| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 2,274,965 | G→C | E106D (GAG→GAC) | bglG → | transcriptional antiterminator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,274,965 | 0 | G | C | 97.4% | 134.2 / NA | 39 | E106D (GAG→GAC) | bglG | transcriptional antiterminator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (19/19); minor base A (1/0); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAT > CP000730/2274837‑2275096 | cTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACaa > 2:396896/1‑141 (MQ=255) cTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCTAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTg > 1:351933/1‑141 (MQ=255) gATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGc < 1:350660/141‑1 (MQ=255) gATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGc < 1:104815/141‑1 (MQ=255) gATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGc > 2:86894/1‑141 (MQ=255) tttAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGt < 2:218924/141‑1 (MQ=255) tttAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCAGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAACGAACCAGTTAAACAATATAGTTTAACGCAAGCACTTGGCGt > 2:273468/1‑141 (MQ=255) aaaCGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAACATAGTTTAGCGCAAGAAATTGGCGTTTc > 2:393967/1‑141 (MQ=255) cGATTTGAAGCAATCGATTGCACAACATCAAACCATTGGCTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGt < 2:335751/141‑1 (MQ=255) gATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTTATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTc < 1:20562/141‑1 (MQ=255) ttgtGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGTAAGTAATTATTATATACGCTTTGAGTCAAGCCAAGGACCCAGTTAAACAATGTAGTCTATCGCAAGAAATTGGCGTTTCTGTcc > 2:100844/4‑141 (MQ=255) aTTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTcc < 1:372528/141‑1 (MQ=255) aTTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTcc < 1:298501/141‑1 (MQ=255) tGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGa > 2:369903/1‑141 (MQ=255) gAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATTTAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGAc < 2:155766/141‑1 (MQ=255) aaGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACt > 2:296483/1‑141 (MQ=255) aaGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACt > 1:170999/1‑141 (MQ=255) gCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACttt < 1:369132/141‑1 (MQ=255) caacaTCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTa < 2:247558/141‑1 (MQ=255) tCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgatga < 1:403968/141‑1 (MQ=255) tCAAACCATTGACTTATCTGTTAAAGAGCATAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAgatga < 1:15029/141‑1 (MQ=255) ccATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTag > 2:315135/1‑141 (MQ=255) cTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGa > 2:203363/1‑141 (MQ=255) ttGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATa > 1:107199/1‑141 (MQ=255) gagCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTAc < 1:375881/141‑1 (MQ=255) gCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCa > 1:10077/1‑141 (MQ=255) aGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAAc < 2:46989/141‑1 (MQ=255) gAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACt < 2:261751/141‑1 (MQ=255) gAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACt < 2:47494/141‑1 (MQ=255) aaTTATTATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATCGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCttt < 2:184359/141‑1 (MQ=255) attattATATACGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTa > 1:287736/1‑141 (MQ=255) atataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGa > 1:403615/1‑141 (MQ=255) atataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGa > 1:189969/1‑141 (MQ=255) atataCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGATTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTATCAACTATCTTTATCTCGa > 1:164375/1‑141 (MQ=255) taCGCTTTGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAg > 1:232588/1‑141 (MQ=255) ttGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGc < 1:263073/141‑1 (MQ=255) tGATACAAGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCg > 1:335546/1‑141 (MQ=255) aaaGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGc < 2:111375/140‑1 (MQ=255) aGCCAAGGACCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAt > 1:327566/1‑141 (MQ=255) | CTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATTGCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCTTTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCTGTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTATCTTTATCTCGAAAGCGTGGCGAAGGCAT > CP000730/2274837‑2275096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |