Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,318,237	T→A	100%	F124Y (TTT→TAT)	miaB →	2‑methylthioadenine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,318,237	0	T	A	100.0%	10.7 / NA	5	F124Y (TTT→TAT)	miaB	2‑methylthioadenine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (3/2);  total (3/2)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTATACTTGAAGCATTAGGCTATCAAGCAACGACTGATATTAACACTGCAGATGTTATTTTAATTAATACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTTTAGTGAAATAGGTAATTTGAAGCATTTGAAAAAAGAACGACCTGATATTTTAATCGGTGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGAATAAAATTTTAAAATCGTATCA  >  CP000730/1318131‑1318354                                                                                                           |                                                                                                                      ggTATACTTGAAGCATTAGGCTATCAAGCAACGACTGATATTAACACTGCAGATGTTATTTTAATTAATACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTATAGTGAAATAGGTAATTTGAAGCATTTGaaaaaa                                                                                     <  2:129506/141‑1 (MQ=255)                                                               aattaatACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTATAGTGAAATAGGTAATTTGAAGAAAAAAAAAAAAGAACGACCTGATATTTTAATCGGTGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGaa                       <  2:33104/141‑1 (MQ=255)                                                                attaatACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTATAGTGAAATAGGTAATTTGAAGCATTTGAAAAAAGAACGACCTGATATTTTAATCGGTGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGAAt                      >  2:113259/1‑141 (MQ=255)                                                                    atACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTATAGTGAAATAGGTAATTTGAAGCATTTGAAAAAAGAACGACCTGATATTTTAATCGGTGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGAATaaaa                  >  1:54295/1‑141 (MQ=255)                                                                                    agaAAATGCCGAGAACAAAGTGTATAGTGAAATAGGTAATTTGAAGCATTTGAAAAAAGAACGACCTGATATTTTAATCGGTGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGAATAAAATTTTAAAATCGTATCa  >  2:43462/1‑141 (MQ=255)                                                                                                           |                                                                                                                      GGTATACTTGAAGCATTAGGCTATCAAGCAACGACTGATATTAACACTGCAGATGTTATTTTAATTAATACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTTTAGTGAAATAGGTAATTTGAAGCATTTGAAAAAAGAACGACCTGATATTTTAATCGGTGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGAATAAAATTTTAAAATCGTATCA  >  CP000730/1318131‑1318354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑