New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 802131 =97 (1.080)3 (0.040) 3/254 15.4 3.1% intergenic (+372/+252) USA300HOU_0748/dgkA1 hypothetical protein/possible diacylglycerol kinase
?CP000730 802180 = 98 (1.190)intergenic (+421/+203) USA300HOU_0748/dgkA1 hypothetical protein/possible diacylglycerol kinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGTTTCAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGATTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/802280‑802131
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcatccgatttTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAATATAAGGTG  >  CP000730/802180‑802319
                                                                                                                                                                                                                                                                                                  
TGTTTCAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTC                                                                                                                                                       <  1:655234/141‑1
 GTTTCAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCA                                                                                                                                                      <  2:646868/141‑1
 GTTTCAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATACTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCA                                                                                                                                                      <  2:666853/141‑1
  TTTCAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGAAGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATAGGATGACGTGTAATCTGCCATAGATTAACACATTCAT                                                                                                                                                     >  2:315822/1‑141
   TTCAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATC                                                                                                                                                    <  1:327876/141‑1
    TCAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCC                                                                                                                                                   <  1:678540/141‑1
     CAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCG                                                                                                                                                  >  2:962099/1‑141
      AAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGA                                                                                                                                                 <  1:516644/141‑1
      AAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGA                                                                                                                                                 <  2:560074/141‑1
      AAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGA                                                                                                                                                 >  2:302192/1‑141
       AAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGAT                                                                                                                                                >  1:81545/1‑141
       AAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGAT                                                                                                                                                >  2:737773/1‑141
        AAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGATT                                                                                                                                               >  1:943773/1‑141
         AACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGATTT                                                                                                                                              >  1:303818/1‑141
                                                                                                   AAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGATTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTG                                                    >  1:149351/1‑141
                                                                                                            ATGACGTGTAATCTGCCATAGATTAACACATTCATCCGATTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAA                                           >  2:301626/1‑141
                                                                                                             TGACATGTAATCTGCCATAGATTAACACATGCATCCGATTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATATTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAAC                                          >  1:688490/1‑141
                                                                                                                                           TCATCCGATTTTTTATAGTATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTTAAACAGGGTAATAGCTTAAAGCTAATAAAAACGA            <  1:465066/141‑1
                                                                                                                                             ATCCGATTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAAT          <  1:421429/141‑1
                                                                                                                                                CGATTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAATATA       <  2:351347/141‑1
                                                                                                                                                 GATTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAATATAA      >  2:895504/1‑141
                                                                                                                                                   TTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAATATAAGG    <  1:281364/141‑1
                                                                                                                                                   TTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAATATAAGG    >  1:752338/1‑141
                                                                                                                                                   TTTTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAATATAAGG    >  1:802903/1‑141
                                                                                                                                                     TTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAATATAAGGTG  <  1:98848/141‑1
                                                                                                                                                                                                                                                                                                  
TGTTTCAAAAACAGACATATAATTTTGGCGCATGCCACTAAAATGATTGATGTTGTATTTTAAATCATAGTGGTTTATGCGTCTTTTTCAAATTCTATAAAAAATCGGATGACGTGTAATCTGCCATAGATTAACACATTCATCCGATTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/802280‑802131
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcatccgatttTTTATAGAATTTGAAAAAGACGCATAAACCACTATGATTTAAAATACAACATCAATCATTTTAGTGGCATGCGCCAAAATTATATGTCTGTTTTTGAAACAGGGTAATAGCTTAAAGCTAATAAAAACGAATATAAGGTG  >  CP000730/802180‑802319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.