BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,444	C→T	53.2%	R36R (AGG→AGA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,444	0	C	T	53.2%	4.3 / 185.1	160	R36R (AGG→AGA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (42/33); new base T (51/33); total (94/66)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

Only 100 of 164 total aligned reads displayed.

TATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATAC  >  CP000730/36306‑36581

tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                                                          <  1:197902/140‑1 (MQ=18)

tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                                                          >  1:65373/1‑140 (MQ=18)

tATTGTGATGTACGTCTCATCAATACACCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                                                          >  1:218039/1‑140 (MQ=255)

  ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTTCCtt                                                                                                                                        >  2:149085/1‑140 (MQ=19)

  ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                        <  1:70228/140‑1 (MQ=21)

  ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                        <  1:133390/140‑1 (MQ=18)

  ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                        >  2:199742/1‑140 (MQ=40)

  ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                        <  2:287568/140‑1 (MQ=11)

   tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                                                       <  2:92941/140‑1 (MQ=11)

    ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGGATATTCTTGAACCCAACGGTAGATCGTTGACTGATGAACGTGTACCCCACGTTCTCTTaa                                                                                                                                      >  2:246255/1‑140 (MQ=11)

    ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                      <  1:15026/140‑1 (MQ=11)

    ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                      <  2:230953/140‑1 (MQ=11)

    gtGATATCCGTCTCCGCAATACGCCAGTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                      <  1:50778/139‑1 (MQ=11)

      gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                                                                                    >  1:116610/5‑140 (MQ=255)

      gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAATTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACC‑CGTTTCCtctaatt                                                                                                                                   >  2:10929/1‑134 (MQ=11)

      gATGTACGTCTCATCAATACGCCATTTGTAATAAGCATTTTTATGCTTTTTTTTCCATATGTGATACAAAATTAGGGGATATTATTAAAAACTAAGGCAGACCTACGAATGATGAACGTTTACACCACGGCCCCTTAata                                                                                                                                    >  2:52059/1‑140 (MQ=2)

      gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGCTGAATGATGAACGTTTACACCACATGCTCTAAata                                                                                                                                    >  1:266914/1‑140 (MQ=11)

        tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGGTCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                                                                                  >  1:128574/3‑140 (MQ=11)

        tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGATCTCTTAATAtt                                                                                                                                  >  1:88773/3‑140 (MQ=11)

         atACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAAGGTAGATAGTTGAATGTTGAACGTTTACACCACGTTCTCTTAATAttt                                                                                                                                 <  1:303618/139‑1 (MQ=11)

          tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                                                                                >  1:148137/1‑140 (MQ=21)

          tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                                                                                >  1:156506/1‑140 (MQ=21)

          tACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTATTAATATTTc                                                                                                                                >  2:294951/1‑140 (MQ=11)

           aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                                               >  1:115521/1‑140 (MQ=255)

            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                                              >  1:239764/1‑140 (MQ=255)

            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAGTATTTCAg                                                                                                                              >  2:137557/1‑140 (MQ=25)

            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGCGGCATATTCTTGCACCCGATTCAGGGTCGTTGAATGATGGACGTTTACACCACGTTCTCTTAATCTTTCAg                                                                                                                              >  1:60727/1‑140 (MQ=11)

            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                                                                              >  1:294509/1‑140 (MQ=255)

              tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                            <  2:259774/140‑1 (MQ=255)

               ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGata                                                                                                                           <  1:62178/140‑1 (MQ=255)

                tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTAAGatat                                                                                                                          >  2:101887/1‑140 (MQ=25)

                 catccATACCCCATTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAATTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                                                                                         <  1:15850/140‑1 (MQ=11)

                 catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGGTGCATATTCTTGATCCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                                                                                         <  1:79041/140‑1 (MQ=11)

                    caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                                                      >  1:275551/1‑140 (MQ=255)

                    caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                                                      >  1:212669/1‑140 (MQ=255)

                    caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTCAACCCAACGGTCGATCGTTCAATGATGAACGTTTACACCACGTTCTCTTACTATTTCAGatatatac                                                                                                                      >  1:144118/1‑138 (MQ=11)

                    caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTGGACCGTTGAATGATGAACGTTTACTCCACGTTCCCTTAATATTTCAGttatatga                                                                                                                      >  1:4373/1‑138 (MQ=255)

                     aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTTGAACCCAACGGTAGTTCGTTGAATTATGCACGTTTACACACCGTTCTCTTAATCTTTAAGGTATATCAc                                                                                                                     >  1:26026/1‑140 (MQ=11)

                      aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGCTCGTTGAATGATGAACGTTTAAACTACGTTCTCTTAATATTTGAGATATATCagg                                                                                                                    >  1:123760/1‑138 (MQ=11)

                       tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                   >  1:209704/1‑140 (MQ=255)

                       tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                   <  2:35503/140‑1 (MQ=255)

                       tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATCTTTCAGATATATCCCGa                                                                                                                   >  2:132764/1‑140 (MQ=255)

                       tACGCCATTTGTAACAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCATATATATCACGa                                                                                                                   <  2:156387/140‑1 (MQ=11)

                        aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATGAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                                                  <  2:218631/140‑1 (MQ=21)

                        aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACTTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                                                  <  2:116214/140‑1 (MQ=11)

                        aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                                                  >  2:88394/1‑140 (MQ=255)

                         cGCCGTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                                                                                                 <  2:232443/140‑1 (MQ=16)

                         cGCCATTTGTAATAGGCTGGTTTATGCTTGTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                                                 >  2:316944/1‑140 (MQ=11)

                         cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATCGACTTACGCCATATTCTTGAACCCAACGGGAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                                                 <  1:152063/140‑1 (MQ=11)

                         cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                                                 >  1:54145/1‑140 (MQ=255)

                         cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                                                                                                 <  2:300701/140‑1 (MQ=21)

                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                <  1:251491/140‑2 (MQ=255)

                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                <  1:251503/140‑2 (MQ=255)

                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                <  1:37780/140‑2 (MQ=255)

                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                >  1:307523/1‑139 (MQ=255)

                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCCGATATTTCGCGATaa                                                                                                                >  1:296488/1‑140 (MQ=11)

                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                >  2:112231/1‑140 (MQ=25)

                           ccttttGTAATAAGCTTTTTTATGCGTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                               <  2:164249/137‑1 (MQ=255)

                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAccagagc                                                                                                               >  1:221069/1‑134 (MQ=11)

                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                                               <  1:33903/140‑3 (MQ=11)

                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                                               >  1:201465/1‑138 (MQ=11)

                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                                               >  1:283019/1‑138 (MQ=255)

                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                               >  2:253726/1‑140 (MQ=25)

                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGATAAc                                                                                                               >  2:90814/1‑140 (MQ=255)

                           ccACTTGTAATAAGCTTTTTTATGCATTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGATCGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                                               <  1:97096/140‑3 (MQ=11)

                             aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTCTATACAGTCCTTTTTCACCTCGTTCCATTAATATGTCAAATATATCACGATAACTc                                                                                                             >  1:231109/1‑140 (MQ=11)

                              tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                            <  1:69881/140‑1 (MQ=21)

                              tttGTAATAAGCTTTTTTAGGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                                                            >  1:192272/1‑140 (MQ=11)

                               ttGGAATCAGCTTTTTTATGCTTTTTCTTCCAACTTTGATGCAAAATGTGGCCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                           <  2:66765/140‑1 (MQ=11)

                                tGTACTAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCCCAAAAGCTCAAt                                                                                                          >  2:276793/1‑140 (MQ=11)

                                 gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATCAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                         <  2:306129/140‑1 (MQ=21)

                                 gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCGACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                         >  2:35310/1‑140 (MQ=11)

                                 gTAATAAGCTTTTTTATGACTATTATACCAAATACGAGGTAAAATTTGGGCATATTGTTGACCCCAACAGTAGGGCTGTTAAGGGGCGGCCTTGACACCACTTTCCCGTTATATTTCAGATATATCACGATAACTCAATg                                                                                                         <  2:27888/140‑1 (MQ=2)

                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAAACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTAAGCTATATCATGATAACTCaacac                                                                                                        >  2:50910/1‑137 (MQ=11)

                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGTTATATCACCATAGCTCAATgc                                                                                                        >  2:41429/1‑139 (MQ=11)

                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATGTCACGATAACTCAATgc                                                                                                        >  1:262844/1‑139 (MQ=16)

                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCACTgc                                                                                                        >  1:229535/1‑139 (MQ=16)

                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGACCGTTTACACCACGTTCTCTTAATATTTCCGATATATCACGATAGCTCAATgca                                                                                                       >  1:46820/1‑138 (MQ=11)

                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                       >  2:125489/1‑138 (MQ=11)

                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                       <  2:279768/140‑3 (MQ=11)

                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACCATAGCTCAATgca                                                                                                       >  1:13704/1‑138 (MQ=11)

                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTGTACTCCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                       <  2:176336/140‑3 (MQ=11)

                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATGTGATATAAAATTGGAGCATAGTCTTCACAGCAACGATTGACCGTTGAATGATGAACGTTTACACCACGATCCCTTAATATTTCAAATATATCACAATAACTCAAtaca                                                                                                       >  2:286840/1‑137 (MQ=11)

                                                aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAACGTATATCTTAGAtagt                                                                                          <  1:158796/140‑1 (MQ=25)

                                                 tGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                         <  2:295903/140‑1 (MQ=35)

                                                  gCTTGTTCTTCCCATTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagtag                                                                                        <  2:133656/140‑1 (MQ=255)

                                                           tcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATAAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTAAATGAATATCTTAGATAGTAGCCAACGGCt                                                                               >  2:66217/1‑140 (MQ=12)

                                                                  ttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAgaga                                                                        >  1:3088/1‑140 (MQ=25)

                                                                  ttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGa                                                                        <  2:186893/140‑1 (MQ=35)

                                                                              tGGGGGATATTCTGGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                            <  2:23722/140‑1 (MQ=17)

                                                                                 ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                         >  2:61128/1‑140 (MQ=32)

                                                                                 ggCATATTCTTAAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                         <  1:91274/140‑1 (MQ=18)

                                                                                             aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATc                                             >  1:245500/1‑140 (MQ=33)

                                                                                                ccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa                                          <  2:275889/140‑1 (MQ=33)

                                                                                                   aCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAAt                                       <  2:295702/140‑1 (MQ=33)

                                                                                                   aCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAATTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAAt                                       >  2:205027/1‑140 (MQ=18)

                                                                                                                       gaACGTTTACACCACGTTCTGTTAATATTTCAGATATATCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAGTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCt                   >  1:207628/1‑140 (MQ=255)

                                                                                                                       gaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCt                   >  2:275793/1‑140 (MQ=32)

                                                                                                                              tACAACACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt            <  2:86624/140‑1 (MQ=17)

                                                                                                                                caccacGTTCCCTTAATCTTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATACTTGTTAAATTGTTTATATCTGAAATAGCTGCTACAGAAGACTCCTTTTTGTTaa          >  1:296678/1‑140 (MQ=11)

TATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATAC  >  CP000730/36306‑36581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`