Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,775,556 G→A P323L (CCT→CTT)  clpX ← S14 family endopeptidase ClpX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,775,5560GA100.0% 99.5 / NA 31P323L (CCT→CTT) clpXS14 family endopeptidase ClpX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/13);  total (18/13)

CTATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAGGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCTAATAATGCTTGTTCGT  >  CP000730/1775413‑1775697
                                                                                                                                               |                                                                                                                                             
ctATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATTTTGTTTCACAAGTGCATTTTTAAGTTg                                                                                                                                           >  2:259337/1‑148 (MQ=255)
     aTTGAACGTAAACCACGCGCACCTGTTTTTTTTTTTTTTTCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTTCGttt                                                                                                                                      >  2:204914/1‑147 (MQ=255)
     aTTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTa                                                                                                                                      >  2:301818/1‑148 (MQ=255)
           cGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGt                                                                                                                                >  2:56435/1‑148 (MQ=255)
              aaaCCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGtttt                                                                                                                             <  1:41468/148‑1 (MQ=255)
              aaaCCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGtttt                                                                                                                             <  1:50226/148‑1 (MQ=255)
                           cTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTAc                                                                                                                <  1:274496/148‑1 (MQ=255)
                                       cAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTc                                                                                                    >  1:211948/1‑148 (MQ=255)
                                       cAATTGCTTTTTCACTAATAGCTGATAAAGCTTCTTCAGTGTACTCTAACTCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTc                                                                                                    >  2:219179/1‑148 (MQ=255)
                                             cTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAAtt                                                                                              <  2:174073/148‑1 (MQ=255)
                                                         ttGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTgg                                                                                  <  2:359702/148‑1 (MQ=255)
                                                             tGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGcaca                                                                              >  1:328549/1‑148 (MQ=255)
                                                                gaaaGCTTCGTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGGATATGGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGt                                                                           >  1:78828/2‑148 (MQ=255)
                                                                           cAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTc                                                                >  2:291380/1‑148 (MQ=255)
                                                                                                 ttcTAATTCCAGCTATCGAGTATATTGTGTCACAAGTGCATTTTTAAGATGCGTTAAGATGTTTTTCAACGCAGCTACAGCTAATGTTTCTAAATTAGCTACAATTGGCACACGGCCGATAAATTCAGGAATCAAACCATAGGCCTGc                                          <  2:284419/147‑1 (MQ=255)
                                                                                                     aaTTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAAt                                      >  1:120095/1‑148 (MQ=255)
                                                                                                     aaTTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAAt                                      >  1:99387/1‑148 (MQ=255)
                                                                                                               ttttAGTATATTGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCg                            >  1:223497/1‑148 (MQ=255)
                                                                                                                         ttGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCt                  <  2:210443/148‑1 (MQ=255)
                                                                                                                         ttGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCt                  <  2:268215/148‑1 (MQ=255)
                                                                                                                         ttGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCt                  >  1:282523/1‑148 (MQ=255)
                                                                                                                         ttGTTTCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCt                  <  2:244517/148‑1 (MQ=255)
                                                                                                                              tCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCtaataa             >  1:371280/1‑148 (MQ=255)
                                                                                                                              tCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCtaataa             >  1:53521/1‑148 (MQ=255)
                                                                                                                              tCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCtaataa             >  2:346594/1‑148 (MQ=255)
                                                                                                                              tCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCtaataa             >  1:267844/1‑148 (MQ=255)
                                                                                                                              tCACAAGTGCATTTTTAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCtaataa             >  2:343405/1‑148 (MQ=255)
                                                                                                                                         tttttAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCTAATAATGCTTGTTCGt  <  1:177658/148‑1 (MQ=255)
                                                                                                                                         tttttAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCTAATAATGCTTGTTCGt  <  2:147599/148‑1 (MQ=255)
                                                                                                                                         tttttAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCTAATAATGCTTGTTCGt  <  2:188922/148‑1 (MQ=255)
                                                                                                                                         tttttAAGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCTAATAATGCTTGTTCGt  <  2:230379/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                             
CTATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAGGTTGCGTTAAGATGTTTTTCAACGCAGTTACATCTAATGTTTCTAAATTAGCTACAATTGGCACACGTCCGATAAATTCAGGAATCAAACCATAGGCTTGCAAATCTTCTGGGCGAATTTGTGCTAATAATGCTTGTTCGT  >  CP000730/1775413‑1775697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑