Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,265 C→T 31.5% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2650CT31.5% 254.8 / 162.4 237intergenic (+323/‑811)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (89/71);  new base T (31/43);  total (121/116)
Fisher's exact test for biased strand distribution p-value = 6.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

Only 100 of 239 total aligned reads displayed.

AGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAG  >  CP000730/76126‑76403
                                                                                                                                           |                                                                                                                                          
                           ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                                                                                                 <  1:70295/135‑1 (MQ=255)
                           aaatgaGGTTCTGTTGAAAAGTTGAATTTATAGTATAACTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                                                                                                                 <  1:26498/135‑1 (MQ=11)
                           aaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                                                                                                 <  2:36335/140‑1 (MQ=40)
                            aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                                                                                                                <  1:9737/136‑1 (MQ=9)
                             tataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTGACAAAACGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                                                                                               >  2:207613/1‑140 (MQ=255)
                             atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                                                                                               <  1:202561/137‑1 (MQ=9)
                                              aGTTGAATCTATAATATAAATATAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTg                                                                                              <  2:222084/140‑1 (MQ=255)
                                               gTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa                                                                                             <  1:120218/140‑1 (MQ=35)
                                                    atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAatat                                                                                        <  2:255704/137‑1 (MQ=2)
                                                    atttataGTATAATTTTAACAAAAAGGAGTATTCTGAATGAGCTATTTCCGCTATAAAAAATTAAACAAGGATAAAATCAATGTAATTGTTGACAACTATCCTAGATAAGCTTGAAGTGATCGTGATATACCTGAAatat                                                                                        >  2:75540/4‑140 (MQ=0)
                                                     tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAAATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                       >  2:83956/3‑140 (MQ=2)
                                                      ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTa                                                                                      >  1:88394/2‑140 (MQ=9)
                                                       tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGTATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                     <  1:300607/140‑1 (MQ=255)
                                                        ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg                                                                                    <  1:218961/140‑1 (MQ=12)
                                                         taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg                                                                                   >  1:266688/1‑140 (MQ=33)
                                                          aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTTGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg                                                                                  <  1:233645/140‑1 (MQ=18)
                                                           gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                 >  1:102698/1‑140 (MQ=17)
                                                           gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                 <  1:57867/140‑1 (MQ=33)
                                                           atataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATAACTGAAATATTAAgaga                                                                                 <  1:158597/139‑1 (MQ=255)
                                                             ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAAAATTAAGAGAAc                                                                               >  1:38671/1‑140 (MQ=16)
                                                              taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAATGGAACg                                                                              >  1:126212/1‑140 (MQ=16)
                                                              taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACg                                                                              >  2:63854/1‑140 (MQ=18)
                                                              taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                              >  1:35310/1‑140 (MQ=12)
                                                               aaTTTTACCAAAACGGTGTCTTCGGTATGAACTATTTCAGATATAATCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                             <  2:246284/140‑1 (MQ=11)
                                                                aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                            >  1:235807/1‑140 (MQ=16)
                                                                     aaCAAAAGGGAGGCTTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTa                                                                       >  2:187857/1‑140 (MQ=11)
                                                                     aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTa                                                                       >  1:31394/1‑140 (MQ=17)
                                                                      aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATAAAGGGAACGTGGTGTaa                                                                      >  2:302352/1‑140 (MQ=12)
                                                                      aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                      >  1:309771/1‑140 (MQ=33)
                                                                      aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                      >  2:248644/1‑140 (MQ=33)
                                                                      aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                      >  1:275268/1‑140 (MQ=16)
                                                                      aCAAAAAGGAATCTTCTGTATGAACTATTTCGGATATAAACAATTTAACAAGGATGTTATCACTGTAGCAGTTGCCTACTATCTAAGATATGCATTGAGCTATCGTGATCTCTCTGAAAGCTTAAGAGAACGTGgtgtca                                                                      >  2:216176/1‑138 (MQ=11)
                                                                       cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGGGTaaa                                                                     >  2:113510/1‑140 (MQ=17)
                                                                       cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                     >  2:116729/1‑140 (MQ=16)
                                                                       cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                     >  2:212669/1‑140 (MQ=12)
                                                                        aaaaaGGAGTCTTCTATATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAAc                                                                    >  1:238234/1‑140 (MQ=12)
                                                                          aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGt                                                                  >  1:302607/1‑140 (MQ=18)
                                                                           aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtt                                                                 >  2:280799/1‑140 (MQ=12)
                                                                           aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAAAATTAAGCGAACTTGGTGTAAACGtt                                                                 >  2:88773/1‑140 (MQ=255)
                                                                            aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtcc                                                                >  2:117590/1‑138 (MQ=16)
                                                                              gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATTTTATCTCTGTAGCCGTTGGCTACTATCTACGATATGCATTGCGCTATCGTGATATATCTGAAATATTAAGAGAACGTTGTGTAAACGTtcat                                                              <  2:127036/140‑1 (MQ=11)
                                                                                  tttctGTATGAACTATTTCAAATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCAtt                                                          <  1:212546/139‑1 (MQ=255)
                                                                                  cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                          <  2:129673/140‑1 (MQ=33)
                                                                                  cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTACCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAGACGTTCATCAtt                                                          >  1:294951/1‑140 (MQ=255)
                                                                                     ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAACAGTTCCTCATTCaa                                                       >  1:110729/1‑140 (MQ=12)
                                                                                     ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCaa                                                       >  1:143023/1‑140 (MQ=16)
                                                                                        tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                    <  2:15457/140‑1 (MQ=32)
                                                                                        gaTGAACTATTTTAGATATAAACAATTTATCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                    <  1:136803/139‑1 (MQ=12)
                                                                                          tGAACTATTTCAGATATCAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATc                                                  <  2:58418/140‑1 (MQ=11)
                                                                                             aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                               <  2:294087/140‑1 (MQ=18)
                                                                                             aCTATTGCAGATTTAAACAATTTATCAAGGATGTTATCACTGTCGCTGTTGGCTACTATCTAATATATGCATTGAGTTATCGTGATATATCTGAAAGATTAAGGGAACGTTGTGTAAACGTTCATCATTCAACGGTCTAc                                               <  2:163763/140‑1 (MQ=11)
                                                                                              cTATTTCAGATATTAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                              <  2:17248/140‑1 (MQ=255)
                                                                                              cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                              >  2:96061/1‑140 (MQ=12)
                                                                                                 tttCAGTTATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                           <  2:168418/140‑1 (MQ=18)
                                                                                                 tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCTTTCAACGGTCTACCGtt                                           >  1:129023/1‑140 (MQ=12)
                                                                                                 tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                           >  1:191640/1‑140 (MQ=18)
                                                                                                 tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                           >  1:184937/1‑140 (MQ=18)
                                                                                                 tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCACCGATCTACCGtt                                           >  1:276793/1‑140 (MQ=255)
                                                                                                  ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                          <  2:271093/140‑1 (MQ=255)
                                                                                                    tagattaaaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                        <  2:111326/133‑1 (MQ=11)
                                                                                                     aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                       <  2:52155/140‑1 (MQ=255)
                                                                                                      cctataAACAATTTAACAAGGTTGTTATCAATGTAGTTGTTGGCTACTATCTAGGTTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                      <  1:4369/138‑1 (MQ=11)
                                                                                                        tataAACAATTTAACAGGGATGTTATCACTGTAGCTGTGGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                    >  1:178844/1‑140 (MQ=12)
                                                                                                        tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTTAGCTATCGTGATATATCTGAAATATTAAAAGAACGAGGTGTAAGAGTTCATGATTCAACGACCTACAATTGGGTTCa                                    >  2:311065/1‑140 (MQ=11)
                                                                                                        tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                    <  1:173235/140‑1 (MQ=255)
                                                                                                         ataAACTATTTAACAAGGATGTTATCACTGTAGCTGGTGGCTACTATCTAAGATATGCATTGTGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCaa                                   <  1:216022/140‑1 (MQ=255)
                                                                                                          tgaaCAATTTAACAGGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                  <  2:70763/138‑1 (MQ=11)
                                                                                                          taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                  <  2:278180/140‑1 (MQ=18)
                                                                                                          taAACAATTGAACAAGGAAGTAATAAATGTAGACGATGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                  >  2:224199/1‑140 (MQ=11)
                                                                                                           aaaCAATTTAACAATGATTTTATCATTGTAGCCTTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                 <  2:258325/140‑1 (MQ=11)
                                                                                                           aaaCAATTTAACAAGGATGTTATCACTGCAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                 <  2:220332/140‑1 (MQ=255)
                                                                                                                aTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                            >  2:288488/1‑140 (MQ=18)
                                                                                                                aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                            <  2:266688/140‑1 (MQ=33)
                                                                                                                aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                            >  1:118598/1‑140 (MQ=12)
                                                                                                                aTTTAACAAGGATGTTATACCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                            <  2:1121/140‑1 (MQ=11)
                                                                                                                 tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                           <  1:242521/140‑1 (MQ=255)
                                                                                                                  ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                          <  1:115204/140‑1 (MQ=255)
                                                                                                                  ttAAAAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                          <  2:33206/140‑1 (MQ=18)
                                                                                                                    aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                        >  1:147898/1‑140 (MQ=255)
                                                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                       >  1:258688/1‑140 (MQ=16)
                                                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                       >  2:150616/1‑140 (MQ=255)
                                                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCACCa                       >  1:22903/1‑140 (MQ=255)
                                                                                                                         ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAttt                   <  1:248644/140‑1 (MQ=32)
                                                                                                                         ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                   <  2:31394/140‑1 (MQ=12)
                                                                                                                          gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                  >  1:59969/1‑140 (MQ=255)
                                                                                                                            tGTCATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGGGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                <  2:47737/140‑1 (MQ=11)
                                                                                                                              ttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat              <  1:23235/140‑1 (MQ=25)
                                                                                                                              ttATCACCGTAGCTGTTGGCTAATACTTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAACCGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat              <  1:75910/140‑1 (MQ=11)
                                                                                                                                attaCTGTAGCTGTTGGCTATTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa            <  1:107409/137‑1 (MQ=255)
                                                                                                                                agcACTGTAGCCGTTGGCTAATATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa            <  2:197292/138‑1 (MQ=11)
                                                                                                                                aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGATGGGTTCAAGAATATGCCCCAATTTTATATCa            >  1:91139/1‑140 (MQ=19)
                                                                                                                                aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa            <  1:199076/140‑1 (MQ=255)
                                                                                                                                 tCACTGTAGCCGTTGGCTACTATCTAAGATATGCCTTGCGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTACATCATTCAACGCTATACCGTTGGGTTCAAGAAGATGCGCCAATTTTATATCaa           >  2:142336/1‑140 (MQ=11)
                                                                                                                                  cACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa          >  2:44168/1‑140 (MQ=37)
                                                                                                                                  cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa          >  1:290341/1‑140 (MQ=255)
                                                                                                                                      gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg      >  1:269639/1‑140 (MQ=25)
                                                                                                                                      gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg      >  2:231337/1‑140 (MQ=255)
                                                                                                                                        aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga    <  2:232992/140‑1 (MQ=255)
                                                                                                                                         gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAGCGTAGGGTTTAAGAATATGCCCCAATTTTATATCAAGTTTGga  >  1:59392/1‑139 (MQ=11)
                                                                                                                                          ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag  >  1:272993/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAG  >  CP000730/76126‑76403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: