ=255)
                                                                     cGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATg                                                                      >  1:74333/1‑148 (MQ=255)
                                                                     cGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATg                                                                      >  2:346795/1‑148 (MQ=255)
                                                                     cGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATGTAATTAAAGAGATATATg                                                                      >  1:3188/1‑148 (MQ=255)
                                                                                       tGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGt                                                    >  1:370238/1‑148 (MQ=255)
                                                                                            ccgccgATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTATGATTTATAATTATTACTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCGGTAGTCa                                               >  2:11456/1‑148 (MQ=255)
                                                                                                       tGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGaaaaatca                                    >  2:311369/1‑145 (MQ=255)
                                                                                                        gTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGaaaaatcat                                   >  2:45040/1‑144 (MQ=255)
                                                                                                               gCATAATAAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGaaaaa                           <  1:263446/148‑1 (MQ=255)
                                                                                                                 ataataAAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAaga                         <  2:101365/148‑1 (MQ=255)
                                                                                                                   aataaAACCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAAGAgg                       <  2:140806/148‑1 (MQ=255)
                                                                                                                        aaCCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAAGAGGTAACa                  >  2:75345/1‑148 (MQ=255)
                                                                                                                        aaCCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAAGAGGTAACa                  >  1:67090/1‑148 (MQ=255)
                                                                                                                        aaCCTCTCTCTTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAAGAGGTAACa                  >  2:75522/1‑148 (MQ=255)
                                                                                                                           ctctctctTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAAGAGGTAACAATg               <  2:280012/148‑1 (MQ=255)
                                                                                                                           ctctctctTTTTAAAACGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAAGAGGTAACAATg               <  2:58891/148‑1 (MQ=255)
                                                                                                                                        aaaCGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAAca  >  2:273308/1‑148 (MQ=255)
                                                                                                                                        aaaCGTAATGGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAAca  >  2:48058/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
AAAAAGTTTCGTGTGGGTCAATGATTGTTAATCGGTCTGTTGTTAATCCGCTTGATAATAGTTTTTGTGCGATTGCAGTTCCCTGTATGCCACCGCCGATAATTGTCCAATGCATAATAAAACCTCTCTCTTTTTAAAACGTAATCGTTACGATTTATAATTATTATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACA  >  CP000730/941915‑942199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: