Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	638,614	G→T	T14K (ACA→AAA)	thiD ←	phosphomethylpyrimidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	638,614	0	G	T	100.0%	311.8 / NA	84	T14K (ACA→AAA)	thiD	phosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (43/41);  total (43/41)

CATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAT  >  CP000730/638477‑638750                                                                                                                                          |                                                                                                                                          cATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        >  2:797042/1‑141 (MQ=255) cATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        >  1:233548/1‑141 (MQ=255) cATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt                                                                                                                                        <  1:126678/141‑1 (MQ=255)     cATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGa                                                                                                                                    >  2:6339/1‑141 (MQ=255)      aTTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGaa                                                                                                                                   >  1:287762/1‑141 (MQ=255)         ggTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCg                                                                                                                                <  2:896990/141‑1 (MQ=255)           tAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGc                                                                                                                              <  1:544798/141‑1 (MQ=255)             aTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCaa                                                                                                                            >  1:651047/1‑141 (MQ=255)             aTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCaa                                                                                                                            <  1:588755/141‑1 (MQ=255)             aTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCaa                                                                                                                            >  1:135676/1‑141 (MQ=255)              tGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAt                                                                                                                           <  1:819296/141‑1 (MQ=255)              tGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAt                                                                                                                           >  2:253642/1‑141 (MQ=255)              tGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTAGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAt                                                                                                                           <  2:690906/141‑1 (MQ=255)               ggTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAAtt                                                                                                                          >  2:921645/1‑141 (MQ=255)                gtgtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTg                                                                                                                         >  1:166799/1‑141 (MQ=255)                  gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGtt                                                                                                                       <  1:118311/141‑1 (MQ=255)                  gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGtt                                                                                                                       <  1:907384/141‑1 (MQ=255)                  gtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGtt                                                                                                                       <  2:826988/141‑1 (MQ=255)                     aCATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATAGCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑aaa                                                                                                                   <  1:512439/141‑1 (MQ=255)                         cGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAActt                                                                                                               >  1:114774/1‑141 (MQ=255)                         cGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAActt                                                                                                               >  2:615670/1‑141 (MQ=255)                          gtgtGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAActtt                                                                                                              >  2:118696/1‑141 (MQ=255)                              gACCATGTATCTTTATCCAGAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCCGCACTTTTGTCAGACCGGGCAATTGTT‑AAAActttcttt                                                                                                          <  2:323786/141‑1 (MQ=255)                                      aTCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCAt                                                                                                  <  1:406209/141‑1 (MQ=255)                                           tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGc                                                                                             <  1:671441/141‑1 (MQ=255)                                           tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGAAGCCGCTGCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGc                                                                                             <  1:17593/141‑1 (MQ=255)                                            aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCt                                                                                            >  2:657534/1‑141 (MQ=255)                                                 tAGTAACGATGGCAGTTAAAGCGACCATGCAATACGTATCTAATTCTTGGAACGTTGTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACt                                                                                       <  1:11676/141‑1 (MQ=255)                                                  aGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTc                                                                                      >  1:246431/1‑141 (MQ=255)                                                   gTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTcc                                                                                     >  1:819235/1‑141 (MQ=255)                                                     aaCGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTa                                                                                   >  2:883723/1‑141 (MQ=255)                                                      aCGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTAc                                                                                  >  2:449724/1‑141 (MQ=255)                                                         aTGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACata                                                                               >  2:515344/1‑141 (MQ=255)                                                           ggCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataat                                                                             <  2:167426/141‑1 (MQ=255)                                                             cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           <  2:630546/141‑1 (MQ=255)                                                             cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           >  1:872582/1‑141 (MQ=255)                                                             cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           >  2:13154/1‑141 (MQ=255)                                                             cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           >  1:464755/1‑141 (MQ=255)                                                               gTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataata                                                                         <  1:412365/141‑1 (MQ=255)                                                                 tAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATAtt                                                                       >  1:195345/1‑141 (MQ=255)                                                                  aaaGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTg                                                                      <  1:407662/141‑1 (MQ=255)                                                                     gCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTAt                                                                   >  1:739754/1‑141 (MQ=255)                                                                         ccATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcat                                                               >  2:857863/1‑141 (MQ=255)                                                                         ccATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcat                                                               >  1:272685/1‑141 (MQ=255)                                                                            tGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGTCATTGAGCTTCACTCCTACATAATAATATTGTATTcatcat                                                            >  1:503008/1‑141 (MQ=255)                                                                            tGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTcatcat                                                            >  1:817392/1‑141 (MQ=255)                                                                             gCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTTAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcata                                                           <  2:847630/141‑1 (MQ=255)                                                                             gCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcata                                                           >  1:415206/1‑141 (MQ=255)                                                                                     tATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttttt                                                   <  1:755094/141‑1 (MQ=255)                                                                                     tATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttttt                                                   >  1:410951/1‑141 (MQ=255)                                                                                      aTCTAATTCTTGGAACGTTTTCAAATCTGCTTGGATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTa                                                  >  1:502854/1‑141 (MQ=255)                                                                                      aTCTAATTCTTGGAACGTTTTCAAATCTGCTTGGATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTa                                                  >  1:502768/1‑141 (MQ=255)                                                                                         tAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCGGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAAcc                                               <  1:44689/141‑1 (MQ=255)                                                                                            ttCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTaa                                            <  1:913239/141‑1 (MQ=255)                                                                                             tCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTTTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAAt                                           <  1:867881/141‑1 (MQ=255)                                                                                              cTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAAtt                                          >  1:183710/1‑141 (MQ=255)                                                                                              cTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAAtt                                          <  2:246442/141‑1 (MQ=255)                                                                                               ttGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTg                                         <  1:898638/141‑1 (MQ=255)                                                                                               ttGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTg                                         >  2:423168/1‑141 (MQ=255)                                                                                               ttGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTg                                         <  1:377447/141‑1 (MQ=255)                                                                                                tGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGa                                        >  1:886985/1‑141 (MQ=255)                                                                                                 ggAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTGATTGaa                                       <  1:314756/141‑1 (MQ=255)                                                                                                 ggAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGaa                                       <  1:166559/141‑1 (MQ=255)                                                                                                     cGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAat                                   >  2:171441/1‑141 (MQ=255)                                                                                                          tCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTaa                              >  1:186114/1‑141 (MQ=255)                                                                                                              aTCTGCTTGCATACCTGCTCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCa                           <  2:673568/141‑1 (MQ=255)                                                                                                              aTCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCAt                          <  1:44690/141‑1 (MQ=255)                                                                                                              aTCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCAt                          >  1:799267/1‑141 (MQ=255)                                                                                                               tCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCAtt                         <  1:667443/141‑1 (MQ=255)                                                                                                                cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                        <  2:923882/141‑1 (MQ=255)                                                                                                                cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                        <  1:738023/141‑1 (MQ=255)                                                                                                                cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                        <  1:402498/141‑1 (MQ=255)                                                                                                                cTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTc                        <  1:219296/141‑1 (MQ=255)                                                                                                                   cTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAat                     <  1:883723/141‑1 (MQ=255)                                                                                                                    ttGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTAc                                                                                  >  1:206791/1‑79 (MQ=255)                                                                                                                    ttGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTAc                                                                                  <  2:206791/79‑1 (MQ=255)                                                                                                                     tGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAatat                   >  1:720085/1‑141 (MQ=255)                                                                                                                     tGCATACCTGCGCCAGCACGTTTGTCAGAACCGGCAATTGAG‑AGAACTTGCTGTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACGTAATTGAAAAAGGTTAAGCATTAAatat                   >  2:577702/1‑141 (MQ=255)                                                                                                                        aTACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtg                >  2:477395/1‑141 (MQ=255)                                                                                                                         tACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtga               >  1:523089/1‑141 (MQ=255)                                                                                                                         tACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtga               <  1:3676/141‑1 (MQ=255)                                                                                                                          aCCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtgat              <  2:21760/141‑1 (MQ=255)                                                                                                                            cTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtgatga            <  2:894341/141‑1 (MQ=255)                                                                                                                                   aGCACTTTTGTCAGAACCGGCACTTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGTAAAATATTAAGCATTCAATATTTGATGAttgttta     >  2:920676/1‑139 (MQ=255)                                                                                                                                   aGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGa     >  1:18351/1‑141 (MQ=255)                                                                                                                                   aGCACTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGa     <  2:764604/141‑1 (MQ=255)                                                                                                                                      aCTTTTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAt  >  2:854138/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                          CATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTT‑AAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAT  >  CP000730/638477‑638750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑