Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,018,733	(A)6→7	coding (186/810 nt)	mgt ←	transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,018,727	1	.	A	100.0%	128.3 / NA	37	L64F (TTA→TTT)	mgt	transglycosylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (14/23);  total (14/23)

CGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑AAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGA  >  CP000730/2018588‑2018856                                                                                                                                             |                                                                                                                                  cGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCTTTTTTTTTTTGTTCATCCACATTATCTCGTGTAGAT‑a                                                                                                                                  >  2:271203/1‑141 (MQ=255)   aaTCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaa                                                                                                                                <  1:136910/141‑1 (MQ=255)                 gTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATATTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATAc                                                                                                                   >  1:298098/1‑141 (MQ=255)                  tAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATAcc                                                                                                                  >  2:386552/1‑141 (MQ=255)                     caTCGTTCATCTTCCACTGACATCAAGGCACCTTTAACATACTATGGCCTGTTATCAGCTGCCACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAAt                                                                                                               <  2:86652/140‑1 (MQ=255)                      aTCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATa                                                                                                              <  1:65953/141‑1 (MQ=255)                           aaaaaatacATTGAAATAAAGGCACCTTTAACATAATCTGGCATGTTATCAGCCGACACAAAACTACTTTTAATTTAAAATTTTCTTAGTTCATCCACATTATAGAAAAAAAAAAAAAAAATACATGATACCAATAAACaa                                                                                                         <  2:301738/133‑1 (MQ=255)                                ttCCATTGAAATAAAGGCACCTTTAACATCCTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGa                                                                                                    <  1:413861/141‑1 (MQ=255)                                ttCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGa                                                                                                    <  1:149902/141‑1 (MQ=255)                                       aaaaTAAAGGAAGCTTTAAGATACTTTGGCATGATATCAGCAGACACAAAACTACTTTTATTTTAAATTTTTCTTAGTTCATCCACATTATAAAAAAAAAAAAAAAAAATACATGATACCAATAAACAATGCGATAATGAt                                                                                             <  2:300622/140‑1 (MQ=255)                                          aTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAg                                                                                          >  2:227015/1‑141 (MQ=255)                                          aTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAg                                                                                          <  1:54279/141‑1 (MQ=255)                                             aaGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAAt                                                                                       >  1:110315/1‑141 (MQ=255)                                                   ccTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGCTTAGAATGGTtaa                                                                                 >  1:353236/1‑141 (MQ=255)                                                             aCTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTTTTTTTTTTTCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATAttttt                                                                       >  2:98914/1‑141 (MQ=255)                                                              ctctGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTa                                                                      <  1:180664/141‑1 (MQ=255)                                                                   gCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTTTTTTTTTTTCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGt                                                                 >  2:262831/1‑141 (MQ=255)                                                                       gTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTc                                                             <  1:302610/141‑1 (MQ=255)                                                                        ttATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTct                                                            >  2:9868/1‑140 (MQ=255)                                                                        ttATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCg                                                            >  1:169147/1‑141 (MQ=255)                                                                          aTCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaataa                                                                              >  2:113698/1‑121 (MQ=255)                                                                          aTCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaataa                                                                              <  1:113698/121‑1 (MQ=255)                                                                           tCAGCTGACACAAGACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGttt                                                         <  2:5340/141‑1 (MQ=255)                                                                                  acacAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttttt                                                  >  2:330803/1‑141 (MQ=255)                                                                                       aaaCTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttctt                                             >  1:207635/1‑141 (MQ=255)                                                                                         actactTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttctttt                                           <  1:138568/141‑1 (MQ=255)                                                                                          ttactTTTATTTTCAATTTTTCTTATTTCATCCACATTATCGCTTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTTATAGTATTCGTTTACTTtttttcttgtt                                          <  2:350358/140‑1 (MQ=255)                                                                                                        aaTTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTTGGCGGTTTGCCaa                            >  2:116333/1‑141 (MQ=255)                                                                                                         aTTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAAc                           <  1:330803/141‑1 (MQ=255)                                                                                                             ttCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGt                       <  1:269805/141‑1 (MQ=255)                                                                                                             ttCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGt                       <  1:73415/141‑1 (MQ=255)                                                                                                                 tAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGat                   <  1:11610/141‑1 (MQ=255)                                                                                                                         ccACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGta           <  1:85992/141‑1 (MQ=255)                                                                                                                          cacaTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtat          <  2:110315/141‑1 (MQ=255)                                                                                                                            caTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatta        <  1:156442/141‑1 (MQ=255)                                                                                                                            caTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatta        >  1:239251/1‑141 (MQ=255)                                                                                                                              ttATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATtata      <  1:236896/141‑1 (MQ=255)                                                                                                                                aTCGCGTGTAAATAAAAAAATATATGATACCAATAAATGTTGGGATAATGTTTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTATTTTTTTTCTTTTTTCGCGGTTAGGCAACTATTTTATGATATGTATTTTAGt    <  2:186306/141‑1 (MQ=255)                                                                                                                                  cgcgTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGa  <  2:146361/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                  CGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑AAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGA  >  CP000730/2018588‑2018856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑