Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,274,212	A→T	K205N (AAA→AAT)	codY →	CodY family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,274,212	0	A	T	100.0%	113.9 / NA	34	K205N (AAA→AAT)	codY	CodY family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/15);  total (19/15)

GAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTC  >  CP000730/1274078‑1274344                                                                                                                                       |                                                                                                                                     gAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCa                                                                                                                                <  1:328751/141‑1 (MQ=255)   aGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACATGGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTCTTAATCGCATCAAATGTTGCAGa                                                                                                                              <  2:351670/141‑1 (MQ=255)    gTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGAt                                                                                                                             <  1:135565/141‑1 (MQ=255)         aaaaGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGt                                                                                                                        <  2:66214/141‑1 (MQ=255)           aagaagCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTg                                                                                                                      >  2:210933/1‑141 (MQ=255)             gaagCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGt                                                                                                                    >  1:323145/1‑141 (MQ=255)              aagCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTa                                                                                                                   <  2:113211/141‑1 (MQ=255)                gcgcgcGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTAtt                                                                                                                 <  1:388705/141‑1 (MQ=255)                   cgcgATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACt                                                                                                              >  1:368639/1‑141 (MQ=255)                        tAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATc                                                                                                         <  1:343496/141‑1 (MQ=255)                            gctgctATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTa                                                                                                     >  1:177287/1‑141 (MQ=255)                               gctATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTACTCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAAtt                                                                                                  >  1:225480/1‑141 (MQ=255)                               gctATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAAtt                                                                                                  >  1:194969/1‑141 (MQ=255)                                ctATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAATCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTg                                                                                                 <  1:20786/141‑1 (MQ=255)                                  aTTACAATGGCAATTAATTCATTATCTTATTCTGAAAAATAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTa                                                                                               <  1:18215/141‑1 (MQ=255)                                           gCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAATGTCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTa                                                                                      >  2:245900/1‑141 (MQ=255)                                                         aTCGTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAg                                                                        <  1:311760/141‑1 (MQ=255)                                                                tCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGgt                                                                 <  1:322691/141‑1 (MQ=255)                                                                 cTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGgtg                                                                <  2:102632/141‑1 (MQ=255)                                                                   gAAAAAGAAGCGATGGAACATATCTTTGAAGAACTTGGCGGAACGGAAGGTCTATTTATCGCATCAGATGTTGCAGATAGAGTTGGGATTACTAGATCTGTAAATGTAAAGGCACTGCGTTAGTTAGGCAGTGCGGGTGTa                                                              >  2:167554/1‑141 (MQ=255)                                                                     aaaaGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAAt                                                            >  1:200709/1‑141 (MQ=255)                                                                           agCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATc                                                      >  1:218123/1‑141 (MQ=255)                                                                           agCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATc                                                      >  1:217990/1‑141 (MQ=255)                                                                           agCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATc                                                      >  1:217981/1‑141 (MQ=255)                                                                                  gAACATATCTTTGAAGAACTTGGCGGTACGGAATGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCt                                               >  1:248330/1‑141 (MQ=255)                                                                                               aagaaCTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAg                                  >  1:193120/1‑141 (MQ=255)                                                                                                  aaCTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAgtac                               <  1:113944/141‑4 (MQ=255)                                                                                                    cTTGGCGGTACGGAAGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGATGGGGTAATTGAATCACGTTCTTTAGGGATGAAAGGGACt                             >  2:166290/1‑141 (MQ=255)                                                                                                                 aaGGCCTATTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTa                >  2:239156/1‑141 (MQ=255)                                                                                                                        aTTAATCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGa         <  1:139524/141‑1 (MQ=255)                                                                                                                            aTCGCGTCAAATGTTGTAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGaaaaa     <  2:336708/141‑1 (MQ=255)                                                                                                                             tCGCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAAt    >  2:275564/1‑141 (MQ=255)                                                                                                                               gCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTc  >  1:119280/1‑141 (MQ=255)                                                                                                                               gCATCAAATGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTc  >  1:111059/1‑141 (MQ=255)                                                                                                                                       |                                                                                                                                     GAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCTTATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTATTAATCGCATCAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCACTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACTTTCATTAAAGTTAAAAAAGAAAAATTC  >  CP000730/1274078‑1274344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑