Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,388,866	A→G	G8G (GGA→GGG)	USA300HOU_2249 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,388,866	0	A	G	100.0%	110.6 / NA	32	G8G (GGA→GGG)	USA300HOU_2249	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

TCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAAT  >  CP000730/2388729‑2389000                                                                                                                                          |                                                                                                                                       tcctATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCaa                                                                                                                                     <  1:250557/141‑1 (MQ=255)      tGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACttgt                                                                                                                                <  2:50033/141‑1 (MQ=255)          tcttctTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTgttgtt                                                                                                                            >  1:55533/1‑141 (MQ=255)           cttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGttt                                                                                                                           <  1:296862/141‑1 (MQ=255)                tAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCg                                                                                                                      >  2:20082/1‑141 (MQ=255)                   aTGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTTGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGaca                                                                                                                   >  1:262920/1‑141 (MQ=255)                    tGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGacaa                                                                                                                  <  2:276764/141‑1 (MQ=255)                     gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGacaac                                                                                                                 >  2:173487/1‑141 (MQ=255)                            aTTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTtata                                                                                                          <  1:360779/141‑1 (MQ=255)                             ttCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATAc                                                                                                         <  1:249062/141‑1 (MQ=255)                               cATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCt                                                                                                       >  2:113909/1‑141 (MQ=255)                                  attattatACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTaaa                                                                                                    <  1:312804/141‑1 (MQ=255)                                                      aTTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCaa                                                                                >  1:414269/1‑141 (MQ=255)                                                          attatATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGc                                                                            <  2:174503/141‑1 (MQ=255)                                                           ttatATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCa                                                                           >  2:327561/1‑141 (MQ=255)                                                             atatatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATg                                                                         >  1:275446/1‑141 (MQ=255)                                                               atatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGa                                                                       <  1:99374/141‑1 (MQ=255)                                                               atatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGa                                                                       <  1:412484/141‑1 (MQ=255)                                                                tatCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAg                                                                      >  2:361338/1‑141 (MQ=255)                                                                            cATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAAt                                                          >  2:352472/1‑141 (MQ=255)                                                                              ttttATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGt                                                        >  2:173179/1‑141 (MQ=255)                                                                                           aaaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTa                                           <  1:195154/141‑1 (MQ=255)                                                                                            aaTATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTaa                                          <  1:20082/141‑1 (MQ=255)                                                                                                ttAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGAt                                      >  1:45238/1‑141 (MQ=255)                                                                                                         tGGCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAAGATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGt                             >  2:405823/1‑141 (MQ=255)                                                                                                           gCTATATCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAg                           <  2:272222/141‑1 (MQ=255)                                                                                                                 tCTTGTTTAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTc                     <  1:173487/141‑1 (MQ=255)                                                                                                                      tttAGATTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAag                >  1:173929/1‑141 (MQ=255)                                                                                                                           aTTTATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGCACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTgg           <  2:246838/141‑1 (MQ=255)                                                                                                                            tttATATATTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACACCTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTCAAGATTTATTATGTAGTGATTCTTAAGAGTggg          <  1:163226/141‑1 (MQ=255)                                                                                                                                 atatTTTGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGa     >  2:343559/1‑141 (MQ=255)                                                                                                                                    ttttGGGCAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAAt  >  2:32622/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                       TCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATATTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTAAAAAAGATATATTACAACTCAATGGCATGGAGTGTGACATCAATGTTAAAGAACCACTAAAGATTTATTATGTAGTGATTCTTAAGAGTGGGATAGAAAT  >  CP000730/2388729‑2389000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑