Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,530,723	G→A	T188I (ACA→ATA)	bshA ←	glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,530,723	0	G	A	100.0%	81.5 / NA	23	T188I (ACA→ATA)	bshA	glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

ACTAGGTATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACTTTTTTCAATGCCA  >  CP000730/1530593‑1530863                                                                                                                                   |                                                                                                                                             aCTAGGTATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGa                                                                                                                             <  1:350788/148‑1 (MQ=255)      gTATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTc                                                                                                                        >  1:253651/1‑148 (MQ=255)       tATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTct                                                                                                                       >  2:180126/1‑147 (MQ=255)       tATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCa                                                                                                                       >  1:54699/1‑148 (MQ=255)       tATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCa                                                                                                                       >  2:380016/1‑148 (MQ=255)       tATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCa                                                                                                                       >  2:269671/1‑148 (MQ=255)       tATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCa                                                                                                                       >  2:308444/1‑148 (MQ=255)                            aaaTGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATaa                                                                                                  <  1:211210/148‑1 (MQ=255)                                gTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTTTTTTTTTTTTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATct                                                                                              >  2:354586/1‑147 (MQ=255)                                gTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCg                                                                                              >  2:28820/1‑148 (MQ=255)                                gTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCg                                                                                              >  2:21721/1‑148 (MQ=255)                                     tataATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGtata                                                                                         <  1:206249/148‑1 (MQ=255)                                     tataATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGtata                                                                                         <  1:369/148‑1 (MQ=255)                                         ataGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAAttt                                                                                     <  2:77194/148‑1 (MQ=255)                                                cTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGtt                                                                              >  1:125167/1‑148 (MQ=255)                                                              tGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAAttt                                                                <  1:356312/148‑1 (MQ=255)                                                                                               tCATCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCacac                               <  2:349804/148‑1 (MQ=255)                                                                                                  tCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTg                            <  1:304907/148‑1 (MQ=255)                                                                                                  tCTGGTGCTATACCAAATTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTg                            <  2:166457/148‑1 (MQ=255)                                                                                                                aaaTTGTGATTTTAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACtt              >  1:21677/1‑148 (MQ=255)                                                                                                                         ttttAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACTTTTTTCAATg     <  1:164259/148‑1 (MQ=255)                                                                                                                            tAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACTTTTTTCAATGCCa  >  2:260291/1‑148 (MQ=255)                                                                                                                            tAATGCTATGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACTTTTTTCAATGCCa  >  1:253664/1‑148 (MQ=255)                                                                                                                                   |                                                                                                                                             ACTAGGTATCTTTTCACGTACTTTTGCAAATGTTTCTATAATAGTATCTATGCGTTTTACTTGTCTAAAATTCGATACATGTATTAACACTTTTTCATCTGGTGCTATACCAAATTGTGATTTTAATGCTGTGTTATGTTTAGTTGGAAACTCATTTTCACGTACAAAATTATAAATCGGTATAATTTCTTTGTTAGTTTCAATAATTTCATGTGTTTCTTGTGCTAAAGATTTACTCACACTTGTCACAATATCACTTTTTTCAATGCCA  >  CP000730/1530593‑1530863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑