Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,420,434	G→A	A229T (GCG→ACG)	oppD →	M3 family oligoendopeptidase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,420,434	0	G	A	100.0%	128.2 / NA	35	A229T (GCG→ACG)	oppD	M3 family oligoendopeptidase F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/12);  total (23/12)

CGATTCATTTGAACATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCGCGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAACGCGTGATATGTTTGACCGTCAAATCGATATGATTA  >  CP000730/1420289‑1420569                                                                                                                                                  |                                                                                                                                        cGATTCATTTGAACATGATGGTACAACGTACCCTGTCGAGTATGCTACGTTGGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACcacg                                                                                                                                       <  1:13905/148‑1 (MQ=255)         ttGAACATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACata                                                                                                                               >  2:3162/1‑148 (MQ=255)          tGAACATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACatat                                                                                                                              >  1:211217/1‑148 (MQ=255)           gAACATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACAtata                                                                                                                             >  1:357404/1‑148 (MQ=255)           gAACATGATGGTACAACGTACCCNGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACAtata                                                                                                                             >  1:337074/1‑148 (MQ=255)            aaCATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATataa                                                                                                                            >  1:30218/1‑148 (MQ=255)            aaCATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATataa                                                                                                                            <  1:217299/148‑1 (MQ=255)               atgatgGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAatat                                                                                                                         <  2:348815/148‑1 (MQ=255)                   tgGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCaa                                                                                                                     >  2:77630/1‑148 (MQ=255)                   tgGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCaa                                                                                                                     >  2:172623/1‑148 (MQ=255)                     gTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGt                                                                                                                   <  1:77426/148‑1 (MQ=255)                                                      aaaaTGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTgg                                                                                  >  2:47783/1‑148 (MQ=255)                                                      aaaaTGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTgg                                                                                  >  1:206478/1‑148 (MQ=255)                                                         aTGATTATGAAGATAATAACGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGAtt                                                                               <  1:3162/148‑1 (MQ=255)                                                              tATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAAt                                                                          <  2:375408/148‑1 (MQ=255)                                                               aTGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATc                                                                         >  2:272545/1‑148 (MQ=255)                                                                   aGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTc                                                                     >  2:110512/1‑148 (MQ=255)                                                                   aGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTc                                                                     >  2:211758/1‑148 (MQ=255)                                                                   aGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTc                                                                     >  1:255994/1‑148 (MQ=255)                                                                       aataaAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCg                                                                 <  1:366987/148‑1 (MQ=255)                                                                       aataaAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCg                                                                 <  1:15467/148‑1 (MQ=255)                                                                                    aGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAGATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACa                                                    >  2:96178/1‑148 (MQ=255)                                                                                    aGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACa                                                    >  2:326547/1‑148 (MQ=255)                                                                                         aGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTc                                               <  2:291134/148‑1 (MQ=255)                                                                                              tAAAAGTTTCAAATCGTTTCGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAAAAAGAACAAATTGTAACTGATTCCCGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCacgac                                          <  2:324266/148‑2 (MQ=255)                                                                                              tAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCaagaa                                          <  2:301676/148‑1 (MQ=255)                                                                                                   gTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATCTAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAAc                                     >  1:87923/1‑148 (MQ=255)                                                                                                   gTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAAc                                     >  2:274636/1‑148 (MQ=255)                                                                                                   gTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAAc                                     >  1:241159/1‑148 (MQ=255)                                                                                                      tCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAAcgcg                                  >  1:186034/1‑148 (MQ=255)                                                                                                         aaTCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAACGCGTGa                               >  1:164591/1‑148 (MQ=255)                                                                                                            cGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAACGCGTGatat                            <  1:165351/148‑1 (MQ=255)                                                                                                                                     tCAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAACGCGTGATATGTTTGACCGTCAAATCGATATGAtt   <  1:272545/148‑1 (MQ=255)                                                                                                                                      cAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAACGCGTGATATGTTTGACCGTCAAATCGATATGATTa  >  1:221611/1‑148 (MQ=255)                                                                                                                                      cAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAACGCGTGATATGTTTGACCGTCAAATCGATATGATTa  >  1:273960/1‑148 (MQ=255)                                                                                                                                      cAGCATACTACCACGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAACGCGTGATATGTTTGACCGTCAAATCGATATGATTa  >  1:178565/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                        CGATTCATTTGAACATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGATTATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGGATTCGAAAATATCAGCATACTACCGCGGCTACATATAATATGCAAGTACAACAAGAAAAAATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAAGTAACGCGTGATATGTTTGACCGTCAAATCGATATGATTA  >  CP000730/1420289‑1420569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑