Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,342,380	A→C	L78V (TTG→GTG)	USA300HOU_2199 ←	possible HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,342,380	0	A	C	100.0%	102.3 / NA	29	L78V (TTG→GTG)	USA300HOU_2199	possible HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/15);  total (14/15)

GACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATT  >  CP000730/2342243‑2342511                                                                                                                                          |                                                                                                                                    gACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTGTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATc                                                                                                                                  >  1:278053/1‑141 (MQ=255)     ttATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGaa                                                                                                                              >  2:271600/1‑141 (MQ=255)      tataTTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGaaa                                                                                                                             <  2:84510/141‑1 (MQ=255)       atatTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGaaaa                                                                                                                            >  1:167430/1‑141 (MQ=255)             cgcAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATg                                                                                                                      >  2:48338/1‑141 (MQ=255)              gcAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGt                                                                                                                     >  2:185503/1‑141 (MQ=255)                  aaaTATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATg                                                                                                                 <  1:262454/141‑1 (MQ=255)                      atGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGttt                                                                                                             >  2:88367/1‑141 (MQ=255)                         aCGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGGAAAGATATGTAATGGTTTaaa                                                                                                          >  1:331874/1‑141 (MQ=255)                         aCGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATAGGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTaaa                                                                                                          <  2:136538/141‑1 (MQ=255)                           gTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTATAACTCACATCAGAAAAGATATGTAAATGTTTAAACg                                                                                                        >  2:187052/1‑141 (MQ=255)                              atatatCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCa                                                                                                     <  2:86326/141‑1 (MQ=255)                                      tcaatatcttcaaATAAAAATGGTTCGCTCAATACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACt                                                                                             <  2:380921/141‑1 (MQ=255)                                         atatcttcaaCTAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTACTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGa                                                                                          >  2:99938/1‑141 (MQ=255)                                              ttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCACAAAAGCTATGACATGGTTTAAACGTCATATTAACTTGATCCAt                                                                                     <  2:121556/141‑1 (MQ=255)                                                     aaaaaTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATcc                                                                              >  2:192531/1‑141 (MQ=255)                                                       aaaTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCAt                                                                            >  1:154436/1‑141 (MQ=255)                                                         aTGGTTCTCTCAAGACTTTCTTTTTATATGTTTATGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGACCTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGa                                                                          <  2:313103/141‑1 (MQ=255)                                                                           tctttTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTc                                                        <  2:344928/141‑1 (MQ=255)                                                                             tttttATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCt                                                      >  1:14014/1‑141 (MQ=255)                                                                               tttATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGt                                                    <  2:62601/141‑1 (MQ=255)                                                                                           tttacgatcATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGt                                        <  2:210319/133‑1 (MQ=255)                                                                                            taTTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTg                                       <  1:156502/140‑1 (MQ=255)                                                                                               tCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATc                                    <  1:66195/141‑1 (MQ=255)                                                                                                     tatTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCa                              <  1:218086/141‑1 (MQ=255)                                                                                                                   ccAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTAttagttag                >  2:203486/1‑141 (MQ=255)                                                                                                                            cATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCcaa       <  2:222020/141‑1 (MQ=255)                                                                                                                             aTTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCcaac      >  2:402676/1‑141 (MQ=255)                                                                                                                                 tAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAAtt  <  2:251615/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATT  >  CP000730/2342243‑2342511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑