| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,434,045 | C→T | 24.4% | G62G (GGC→GGT) | yleF → | RpiR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,434,045 | 0 | C | T | 24.4% | 26.5 / 4.4 | 16 | G62G (GGC→GGT) | yleF | RpiR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (7/5); new base T (2/2); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATGAAAAAAAGATAGCTAAATTTATACTAAATTCACCTCAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTAATGAATAACGAAAGCACAGAATCTTTAAGAAC > CP000730/2433910‑2434149 | acaagAAAAAAAGATAGCTAAATTTATACTAAATTCACCTCAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGttt < 2:109591/137‑1 (MQ=255) gCTAAATTTATACTAAATTCACCTCAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAaaata > 2:96285/1‑141 (MQ=255) gCTAAATTTATACTAAATTCACCTCAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAaaata > 2:71739/1‑141 (MQ=255) ccTCAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGTGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCt > 1:5425/1‑141 (MQ=255) ccTCAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATAAGTCATTCGATTCAGCAAGAAAATTACTGATGGTGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCt > 1:5488/1‑141 (MQ=255) cTCAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAACATGTTCCTa > 2:47150/1‑141 (MQ=255) cAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTaaa > 2:20089/1‑141 (MQ=255) ggTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGcatc > 1:62325/1‑141 (MQ=255) gACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTa < 2:103388/141‑1 (MQ=255) gACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTa < 2:103381/141‑1 (MQ=255) aGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTAATGaa > 2:64390/1‑141 (MQ=255) gCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGTGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTAATGAATAACg < 2:5425/141‑1 (MQ=255) gCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGTGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTAATGAATAACg < 2:5488/141‑1 (MQ=255) atatTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTAATGAATAACGAAAGCACAg > 2:133950/1‑141 (MQ=255) catcatCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTAATGAATAACGAAAGCACAGAATCTTTaa < 1:20089/141‑1 (MQ=255) atcaGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTAATGAATAACGAAAGCACAGAATCTTTAAGAAc < 1:89907/141‑1 (MQ=255) | AAATGAAAAAAAGATAGCTAAATTTATACTAAATTCACCTCAAAAGGTGATTAAAATGAGGTCTCAAGACTTAGCAAGCTTATTAGATATTAGTACATCATCAGTCATTCGATTCAGCAAGAAAATTACTGATGGCGGTTTTCACGACTTGAAAATAAATATATCTAAATATGTTCCTAAAGCATCATCAATTTACAATGTTGAATTAATGAATAACGAAAGCACAGAATCTTTAAGAAC > CP000730/2433910‑2434149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |