Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,238,307	C→T	Q141* (CAA→TAA)	yloV →	glycerone kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,238,307	0	C	T	100.0%	125.8 / NA	38	Q141* (CAA→TAA)	yloV	glycerone kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/24);  total (14/24)

AAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTTGATA  >  CP000730/1238174‑1238442                                                                                                                                      |                                                                                                                                        aaaTATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTATAGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAgaaaa                                                                                                                                  <  2:348524/141‑5 (MQ=255)      ttGAAAGTGAATCGGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAg                                                                                                                             <  2:138681/141‑1 (MQ=255)       tGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGa                                                                                                                            <  1:157392/141‑1 (MQ=255)              gAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCa                                                                                                                     >  1:134897/1‑141 (MQ=255)               aaTCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCaa                                                                                                                    <  1:127119/141‑1 (MQ=255)                        ttaattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTg                                                                                                           <  2:319012/141‑1 (MQ=255)                          aattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGaa                                                                                                         <  1:10576/141‑1 (MQ=255)                               aaaaTTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTg                                                                                                    <  2:410327/141‑1 (MQ=255)                                     gTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACGGAACATTGTATAGa                                                                                              >  2:17132/1‑141 (MQ=255)                                        aGCTGAAAGTTTTCAAGCTGGTTTTGAAACGGCATATAAAGCTGTTAGGAAACCAGGTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCTATAGAAAAATCAAATAATACTGAAGATTGTAGAGAAtt                                                                                           >  2:205609/1‑141 (MQ=255)                                        aGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGtatatagac                                                                                           >  1:125158/1‑136 (MQ=255)                                        aGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAAtt                                                                                           >  2:336836/1‑141 (MQ=255)                                             aaaGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATgg                                                                                      <  1:262777/141‑1 (MQ=255)                                               aGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAg                                                                                    <  2:213468/141‑1 (MQ=255)                                                           gtcgttgatctggCAGATAAAGCTGTGATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTAAATTATTGTa                                                                        <  2:117908/130‑1 (MQ=255)                                                           ggTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTa                                                                        <  1:75516/141‑1 (MQ=255)                                                           ggTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTa                                                                        >  1:361545/1‑141 (MQ=255)                                                           ggTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTa                                                                        <  1:265416/141‑1 (MQ=255)                                                           ggTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTa                                                                        <  1:349075/141‑1 (MQ=255)                                                               ttGAAACGGCATATAACGCTGTTATGAACACAATTGAAGATACAAGACTTACAGTTGCAAAAGATGCTGCGTACGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAg                                                                    >  2:101160/1‑141 (MQ=255)                                                                                   gTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAGGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAac                                                >  1:324803/1‑141 (MQ=255)                                                                                   gTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAac                                                <  2:274961/141‑1 (MQ=255)                                                                                      aTGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCACATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAacaca                                             <  1:17132/141‑1 (MQ=255)                                                                                       tGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAacacac                                            >  1:228801/1‑141 (MQ=255)                                                                                       tGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAacacac                                            <  2:187336/141‑1 (MQ=255)                                                                                          aaCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCaa                                         <  1:329972/141‑1 (MQ=255)                                                                                           aCCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTTCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCaaa                                        <  2:54863/141‑1 (MQ=255)                                                                                               gaagAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACtta                                    >  2:391890/4‑141 (MQ=255)                                                                                                   aaGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAg                                <  2:130740/141‑1 (MQ=255)                                                                                                   aaGGTACAAAACTTACAGTGGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATAAATCACTTGAAAACACACCCAACTTATTAg                                <  2:130644/141‑1 (MQ=255)                                                                                                      gTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTg                             >  2:58872/1‑141 (MQ=255)                                                                                                      gTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTg                             <  1:249468/141‑1 (MQ=255)                                                                                                              cTTACAGTTGCAAAAGATGCTGCGTAAGCTGAAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTaaa                     <  2:186401/141‑1 (MQ=255)                                                                                                                 aCAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAaagaa                  >  2:180262/1‑141 (MQ=255)                                                                                                                 aCAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAaagaa                  >  2:49967/1‑141 (MQ=255)                                                                                                                   aGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGt                <  2:357267/141‑1 (MQ=255)                                                                                                                        aaaaaGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGgtg           <  2:304436/140‑1 (MQ=255)                                                                                                                                 ctgcGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCGAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGCGTTGTTGATa  >  2:397529/1‑141 (MQ=255)                                                                                                                                      |                                                                                                                                        AAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTTGATA  >  CP000730/1238174‑1238442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑