| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,380,750 | C→T | 28.6% | intergenic (+2847/‑328) | sbcC → / → acnA | exodeoxyribonuclease SbcC/aconitate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,380,750 | 0 | C | T | 28.6% | 18.3 / 7.0 | 14 | intergenic (+2847/‑328) | sbcC/acnA | exodeoxyribonuclease SbcC/aconitate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (6/4); new base T (2/2); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
CCAACACAGAAGCTGACGAAAAGTCAGCTTACAATAATGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACATTGAAAAAAATATTAAAGTTTGGTGCCATAATTTTCTTTCTGCTCAATGAAATAATACGTTTAAAAATT > CP000730/1380619‑1380860 | ccAACACAGAAGCTGACGAAAAGTCAGCTTACAATAATGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATc > 1:118200/1‑141 (MQ=35) cacaGAAGCTGACGAAAAGTCAGCTTACAATAATGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCAttt > 1:9258/1‑141 (MQ=39) aaGCTGACGAAAAGTCAGCTTACAATAATGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTc < 2:66030/141‑1 (MQ=38) gCTGACGAAAAGTCAGCTTACAATAATGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTccc < 2:73878/141‑1 (MQ=38) gCTGACGAAAAGTCAGCTTACAATAATGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTccc < 1:69146/141‑1 (MQ=38) gCTTACAATAATGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATCAAAAGa < 2:50011/141‑1 (MQ=255) aataatGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGTGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACa > 1:2373/1‑141 (MQ=255) aataatGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGTGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACa > 1:2368/1‑141 (MQ=255) ggATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGTGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACATTGAAAAAAATATTAAAGTTTGGTGCCATAATTTTCTTTCTg < 2:2368/141‑1 (MQ=255) ggATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGTGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAAAGACAATAAAAAAAATATTAAAGTTTGGTGCCATAATTTTCTTTCTg < 2:2373/141‑1 (MQ=255) gATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACATTGAAAAAAATATTAAAGTTTGGTGCCATAATTTTCTTTCTGc > 1:6970/1‑141 (MQ=255) cAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACATTGAAAAAAATATTAAAGTTTGGTGCCATAATTTTCTTTCTGCTCAATg > 1:52515/1‑141 (MQ=255) aaGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACATTGAAAAAAATATTAAAGTTTGGTGCCATAATTTTCTTTCTGCTCAATGAAATAATACGTTTAAAaat > 1:91021/1‑141 (MQ=255) aGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACATTGAAAAAAATATTAAAGTTTGGTGCCATAATTTTCTTTCTGCTCAATGAAATAATACGTTTAAAaatt > 2:89992/1‑141 (MQ=255) | CCAACACAGAAGCTGACGAAAAGTCAGCTTACAATAATGTGCAAGTTGGGGATGGGCCCCAACAAAGAGAAATTGGATTCCCAATTTCTACAGACAATGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCATCAAAAGAATGACATTGAAAAAAATATTAAAGTTTGGTGCCATAATTTTCTTTCTGCTCAATGAAATAATACGTTTAAAAATT > CP000730/1380619‑1380860 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |