New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 22553937 (0.970)4 (0.110) 4/262 5.6 9.7% coding (626/897 nt) murQ possible sugar phosphate isomerase
?CP000730 = 225543 39 (1.080)coding (630/897 nt) murQ possible sugar phosphate isomerase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCG  >  CP000730/225410‑225672
                                                                                                                                 |                                                                                                                                     
aaCATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCAttgtgatggttg                                                                                                                            <  1:226712/141‑12 (MQ=255)
  cATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAAtggttggt                                                                                                                          <  2:393528/141‑1 (MQ=255)
  cATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAAtggttggt                                                                                                                          <  2:147448/141‑1 (MQ=255)
       aGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCgg                                                                                                                     <  1:69526/141‑1 (MQ=255)
             aaGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAACAGTTAATATTAACCAGGACGTCAACCAGCGCAATGGTTGGTGGCGGAAAAGt                                                                                                               >  1:391712/1‑141 (MQ=255)
                  aaaTTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACg                                                                                                          >  2:32490/1‑141 (MQ=255)
                      tGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATaa                                                                                                      <  2:55937/141‑1 (MQ=255)
                      tGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCAAAAAAGTTAATTTTAAATATGATTTCAACCCTCACAATTGTTGGTGTCGGAAAAGTTTACGATaa                                                                                                      <  1:268049/141‑1 (MQ=255)
                         gcAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCt                                                                                                   <  1:97067/141‑1 (MQ=255)
                           aGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCa                                                                                                 >  2:237040/1‑141 (MQ=255)
                            gTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCAt                                                                                                >  2:279676/1‑141 (MQ=255)
                            gTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCAt                                                                                                >  2:318408/1‑141 (MQ=255)
                               tCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCAtgat                                                                                             <  2:170091/141‑1 (MQ=255)
                                    tAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATg                                                                                        <  1:31246/141‑1 (MQ=255)
                                     aGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGt                                                                                       <  1:90340/141‑1 (MQ=255)
                                        aGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTaa                                                                                    <  1:357597/141‑1 (MQ=255)
                                         gTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTaaa                                                                                   >  1:200511/1‑141 (MQ=255)
                                                   gTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATc                                                                         <  1:281147/141‑1 (MQ=255)
                                                   gTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATc                                                                         <  1:71950/141‑1 (MQ=255)
                                                   gTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATc                                                                         <  1:281124/141‑1 (MQ=255)
                                                   gTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATc                                                                         <  2:145152/141‑1 (MQ=255)
                                                       aGAAGTATTAACTGGTTCAACACGGTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCaaaa                                                                     <  2:388126/141‑1 (MQ=255)
                                                                 aCTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGAc                                                           >  1:353692/1‑141 (MQ=255)
                                                                  cTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAAACTTAAATATCATTTCAACCAGCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCCCATCATTGATGTTAAAGCAACCAATCAAAAACTGAGCGAcc                                                          >  2:348537/1‑141 (MQ=255)
                                                                        cAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAg                                                    >  2:66102/1‑141 (MQ=255)
                                                                          acacGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTg                                                  >  1:293361/1‑141 (MQ=255)
                                                                             cGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGt                                               <  1:122503/141‑1 (MQ=255)
                                                                             cGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGt                                               <  2:408815/141‑1 (MQ=255)
                                                                             cGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGt                                               <  1:279676/141‑1 (MQ=255)
                                                                                    aGTCAGTTACAGCACAAAATTTAATTTTAACTATTATTTCAACCATCACAATTGTTAGTTTCGGAAAAGTTTACGATAACCTCATAATTAATGTTAAAACAACCAATCAAAAACTTATCGACCGTTCAGAGCGTAATATTc                                        <  2:22755/141‑1 (MQ=255)
                                                                                                cacaAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTACTCAAGAAATAtgtg                            <  1:66102/141‑1 (MQ=255)
                                                                                                         tAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACat                   >  1:87294/1‑141 (MQ=255)
                                                                                                                 aaTATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATCTGATGaa           <  1:327092/141‑1 (MQ=255)
                                                                                                                 aaTATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGaa           <  2:143751/141‑1 (MQ=255)
                                                                                                                   tatGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGc         <  2:309293/141‑1 (MQ=255)
                                                                                                                     tGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCaa       >  2:77133/1‑141 (MQ=255)
                                                                                                                          tCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCg  >  1:155072/1‑141 (MQ=255)
                                                                                                                          tCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCg  >  2:70609/1‑141 (MQ=255)
                                                                                                                                 |                                                                                                                                     
AACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATCACAATGGTTGGTGTCGGAAAAGTTTACGATAACCTCATGATTGATGTTAAAGCAACCAATCAAAAACTGATCGACCGTTCAGTGCGTATTATTCAAGAAATATGTGCTATCACATATGATGAAGCAATGGCG  >  CP000730/225410‑225672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.