Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,709,600	(T)5→4	coding (1327/1812 nt)	oatA ←	possible acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,709,596	0	T	.	100.0%	121.6 / NA	26	coding (1331/1812 nt)	oatA	possible acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/12);  total (14/12)

TATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAT  >  CP000730/2709456‑2709728                                                                                                                                             |                                                                                                                                     tataTTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACt                                                                                                                                      <  2:331207/141‑1 (MQ=255)      tGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaa                                                                                                                                 >  1:338984/1‑139 (MQ=255)      tGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaa                                                                                                                                 <  1:328741/141‑3 (MQ=255)       gCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaat                                                                                                                                >  1:276296/1‑138 (MQ=255)        cGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaata                                                                                                                               <  1:287700/141‑5 (MQ=255)                  aTTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGc                                                                                                                    >  1:217578/1‑141 (MQ=255)                    tGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTa                                                                                                                  >  1:144142/1‑141 (MQ=255)                       tgtAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTc                                                                                                               <  2:203136/141‑1 (MQ=255)                                    aGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTGTAATATCCTCTTTGCTATTCGCTGTCTGCTTAt                                                                                                  <  2:266089/141‑1 (MQ=255)                                     gTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATc                                                                                                 >  2:41719/1‑141 (MQ=255)                                           gTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTg                                                                                           <  1:275876/141‑1 (MQ=255)                                            tCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGc                                                                                          <  2:277019/141‑1 (MQ=255)                                               aaCTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTa                                                                                       >  1:158799/1‑141 (MQ=255)                                                        aTCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTa                                                                              >  1:126349/1‑141 (MQ=255)                                                         tCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTaa                                                                             >  1:273906/1‑141 (MQ=255)                                                           aaTTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACg                                                                           <  1:407614/141‑1 (MQ=255)                                                              ttGTGCATTTGGTATTCTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTACCGACt                                                                        <  2:7337/141‑1 (MQ=255)                                                                    aTTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTttc                                                                  <  2:223633/141‑1 (MQ=255)                                                                         gTATTTTCTTAGTAAAGACATGACCAATATCCACCATGACCGATTCACCATTTAAGAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGTCTTTTttcttctt                                                             >  2:246625/1‑139 (MQ=255)                                                                             tttCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCgttgtt                                                         >  1:361854/1‑141 (MQ=255)                                                                              ttCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGttt                                                        >  1:373682/1‑141 (MQ=255)                                                                                  tAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTaaa                                                    >  1:287765/1‑141 (MQ=255)                                                                                              tACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttctt                                        <  2:239267/141‑1 (MQ=255)                                                                                              tACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttctt                                        >  2:21019/1‑141 (MQ=255)                                                                                              tACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTCTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttctt                                        >  2:390011/1‑141 (MQ=255)                                                                                                    tatCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtcttt                                  <  1:215096/141‑1 (MQ=255)                                                                                                    tatCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtcttt                                  <  1:408834/141‑1 (MQ=255)                                                                                                                   aGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCCTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtg                   <  2:7096/141‑1 (MQ=255)                                                                                                                                tAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGt      >  1:275895/1‑141 (MQ=255)                                                                                                                                tAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGt      >  1:130838/1‑141 (MQ=255)                                                                                                                                    ggtgATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAt  <  2:151019/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                     TATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAT  >  CP000730/2709456‑2709728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑