Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,883,654 C→T G522D (GGT→GAT)  fmtB1 ← cell surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,883,6540CT100.0% 139.7 / NA 42G522D (GGT→GAT) fmtB1cell surface anchored protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/24);  total (18/24)

GTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAG  >  CP000730/1883523‑1883789
                                                                                                                                   |                                                                                                                                       
gtgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAg                                                                                                                                >  1:191749/1‑141 (MQ=255)
gtgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAg                                                                                                                                >  2:4633/1‑141 (MQ=255)
 tgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGt                                                                                                                               <  1:117387/141‑1 (MQ=255)
 tgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGt                                                                                                                               <  2:142916/141‑1 (MQ=255)
 tgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGt                                                                                                                               <  2:318629/141‑1 (MQ=255)
        gTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTa                                                                                                                        <  1:7656/141‑1 (MQ=255)
           ttATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGCTGTCGGCACATTATTTACGCGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAAtt                                                                                                                     <  2:374548/141‑1 (MQ=255)
              taggTTAATGTCTCGTTAAATGTTAGTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCTAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGtt                                                                                                                  <  2:343869/138‑1 (MQ=255)
                 gTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATc                                                                                                               >  2:236216/1‑141 (MQ=255)
                  ttAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATca                                                                                                              >  2:115926/1‑141 (MQ=255)
                   tAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATcac                                                                                                             >  1:61154/1‑141 (MQ=255)
                    aaTGTCTCGTTAAACGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATcaca                                                                                                            <  2:339463/141‑1 (MQ=255)
                     aTGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCacac                                                                                                           <  2:183048/141‑1 (MQ=255)
                          tcGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGAttt                                                                                                      >  2:289007/1‑141 (MQ=255)
                           cGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTtg                                                                                                     <  1:323607/141‑1 (MQ=255)
                              tAAATGTTACTTTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTCAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTtgctg                                                                                                  <  1:44240/141‑1 (MQ=255)
                                   gTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGcatca                                                                                             >  1:51122/1‑141 (MQ=255)
                                     tACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCata                                                                                           <  1:396542/141‑1 (MQ=255)
                                      aCTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCatat                                                                                          >  2:150310/1‑141 (MQ=255)
                                      aCTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCatat                                                                                          >  1:10393/1‑141 (MQ=255)
                                          ttCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGATATCACACGATTTGCTGCATCATATGTaa                                                                                      >  2:382203/1‑141 (MQ=255)
                                              tGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACAtt                                                                                  >  2:260633/1‑141 (MQ=255)
                                                 tgtCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCat                                                                               >  2:286399/1‑141 (MQ=255)
                                                    cGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATc                                                                            <  2:235250/141‑1 (MQ=255)
                                                         caTTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATGTGCTGCATCATATGTAACATTCATATCATTaa                                                                       <  2:157832/141‑1 (MQ=255)
                                                           ttattTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACa                                                                     <  2:254841/141‑1 (MQ=255)
                                                           ttattTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACa                                                                     <  1:230446/141‑1 (MQ=255)
                                                             attTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATc                                                                   <  2:126207/141‑1 (MQ=255)
                                                                        aTTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAAt                                                        <  1:369095/141‑1 (MQ=255)
                                                                                 ttAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTg                                               <  2:61154/141‑1 (MQ=255)
                                                                                            aTTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTa                                    >  1:275365/1‑141 (MQ=255)
                                                                                               ttATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTc                                 >  2:124615/1‑141 (MQ=255)
                                                                                                 aTCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCaa                               <  2:150496/141‑1 (MQ=255)
                                                                                                  tCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAAt                              >  1:403025/1‑141 (MQ=255)
                                                                                                       cATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAAtt                         <  2:191749/141‑1 (MQ=255)
                                                                                                         tAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGt                       >  2:328876/1‑141 (MQ=255)
                                                                                                            gTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTca                    <  1:149693/141‑1 (MQ=255)
                                                                                                            gTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTca                    <  1:118027/141‑1 (MQ=255)
                                                                                                                      agagTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAac          <  2:215313/141‑1 (MQ=255)
                                                                                                                      agagTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAac          <  1:146835/141‑1 (MQ=255)
                                                                                                                          tttGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCt      >  2:32789/1‑141 (MQ=255)
                                                                                                                              cTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAg  >  2:234995/1‑141 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAG  >  CP000730/1883523‑1883789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑