Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,018,727:1 +A 100% coding (192/810 nt) mgt ← transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,018,7271.A100.0% 25.0 / NA 8L64F (TTA→TTTmgttransglycosylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (4/4);  total (4/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

ATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGT  >  CP000730/2018591‑2018859
                                                                                                                                         |                                                                                                                                    
aTCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTACCATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaaaa                                                                                                                                  >  2:24970/1‑141 (MQ=255)
                  aTCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATa                                                                                                                 <  1:121889/141‑1 (MQ=255)
                   tCGTTCATCTTCCATTGAAATAAAGGCACTTTTATCATTCTCTGGCGTTTTTGTATCTGGCACAAAATGACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAAGACATGATACCAATaa                                                                                                                <  1:55425/141‑1 (MQ=255)
                    cGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTTTTTTTTCTTCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATaaa                                                                                                               >  2:56159/1‑141 (MQ=255)
                    cGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATaaa                                                                                                               >  2:117664/1‑141 (MQ=255)
                                                                   gTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAAGATTTTTAATAGTATTc                                                                >  1:74158/1‑141 (MQ=255)
                                                                      aTCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGtt                                                             >  2:73547/1‑141 (MQ=255)
                                                                                                           cTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTg                        <  1:24970/141‑1 (MQ=255)
                                                                                                                                 gtgtAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGt  <  1:102199/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
ATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGT  >  CP000730/2018591‑2018859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: